Efficiency evaluation of immunization protocol with Echinococcus granulosus antigen B (AgB)

2017 ◽  
Author(s):  
Nisreen Mustafa ◽  
Wafaa Shani
Keyword(s):  
Echinococcus Granulosus ◽  
Efficiency Evaluation ◽  
Immunization Protocol ◽  
Antigen B

scholarly journals Echinococcus granulosus Antigen B binds to monocytes and macrophages modulating cell response to inflammation

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Valeria Silva-Álvarez ◽  
Ana Maite Folle ◽  
Ana Lía Ramos ◽  
Eduardo S. Kitano ◽  
Leo K. Iwai ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Cell Response ◽  
Antigen B ◽  
Monocytes And Macrophages

Cloning and Expression of Two Genes Encoding Subunits of Echinococcus granulosus Antigen B.

2006 ◽  
Vol 9 (3) ◽  
pp. 350-354 ◽  
Author(s):  
Ramin Pazoki ◽  
Bahram Kazemi ◽  
Mohammad Reza Nazar ◽  
Jafar Masoud ◽  
Mohammad Reza Akbar
Keyword(s):  
Echinococcus Granulosus ◽  
Cloning And Expression ◽  
Antigen B ◽  
Genes Encoding

scholarly journals Echinococcus granulosus Antigen B Impairs Human Dendritic Cell Differentiation and Polarizes Immature Dendritic Cell Maturation towards a Th2 Cell Response

2007 ◽  
Vol 75 (4) ◽  
pp. 1667-1678 ◽  
Author(s):  
Rachele Riganò ◽  
Brigitta Buttari ◽  
Elisabetta Profumo ◽  
Elena Ortona ◽  
Federica Delunardo ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Echinococcus Granulosus ◽  
Therapeutic Option ◽  
Costimulatory Molecule ◽  
Lower Percentage ◽  
Th2 Response ◽  
Monocyte Differentiation ◽  
Dendritic Cell Differentiation ◽  
Antigen B ◽  
Dc Maturation

ABSTRACT Despite inducing a strong host cellular and humoral immune response, the helminth Echinococcus granulosus is a highly successful parasite that develops, progresses, and ultimately causes chronic disease. Although surgery remains the preferred therapeutic option, pharmacological research now envisages antihelminthic strategies. To understand the mechanisms that E. granulosus uses to escape host immunosurveillance and promote chronic infection, we investigated how two hydatid cyst components, purified antigen B (AgB) and sheep hydatid fluid (SHF), act on host dendritic cell (DC) differentiation from monocyte precursors and how they influence maturation of DC that have already differentiated. We evaluated the immunomodulatory potential of these antigens by performing immunochemical and cytofluorimetric analyses of monocyte-derived DCs from healthy human donors. During monocyte differentiation, AgB and SHF downmodulated CD1a expression and upregulated CD86 expression. Compared with immature DCs differentiated in medium alone (iDCs), AgB- and SHF-differentiated cells stimulated with lipopolysaccharide included a significantly lower percentage of CD83+ cells (P < 10−4) and had weaker costimulatory molecule expression. When stimulated with AgB and SHF, iDCs matured and primed lymphocytes towards the Th2 response typical of E. granulosus infection. SHF and particularly AgB reduced the production of interleukin-12p70 (IL-12p70) and tumor necrosis factor alpha in lipopolysaccharide-stimulated iDCs. Anti-IL-10 antibodies increased the levels of IL-12p70 secretion in AgB- and SHF-matured DCs. AgB and SHF induced interleukin-1 receptor-associated kinase phosphorylation and activated nuclear factor-κB, suggesting that Toll-like receptors could participate in E. granulosus-stimulated DC maturation. These results suggest that E. granulosus escapes host immunosurveillance in two ways: by interfering with monocyte differentiation and by modulating DC maturation.

Ultrastructural immunocytochemical localization of two hydatid fluid antigens (antigen 5 and antigen B) in the brood capsules and protoscoleces of ovine and equine Echinococcus granulosus and E. multilocularis

Parasitology ◽  
1978 ◽  
Vol 77 (2) ◽  
pp. 143-152 ◽  
Author(s):  
Caroline Davies ◽  
M. D. Rickard ◽  
D. T. Bout ◽  
J. P. Smyth
Keyword(s):  
Echinococcus Granulosus ◽  
Periodic Acid ◽  
Ultrastructural Localization ◽  
Capsule Wall ◽  
Periodic Acid Schiff ◽  
Hydatid Fluid ◽  
Antigen B ◽  
Parenchyma Cells ◽  
Perinuclear Cytoplasm ◽  
Antigen 5

SummaryThe unlabelled antibody method was used in the ultrastructural localization of two hydatid fluid antigens, antigen 5 and antigen B, in brood capsules and protoscoleces of Echinococcus granulosus and E. multilocularis. Antigen 5 was found in the parenchyma cells of the protoscolex and brood capsule wall and to a lesser extent in the walls of the flame cells and collecting ducts of the excretory system and in the surrounding interstitial material. It is suggested that, while some excretion of this antigen may occur from the protoscolex, it could also be liberated into the cystic cavity by degeneration of protoscoleces and parenchymal cells of the brood capsule wall. Antigen B was found mainly in the distal cytoplasm and perinuclear cytoplasm of the tegument anterior to the suckers. It is apparently secreted to the outside and was present in the brood capsule contents; it adheres to the anterior surface and the posterior periodic acid–Schiff (PAS)-positive glycocalyx of the protoscolex and to the inner surface of the brood capsule wall. The protoscolex tegument posterior to the suckers was negative. The parenchyma cells of the protoscolex and brood capsule wall were also positive although the intensity of the reaction product was variable.

scholarly journals Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus

2012 ◽  
Vol 5 (5) ◽  
pp. 355-357 ◽  
Author(s):  
Jahangir Abdi ◽  
Bahram Kazemi ◽  
Mohammad Hasan Karimfar ◽  
Mohammad Bagher Rokni
Keyword(s):  
Antibody Response ◽  
Echinococcus Granulosus ◽  
Rabbit Antibody ◽  
Antigen B

scholarly journals Lipid-Free Antigen B Subunits from Echinococcus granulosus: Oligomerization, Ligand Binding, and Membrane Interaction Properties

2015 ◽  
Vol 9 (3) ◽  
pp. e0003552 ◽  
Author(s):  
Valeria Silva-Álvarez ◽  
Gisela R. Franchini ◽  
Jorge L. Pórfido ◽  
Malcolm W. Kennedy ◽  
Ana M. Ferreira ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Echinococcus Granulosus ◽  
Membrane Interaction ◽  
Antigen B ◽  
B Subunits ◽  
Free Antigen
Sign in / Sign up

Export Citation Format

Share Document