scholarly journals Toxicity of 13C-labeled linoleic and linolenic acids for diagnostic breath tests

2019 ◽  
pp. 19-24
Author(s):  
Ya. Ya. Tynio ◽  
G. V. Morozova ◽  
Yu. K. Biryukova ◽  
E. V. Trubnikova ◽  
M. V. Zylkova ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Intragastric Administration ◽  
Control Groups ◽  
Subchronic Toxicity ◽  
Breath Tests ◽  
Diagnostic Use ◽  
Tract Function ◽  
Higher Doses

Noninvasive stable isotope breath tests allow highly accurate and safe estimation of liver and biliary tract function. The aim of this study was to test 13С-labeled linoleic and linolenic acids intended for diagnostic use for acute and subchronic toxicity. The acids were synthesized using the patented method. A single intragastric administration of the tested compounds to experimental BALB/c mice and Wistar rats in the amounts exceeding clinical doses 500 to 2500-fold did not cause animal death. In the subchronic toxicity test, the rats received 5 to 25 times higher doses than recommended for clinical use in humans. In a 14-day follow-up period, no significant differences were observed between the main and the control groups in terms of weight, blood count (red blood cells, white blood cells, platelets), and blood biochemistry (hemoglobin, total protein, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, bilirubin). The studied compounds are safe at doses intended for oral administration and are recommended for further preclinical and clinical trials.

scholarly journals Utility of procalcitonin, C-reactive protein and white blood cells alone and in combination for the prediction of clinical outcomes in community-acquired pneumonia

2015 ◽  
Vol 53 (4) ◽  
Author(s):  
Andriy Zhydkov ◽  
Mirjam Christ-Crain ◽  
Robert Thomann ◽  
Claus Hoess ◽  
Christoph Henzen ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Clinical Outcomes ◽  
Blood Cells ◽  
White Blood Cells ◽  
Community Acquired Pneumonia ◽  
Adverse Clinical Outcome ◽  
C Reactive Protein ◽  
Prognostic Information ◽  
Reactive Protein

AbstractThe added value of biomarkers, such as procalcitonin (PCT), C-reactive protein (CRP), and white blood cells (WBC), as adjuncts to clinical risk scores for predicting the outcome of patients with community-acquired pneumonia (CAP) is in question. We investigated the prognostic accuracy of initial and follow-up levels of inflammatory biomarkers in predicting death and adverse clinical outcomes in a large and well-defined cohort of CAP patients.We measured PCT, CRP and WBC on days 1, 3, 5, and 7 and followed the patients over 30 days. We applied multivariate regression models and area under the curve (AUC) to investigate associations between these biomarkers, the clinical risk score CURB-65, and clinical outcomes [i.e., death and intensive care unit (ICU) admission].Of 925 patients with CAP, 50 patients died and 118 patients had an adverse clinical outcome. None of the initial biomarker levels significantly improved the CURB-65 score for mortality prediction. Follow-up biomarker levels showed significant independent association with mortality at days 3, 5, and 7 and with improvements in AUC. Initial PCT and CRP levels were independent prognostic predictors of adverse clinical outcome, and levels of all biomarkers during the course of disease provided additional prognostic information.This study provides robust insights into the added prognostic value of inflammatory markers in CAP. Procalcitonin, CRP, and to a lesser degree WBC provided some prognostic information on CAP outcomes, particularly when considering their kinetics at days 5 and 7 and when looking at adverse clinical outcomes instead of mortality alone.

scholarly journals Lithium Carbonate in the Treatment of Graves’ Disease with ATD-Induced Hepatic Injury or Leukopenia

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Rendong Zheng ◽  
Kemian Liu ◽  
Kun Chen ◽  
Wen Cao ◽  
Lin Cao ◽  
...  
Keyword(s):  
Liver Function ◽  
Blood Cells ◽  
Hepatic Injury ◽  
Clinical Effect ◽  
Radioiodine Therapy ◽  
White Blood Cells ◽  
Lithium Carbonate ◽  
Graves Disease ◽  
Blood Cell Count

Objective. GD with ATD-induced hepatic injury or leukopenia occurs frequently in clinical practice. The purpose of the present study was to observe the clinical effect of lithium carbonate on hyperthyroidism in patients with GD with hepatic injury or leukopenia.Methods. Fifty-one patients with GD with hepatic injury or leukopenia participated in the study. All patients were treated with lithium carbonate, in addition to hepatoprotective drugs or drugs that increase white blood cell count. Thyroid function, liver function, and white blood cells were measured. Clinical outcomes were observed after a 1-year follow-up.Results. After treatment for 36 weeks, symptoms of hyperthyroidism and the level of thyroid hormones were improved and liver function, and white blood cells returned to a normal level. Twelve patients (23.5%) obtained clinical remission, 6 patients (11.8%) relapsed after withdrawal, 25 patients (49.0%) received radioiodine therapy, and 8 patients (15.7%) underwent surgical procedures after lithium carbonate treatment.Conclusion. Lithium carbonate has effects on the treatment of mild-to-moderate hyperthyroidism caused by GD, and it is particularly suitable for patients with ATD-induced hepatic injury or leukopenia.

scholarly journals The utility of basic blood counts, WBC histogram and C-reactive protein in detecting malaria

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jun Nishimura ◽  
Parag Dharap ◽  
Sebastien Raimbault
Keyword(s):  
Blood Cells ◽  
Malaria Infection ◽  
Monsoon Season ◽  
Characteristic Curve ◽  
White Blood Cells ◽  
C Reactive Protein ◽  
Control Groups ◽  
Reactive Protein ◽  
Hematology Analyzer ◽  
And Control

Abstract Background Hematology analyzers display abnormal parameters during malaria infection providing insightful information for suspecting and assessing malaria infection. The goal of this study is to demonstrate the potential of a three-part differential hematology analyzer to assess malaria, provide information about the parasitemia, and discuss the importance of combining C-reactive protein (CRP) with hematology parameters to obtain further information about the malaria infection. Methods The present study shows the results of a case–control study during the monsoon season of years 2018 and 2019 in Mumbai, India. The study considers 1008 non-malaria febrile cases, 209 P. vivax and 31 P. falciparum positive malaria samples, five cases of mixed P. vivax and P. falciparum infection, and three co-infection cases of P. vivax and dengue. Raw data from the three-part analyzer LC-667G CRP (HORIBA) and the corresponding microscopic findings (golden standard for diagnosis of malaria) were obtained for each sample. Results The medians of platelet counts (PLT) were 102.5, 109.0, and 223.0 × 103/µL, while CRP medians were 67.4, 81.4 and 10.4 mg/L in P. vivax, P. falciparum and control groups respectively (p < 0.001 in Mann–Whitney U tests between malaria and control groups). Compared with negative samples, platelets counting less than 161.5 × 103/µL were observed on malaria patients (OR 19.12, 95% CI 11.89–30.75). Especially in P. vivax cases, an abnormal peak was frequently observed in the white blood cells (WBC) histogram around the 37fL channel. The events counted around that channel showed a linear correlation with the counting of red blood cells infected predominantly with larger parasitic forms. Parameters like CRP (rs = 0.325, p < 0.001), WBC (rs = 0.285, p < 0.001) and PLT (rs = − 0.303, p < 0.001) were correlated with the parasitemia of P. vivax samples. Between the malaria and dengue groups, the highest area under the receiver operating characteristic curve was observed on CRP (0.867, CRP ≥ 26.85 mg/L). Conclusions A three-part differential hematology analyzer has the potential to not only trigger malaria diagnosis confirmation but also assess the severity of the infection when CRP is considered.

scholarly journals Risk Factors of White Blood Cell Progression Among Patients With Chronic Lymphocytic Leukemia at Felege Hiwot Referral Hospital, Bahir Dar, Ethiopia

2022 ◽  
Vol 21 ◽  
pp. 117693512110699
Author(s):  
Gedam Derbew Addisia ◽  
Awoke Seyoum Tegegne ◽  
Denekew Bitew Belay ◽  
Mitiku Wale Muluneh ◽  
Mahider Abere Kassaw
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Blood Cell ◽  
White Blood Cell ◽  
Blood Cells ◽  
White Blood Cells ◽  
Lymphocytic Leukemia ◽  
Bahir Dar ◽  
Felege Hiwot Referral Hospital ◽  
Wbc Count

Background: Leukemia is a type of cancers that start in the bone marrow and produce a serious number of abnormal white blood cells. Bleeding and bruising problems, fatigue, fever, and an increased risk of infection are among symptoms of the disease. The main objective of this study is to identify the determinant of the progression rate of white blood cells among patients with chronic lymphocytic leukemia at Felege Hiwot Referral Hospital (FHRH), Bahir Dar, Ethiopia. Methods: A retrospective study design was conducted on 312 patients with chronic lymphocytic leukemia at FHRH, Bahir Dar, Ethiopia under treatment from 1 January 2017 to 31 December 2019. A linear mixed-effects model was considered for the progression of the white blood cell data. Results: The estimated coefficient of the fixed effect intercept was 84.68, indicating that the average white blood cell (WBC) count of the patients was 84.68 at baseline time by excluding all covariates in the model ( P-value <.001). Male sex ( β = 2.92, 95% confidence interval [CI] 0.58, 0.5.25), age ( β = .17, 95% CI 0.08, 0.28), widowed/divorced marital status ( β = 3.30, 95% CI 0.03, 6.57), medium chronic lymphocytic leukemia (CLL) stage ( β = −4.34, 95% CI −6.57, −2.68), high CLL stage ( β = −2.76, 95% CI −4.86, −0.67), hemoglobin ( β = .15, 95% CI 0.07, 0.22), platelet ( β = .09, 95% CI 0.02, 0.17), lymphocytes ( β = .16, 95% CI 0.03, 0.29), red blood cell (RBC) ( β = .17, 95% CI 0.09, 0.25), and follow-up time ( β = .27, 95% CI 0.19, 0.36) were significantly associated with the average WBC count of chronic lymphocytic leukemia patients. Conclusions: The finding showed that age, sex, lymphocytic, stage of chronic lymphocytic leukemia, marital status, platelet, hemoglobin, RBC, and follow-up time were significantly associated with the average WBC count of chronic lymphocytic leukemia patients. Therefore, health care providers should give due attention and prioritize those identified factors and give frequent counseling about improving the health of chronic lymphocytic leukemia patients.

Targeting Survivin Using ICG-001 May Overcome Drug Resistance in Primary B-Cell Acute Lymphoblastic Leukemia.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 3072-3072
Author(s):  
Enzi Jiang ◽  
Eugene Park ◽  
Carlton Scharman ◽  
Yao-Te Hsieh ◽  
Asha Kadavallore ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Blood Cells ◽  
Lymphoblastic Leukemia ◽  
White Blood Cells ◽  
Leukemia Cells ◽  
Control Group ◽  
Drug Resistant ◽  
Control Groups ◽  
Standard Chemotherapy

Abstract Abstract 3072 Poster Board III-9 Despite advances in chemotherapeutic treatment of acute lymphoblastic leukemia (ALL), 20% of children relapse with high death rates, highlighting the need for new treatment modalities. Recent population studies have demonstrated that Survivin, a member of the inhibitor of apoptosis (IAP) family proteins, is expressed in most cancerous cells but has also been implicated in normal erythropoiesis. It is upregulated in ALL of relapsed patients but not in drug-sensitive ALL. The expression of Survivin depends on the formation of a complex between β-catenin and its co-activator CBP. Selective suppression of CBP/β-catenin signaling using the novel small-molecule inhibitor ICG-001 offers a novel mechanism to target Survivin in the sensitization of leukemia cells to conventional drug treatment. We hypothesize that inhibition of CBP/β-catenin signaling by ICG-001 in combination with conventional therapy represents a promising therapeutic principle to eradicate drug resistant ALL while sparing normal hematopoiesis. An in vivo study utilized our bioluminescent model to non-invasively monitor leukemogenesis of a primary ALL, transduced with a lentiviral construct encoding firefly luciferase prior to xenotransplantation. NOD/SCIDIL2R gamma-/- mice were sublethally irradiated prior intravenous injection of 50,000 cells per animal. Leukemic animals were treated with a combination of intraperitoneally administered VDL and ICG-001 (100mg/kg/d) (n=3), which was delivered via subcutaneous osmotic pumps to ensure stable plasma levels, with VDL only (n=4), or PBS only (n=2) as a control for 4 weeks. Bioluminescent imaging on Day 42 post-injection showed a contrast in the containment of leukemia of ICG-001+VDL mice as compared to those of the VDL control group. The animals in the PBS control group and the VDL+PBS Pump control groups had Median Survival Times (MST) of 35 days and 66.5 days post-treatment, respectively. In marked contrast, the animals treated with a combination of VDL+ICG-001 had a significant 14% extension in MST of 76 days post-treatment (p=0.016 compared to VDL group). Survivin mRNA expression was found to be downregulated after VDL+ICG treatment compared to treatment with VDL only. Analysis of peripheral blood showed no effect of ICG-001 on leukocyte or red blood cells compared to control groups. Next, we determined in vitro the ability of ICG-001 to increase sensitivity of patient-derived ALL cells and ALL celllines including BEL-1, REH, 697 and SUPB15 to chemotherapy including VDL or Imatinib. After 4 days we observed significantly increased toxicity assessed by MTT assay and AnnexinV staining as well as downregulation of Survivin confirmed by real-time PCR and Western Blot. To determine if ICG-001 is non-toxic to normal hematopoiesis, we treated normalC57BL/6 mice for 3 weeks with ICG-001 only. At end of treatment, normal blood counts including red blood cell, white blood cells and platelets, normal histology and normal weight gain indicated that ICG-001 is not detrimental to the recipient. In vitro apoptotic studies using normal white blood cells isolated from peripheral blood and co-cultured with a stromal layer confirmed further the non-toxicity of ICG-001 to normal cells. In summary, the sustained survival of the mice treated with combination of standard chemotherapy and ICG-001 is compatible with our hypothesis that ICG-001 can sensitize drug resistant leukemia cells to treatment with standard chemotherapy while sparing normal hematopoiesis and may lead to novel therapeutic options to overcome drug resistance. Disclosures No relevant conflicts of interest to declare.

scholarly journals In Vivo Screening and Antidiabetic Potential of Polyphenol Extracts from Guava Pulp, Seeds and Leaves

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1714
Author(s):  
Hassan Shabbir ◽  
Tusneem Kausar ◽  
Sobia Noreen ◽  
Hafeez ur Rehman ◽  
Ashiq Hussain ◽  
...  
Keyword(s):  
Feed Intake ◽  
Blood Cells ◽  
White Blood Cells ◽  
Radical Scavenging ◽  
Density Lipoprotein ◽  
Total Phenolic ◽  
Control Group ◽  
Albino Rats ◽  
Control Groups

The present study investigates the antidiabetic potential of polyphenol extracts purified from guava pulp, seeds and leaves using an in vivo experiment on albino rats. The polyphenols from guava pulp, seeds and leaves were extracted using methanol solvent and the sonication method while being evaluated by total phenolic contents and radical scavenging activity assay. The proximate composition of powders revealed that ash, protein and total sugars were significantly (p < 0.05) higher in leaves and seeds, while vitamin C was highest in pulp. Total phenolic and antioxidant activities were highest in pulp followed by leaves and seeds. The findings of feed intake and body gain revealed that the supplementation of polyphenols, especially from pulp, significantly (p < 0.05) increased the feed intake, which resulted in increased body weight. Moreover, total cholesterol (TC) and low-density lipoprotein (LDL) levels were significantly (p < 0.05) decreased, while the level of high-density lipoprotein (HDL) was increased in groups fed with polyphenols from guava pulp compared to both (+ive and –ive) control groups. Furthermore, blood glucose and triglycerides were significantly (p < 0.05) decreased in supplemented groups compared to the control group of diabetes mice, which resulted in the inhibition of α-amylase and glucose transport. Besides this, packed cell volume (PCV), mean corpuscular volume (MCV), hemoglobin, red blood cells (RBCs), white blood cells (WBCs) and platelet levels were increased significantly (p < 0.05) in pulp’s extract followed by leaves and seeds compared to both control groups. Overall, the antidiabetic potential of different extracts was in the following order: pulp > leaves > seeds. The findings suggest the feasibility of adding 200–250 mg/kg.bw of polyphenol extracts of pulp as an alternative to diabetic drugs.

Atypical cells in sysmex UN automated urine particle analyzer: a case report and pitfalls for future studies

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Ozgur Aydin ◽  
Onur Yapıcı ◽  
Ruksan Copuroglu
Keyword(s):  
Case Report ◽  
Blood Cells ◽  
White Blood Cells ◽  
Pathology Report ◽  
Case Presentation ◽  
Future Studies ◽  
Atypical Cells ◽  
Papillary Lesions ◽  
Automated Instrument

AbstractObjectivesSysmex UN series fully automated urine analyzer reports a series of research parameters besides routine parameters including the “atypical cells” parameter. An automated instrument in clinical use capable of detecting neoplastic cells of the urinary tract will have paramount significance.Case presentationA 73 years old male patient with a recurrent high grade urothelial carcinoma admitted to our urology outpatient clinic due to hematuria.Urinalysis showed +3 hemoglobin, +3 leukocyte esterase, 200/HPF red blood cells, 300/HPF white blood cells. The instrument also reported atypical cells (7.6/µL or 1.3/HPF) under the heading of “research parameters.” Presence of atypical cells was confirmed by the manual microscopy. The patient has undergone transurethral resection of papillary lesions and the pathology report confirmed a recurrence. On follow-up, atypical cells fell to 0.1/µL after 40 days.ConclusionsThis case report presents a patient with atypical cells in urine, detected by a fully automated urine analyzer. The atypical cells presented on the screen of the analyzer were confirmed by the manual microscopy. This presentation may influence future studies pertaining to the subject.

scholarly journals Effect of Vitamin A and C on some physiological parameters of rabbits in Iraq

2014 ◽  
Vol 8 (3) ◽  
pp. 23-27
Author(s):  
Sabah A.A. Rahman ◽  
Riyad M.R. Al-Mashat
Keyword(s):  
Vitamin A ◽  
Vitamin C ◽  
Blood Cells ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
The Other ◽  
Control Groups ◽  
Fourth Group ◽  
Average Body ◽  
Average Body Weight

Twenty four male rabbits were used in this experiments 6 weeks of age with average of 700-750 g, divided in to four equal groups (6 rabbits each). Animals of second group was equally injected with vitamin A, animals third group injection with 700 mg/kg vitamin C, animals fourth group injection with 3000 IU vitamin A + 700 mg vitamin C and animals of first group was the control groups. Every two week were weight, noticed increased in weight but not significant in the average body weight of the rabbits that injected with vitamin A from the other groups, but when we tacked the blood plasma from the rabbits after six weeks from the beginning of the experiments noticed significant increased p<0.05 in white blood cells number (6.20±0.69), hemoglobin concentration (12.11±0.01), red blood cells (4.43±0.03), platelets (305.6±12.91). however, decrease in packed cell volume (38.51±0.28) in animals groups injected by mixed vitamin A&C. Although, noticed non-significant increased in total protein (6.29±0.13), globulin (2.66±0.26) in animals were injected with vitamin A&C increased in average albumin, and decreased in globulin in animals were injected with vitamin A, however, increase in SAST & SALT enzymes, creatinine (1.37±0.16) and urea (40.34±1.15) in animals groups injected with vitamin A, also increase non significant in cholesterol (98.43±2.82) and total lipid ( 14.96±1.82) in animals were injected with vitamin A and C.

scholarly journals Effects of diet supplementation with different level of Celmanax® in rainbow trout (Oncorhyncus mykiss)

2019 ◽  
Vol 70 (1) ◽  
pp. 1347
Author(s):  
A. KHODADADI ◽  
A. HAGHIGHI ◽  
H. MALEKINEJADH ◽  
A. TUKMECHI ◽  
M. AFSHARNASAB
Keyword(s):  
Rainbow Trout ◽  
Neutrophil Count ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Control Groups ◽  
Serum Biochemical ◽  
And Control

The aim of this study was to evaluate the effect of a prebiotic (Celmanax®) containing yeast cell wall with mannan oligosaccharides on the haematological and serum biochemical parameters in rainbow trout. Three levels of prebiotic (0, 0.1, 0.5 and 1 %) were mixed into pellets. Fish (19.08±1.45 g) were fed a supplemented commercial diet for 60 days. Blood samples were colected from the onset and on days 30 and 60 of the trial to measure the haematological and serum biochemical parameters in rainbow trout. The results showed significant differences in haemoglobin, mean corpuscular volume,mean corpuscular haemoglobin concentration, white blood cells and neutrophil count between control and all test groups (p<0.05). The highest and the lowest white blood cells and neutrophil count (on day 60) were observed in the 0.1 %, prebiotic-received and control groups, respectively. Also, the result showed significant differences in Alkaline phosphatase enzymes, serum glutamic oxaloacetic transaminase, Serum glutamic-pyruvic transaminase, between the test and control groups(p<0.05) while non-significant elevation of blood urea nitrogen, creatinine and total protein levels was found in the Celmanax®-received groups (p>0.05). These results suggest that the Celmanax® supplementation enhances white blood cells and neutrophil count, and changes some biochemical parameters in rainbow trout.

scholarly journals Normal range of white blood cells and differential count of Sudanese in Khartoum state

2018 ◽  
Vol 5 (4) ◽  
pp. 784 ◽  
Author(s):  
Elmutaz H. Taha ◽  
Mohammed Elshiekh ◽  
Abdelrahim Alborai ◽  
Elnagi Y. Hajo ◽  
Abdelmohisen Hussein ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Differential Count ◽  
Reference Ranges ◽  
African Countries ◽  
The Mean ◽  
Automated Hematology Analyzer ◽  
Wbc Count ◽  
Age Range

Background: The normal physiological range for white blood cells and differential count are essential for diagnosis, treatment, follow up and screening. This study aimed at establishing the reference ranges of WBCs and differential count in Sudanese people.Methods: The present study included 444 healthy adult Sudanese from both sexes with age range of 20 – 60 years. Blood samples were obtained from brachial veins and drawn in EDTA tubes. WBCs and differential count were analyzed using Sysmex KX-21 automated hematology analyzer. Full clinical examination was performed, weight and height were measured, and BMI was calculated.Results: The mean WBC count was 5.1±1.5×103/ µl with a range of 3.6 ×103/µl to 6.6 ×103/µl. The mean WBCs count for males and females were 4.969×103/µl and 5.138×103/µl respectively. Neutrophils count was 2.430×103/µl (47%) and mean for lymphocyte count was 2.116×103/µl (41.1%).Conclusions: WBCs count was directly proportional to BMI. The WBCs count of Sudanese people was lower than that of Caucasians and similar to reports from other African countries.

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