scholarly journals Production of fungal volatile organic compounds in bedding materials

1997 ◽  
Vol 6 (3) ◽  
pp. 219-227 ◽  
Author(s):  
Saana Lappalainen ◽  
Anna-Liisa Pasanen ◽  
Pentti Pasanen ◽  
Pentti Kalliokoski
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Laboratory Experiments ◽  
Fungal Growth ◽  
Co2 Production ◽  
Growth Media ◽  
Emission Rates ◽  
Potential Growth ◽  
Volatile Organic ◽  
Bedding Materials

The high relative humidity of the air and many potential growth media, such as bedding materials, hay and grains in the horse stable, for example, provide suitable conditions for fungal growth. Metabolic activity of four common agricultural fungi incubated in peat and wood shavings at 25°C and 4°C was characterized in this study using previously specified volatile metabolites of micro-organisms and CO2 production as indicators. The volatile organic compounds were collected into Tenax resin and analysed by gas chromatography. Several microbial volatile organic compounds (MVOCs), e.g. 1-butanol, 2-hexanone, 2-heptanone, 3-octanone, 1-octen-3-ol and 1-octanol were detected in laboratory experiments; however, these accounted for only 0.08-1.5% of total volatile organic compounds (TVOCs). Emission rates of MVOCs were 0,001-0.176 μg/kg of bedding materials per hour. Despite some limitations of the analytical method, certain individual MVOCs, 2-hexanone, 2-heptanone and 3-octanone, were also detected in concentrations of less than 4.6 μg/m3 (0.07-0.31% of TVOC) in a horse stable where peat and shavings were used as bedding materials. MVOC emission rate was estimated to be 0.2-2.0 μg/kg x h-1 from bedding materials in the stable, being about ten times higher than the rates found in the laboratory experiments. Some compounds, e.g. 3-octanone and 1-octen-3-ol, can be assumed to originate mainly from microbial metabolisms.

scholarly journals Trichoderma asperellum T76-14 Released Volatile Organic Compounds against Postharvest Fruit Rot in Muskmelons (Cucumis melo) Caused by Fusarium incarnatum

2021 ◽  
Vol 7 (1) ◽  
pp. 46
Author(s):  
Warin Intana ◽  
Suchawadee Kheawleng ◽  
Anurag Sunpapao
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Cucumis Melo ◽  
Fungal Growth ◽  
Fruit Rot ◽  
Postharvest Disease ◽  
Dual Culture ◽  
Trichoderma Asperellum ◽  
Volatile Organic

Postharvest fruit rot caused by Fusarium incarnatum is a destructive postharvest disease of muskmelon (Cucumis melo). Biocontrol by antagonistic microorganisms is considered an alternative to synthetic fungicide application. The aim of this study was to investigate the mechanisms of action involved in the biocontrol of postharvest fruit rot in muskmelons by Trichoderma species. Seven Trichoderma spp. isolates were selected for in vitro testing against F. incarnatum in potato dextrose agar (PDA) by dual culture assay. In other relevant works, Trichoderma asperellum T76-14 showed a significantly higher percentage of inhibition (81%) than other isolates. Through the sealed plate method, volatile organic compounds (VOCs) emitted from T. asperellum T76-14 proved effective at inhibiting the fungal growth of F. incarnatum by 62.5%. Solid-phase microextraction GC/MS analysis revealed several VOCs emitted from T. asperellum T76-14, whereas the dominant compound was tentatively identified as phenylethyl alcohol (PEA). We have tested commercial volatile (PEA) against in vitro growth of F. incarnatum; the result showed PEA at a concentration of 1.5 mg mL−1 suppressed fungal growth with 56% inhibition. Both VOCs and PEA caused abnormal changes in the fungal mycelia. In vivo testing showed that the lesion size of muskmelons exposed to VOCs from T. asperellum T76-14 was significantly smaller than that of the control. Muskmelons exposed to VOCs from T. asperellum T76-14 showed no fruit rot after incubation at seven days compared to fruit rot in the control. This study demonstrated the ability of T. asperellum T76-14 to produce volatile antifungal compounds, showing that it can be a major mechanism involved in and responsible for the successful inhibition of F. incarnatum and control of postharvest fruit rot in muskmelons.

Pathogen suppression by microbial volatile organic compounds in soils

2019 ◽  
Vol 95 (8) ◽  
Author(s):  
Wietse de Boer ◽  
Xiaogang Li ◽  
Annelein Meisner ◽  
Paolina Garbeva
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Abiotic Factors ◽  
Management Strategies ◽  
Field Studies ◽  
Growth Media ◽  
Soil Borne Pathogens ◽  
Volatile Organic ◽  
Microbial Volatile Organic Compounds ◽  
Pathogen Suppression

ABSTRACT There is increasing evidence that microbial volatile organic compounds (mVOCs) play an important role in interactions between microbes in soils. In this minireview, we zoom in on the possible role of mVOCs in the suppression of plant-pathogenic soil fungi. In particular, we have screened the literature to see what the actual evidence is that mVOCs in soil atmospheres can contribute to pathogen suppression. Furthermore, we discuss biotic and abiotic factors that influence the production of suppressive mVOCs in soils. Since microbes producing mVOCs in soils are part of microbial communities, community ecological aspects such as diversity and assembly play an important role in the composition of produced mVOC blends. These aspects have not received much attention so far. In addition, the fluctuating abiotic conditions in soils, such as changing moisture contents, influence mVOC production and activity. The biotic and abiotic complexity of the soil environment hampers the extrapolation of the production and suppressing activity of mVOCs by microbial isolates on artificial growth media. Yet, several pathogen suppressive mVOCs produced by pure cultures do also occur in soil atmospheres. Therefore, an integration of lab and field studies on the production of mVOCs is needed to understand and predict the composition and dynamics of mVOCs in soil atmospheres. This knowledge, together with the knowledge of the chemistry and physical behaviour of mVOCs in soils, forms the basis for the development of sustainable management strategies to enhance the natural control of soil-borne pathogens with mVOCs. Possibilities for the mVOC-based control of soil-borne pathogens are discussed.

scholarly journals Yeast Smell Like What They Eat: Analysis of Volatile Organic Compounds of Malassezia furfur in Growth Media Supplemented with Different Lipids

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 419 ◽  
Author(s):  
Mabel Gonzalez ◽  
Adriana Celis ◽  
Marcela Guevara-Suarez ◽  
Jorge Molina ◽  
Chiara Carazzone
Keyword(s):  
Oleic Acid ◽  
Volatile Organic Compounds ◽  
Palmitic Acid ◽  
Organic Compounds ◽  
Future Research ◽  
Growth Media ◽  
Unsupervised Hierarchical Cluster ◽  
Malassezia Furfur ◽  
Volatile Organic ◽  
Latent Structures

Malassezia furfur is part of the human skin microbiota. Its volatile organic compounds (VOCs) possibly contribute to the characteristic odour in humans, as well as to microbiota interaction. The aim of this study was to investigate how the lipid composition of the liquid medium influences the production of VOCs. Growth was performed in four media: (1) mDixon, (2) oleic acid (OA), (3) oleic acid + palmitic acid (OA+PA), and (4) palmitic acid (PA). The profiles of the VOCs were characterized by HS-SPME/GC-MS in the exponential and stationary phases. A total number of 61 VOCs was found in M. furfur, among which alkanes, alcohols, ketones, and furanic compounds were the most abundant. Some compounds previously reported for Malassezia (γ-dodecalactone, 3-methylbutan-1-ol, and hexan-1-ol) were also found. Through our experiments, using univariate and multivariate unsupervised (Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA)) and supervised (Projection to Latent Structures Discriminant Analysis (PLS-DA)) statistical techniques, we have proven that each tested growth medium stimulates the production of a different volatiles profile in M. furfur. Carbon dioxide, hexan-1-ol, pentyl acetate, isomer5 of methyldecane, dimethyl sulphide, undec-5-ene, isomer2 of methylundecane, isomer1 of methyldecane, and 2-methyltetrahydrofuran were established as differentiating compounds among treatments by all the techniques. The significance of our findings deserves future research to investigate if certain volatile profiles could be related to the beneficial or pathogenic role of this yeast.

scholarly journals Fluxes and concentrations of volatile organic compounds from a South-East Asian tropical rainforest

2010 ◽  
Vol 10 (17) ◽  
pp. 8391-8412 ◽  
Author(s):  
B. Langford ◽  
P. K. Misztal ◽  
E. Nemitz ◽  
B. Davison ◽  
C. Helfter ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Tropical Forests ◽  
Atmospheric Chemistry ◽  
Carbon Flux ◽  
Tropical Rainforest ◽  
East Asian ◽  
Emission Rates ◽  
Reactive Carbon ◽  
Volatile Organic

Abstract. As part of the OP3 field study of rainforest atmospheric chemistry, above-canopy fluxes of isoprene, monoterpenes and oxygenated volatile organic compounds were made by virtual disjunct eddy covariance from a South-East Asian tropical rainforest in Malaysia. Approximately 500 hours of flux data were collected over 48 days in April–May and June–July 2008. Isoprene was the dominant non-methane hydrocarbon emitted from the forest, accounting for 80% (as carbon) of the measured emission of reactive carbon fluxes. Total monoterpene emissions accounted for 18% of the measured reactive carbon flux. There was no evidence for nocturnal monoterpene emissions and during the day their flux rate was dependent on both light and temperature. The oxygenated compounds, including methanol, acetone and acetaldehyde, contributed less than 2% of the total measured reactive carbon flux. The sum of the VOC fluxes measured represents a 0.4% loss of daytime assimilated carbon by the canopy, but atmospheric chemistry box modelling suggests that most (90%) of this reactive carbon is returned back to the canopy by wet and dry deposition following chemical transformation. The emission rates of isoprene and monoterpenes, normalised to 30 °C and 1000 μmol m−2 s−1 PAR, were 1.6 mg m−2 h−1 and 0.46mg m−2 h−1 respectively, which was 4 and 1.8 times lower respectively than the default value for tropical forests in the widely-used MEGAN model of biogenic VOC emissions. This highlights the need for more direct canopy-scale flux measurements of VOCs from the world's tropical forests.

Selected problems concerning volatile organic compounds emission reduction from thick-veneer pine plywood

2020 ◽  
Vol 111 ◽  
pp. 131-20
Author(s):  
Martyna Dyrwal ◽  
Piotr Borysiuk
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Carbonyl Compounds ◽  
Emission Reduction ◽  
Caproic Acid ◽  
Emission Rates ◽  
Wood Material ◽  
Soaking Time ◽  
Volatile Organic ◽  
Higher Temperature

Selected problems concerning volatile organic compounds emission reduction from thick-veneer pine plywood. The paper presents results of research conducted on volatile organic compounds emissions, considering two different production parameter sets for thick-veneer pine plywood, manufactured in industrial conditions. Both types of plywood were produced from raw wood material, which was hydrothermally treated under two different variants of parameters (I – 47˚C, 19 h; II – 55˚C, 24 h). Based on the results it was stated that severe hydrothermal treatment of raw wood material (longer soaking time, higher temperature) had impact on reduction of plywood VOC emission rates. Main VOCs emitted from pine plywood were monoterpenes and carbonyl compounds. Of the monoterpenes, α-pinene and 3-carene had the highest emission. Of the carbonyl group of compounds, the highest emission had hexanal and caproic acid.

First Comprehensive Measurements of Emission Rates of Greenhouse Gases, Volatile Organic Compounds and Reduced Sulfur Compounds from a Tailings Pond in the Alberta Oil Sands

2020 ◽  
Author(s):  
Ralf Staebler ◽  
Samar Moussa ◽  
Yuan You ◽  
Hayley Hung ◽  
Maryam Moradi ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Oil Sands ◽  
Regulatory Compliance ◽  
Sulfur Compounds ◽  
Emission Rates ◽  
Reduced Sulfur Compounds ◽  
Volatile Organic ◽  
Reduced Sulfur

<p>Canada’s Oil Sands Region in northern Alberta contains the world’s largest deposits of commercially exploited bitumen. Extraction of synthetic crude oil from these deposits is a water intensive process, requiring large ponds for water recycling and/or final storage of tailings, already covering a total of over 100 km<sup>2</sup> of liquid surface area in the Athabasca Oil sands. The primary extraction tailings ponds primarily contain sand, silt, clay and unrecovered bitumen, while a few secondary extraction ponds also receive solvents and inorganic and organic by-products of the extraction process. Fugitive emissions of pollutants from these ponds to the atmosphere may therefore be a concern, but until recently, data on emission rates for many pollutants, other than a few reported under regulatory compliance monitoring, were sparse. We present here the results from a comprehensive field campaign to quantify the emissions from a secondary extraction pond to the atmosphere of 68 volatile organic compounds (VOCs), 22 polycyclic aromatic compounds (PACs), 8 reduced sulfur compounds as well as methane, carbon dioxide and ammonia. Three micrometeorological flux methods (eddy covariance, vertical gradients and inverse dispersion modeling) were evaluated for methane fluxes to ensure their mutual comparability. Methane and carbon dioxide fluxes were similar to previous results based on flux chamber measurements. Emission rates for 12 PACs, alkanes and aromatic VOCs, several sulfur species, and ammonia were found to be significant. PACs were dominated by methyl naphthalenes and phenanthrenes, while diethylsulfide and  and n-heptane were the dominant reduced sulfur and VOC species, respectively. The role of these previously unavailable emission rates in regional pollutant budgets will be discussed.</p>

scholarly journals Effect of volatile organic compounds mediated fungal growth inhibition by Trichoderma asperellum HbGT6-07

2021 ◽  
Author(s):  
Md Kamaruzzaman ◽  
Md. Samiul Islam ◽  
Shakil Ahmed Polash ◽  
Razia Sultana
Keyword(s):  
Volatile Organic Compounds ◽  
Growth Inhibition ◽  
Botrytis Cinerea ◽  
Organic Compounds ◽  
Sclerotinia Sclerotiorum ◽  
Mycelial Growth ◽  
Fungal Growth ◽  
Trichoderma Asperellum ◽  
Volatile Organic ◽  
Fungal Growth Inhibition

Abstract The species of Trichoderma are one of the most frequently used natural biocontrol agents to mitigate plant diseases and improve crop yields. In this study, sixteen Trichoderma spp. were isolated from soil of different regions of China. However, we identified Trichoderma. asperellum HbGT6-07 by initial fungal growth inhibition assay and molecular approach and also evaluated the antimicrobial effects. Tested 10% concentrated culture filtrate of T. asperellum HbGT6-07 inhibited 93 % of colony radial growth in Botrytis cinerea (B05.10) as well as 91 % of Sclerotinia sclerotiorum (A367). VOCs emitted from HbGT6-07 have antimicrobial properties against Botrytis cinerea (B05.10) and Sclerotinia sclerotiorum (A367). In in-vitro DwD method, The T. asperellum HbGT6-07 volatile organic compounds (VOCs) effectively reduced colonial diameter, mycelial growth rate and sclerotia production by two virulent fungal pathogens. The GC-MS analysis identified thirty-two VOCs derived from HbGT6-07 isolates. Moreover, the hyphal fragments of the T. asperellum HbGT6-07 demonstrated successful mycelia growth suppression of two virulent fungal agents by competing toward the invasion on oilseed rape leaves. The above findings indicated that T. asperellum HbGT6-07 could attain competitive progress via volatile antifungal compound production and comprehensive mycelial growth. This study provided an outlook of using T. asperellum HbGT6-07 to control virulent pathogens of B. cinerea and S. sclerotiorum.

scholarly journals Volatile Organic Compounds Emanating from Indoor Ornamental Plants

HortScience ◽  
2009 ◽  
Vol 44 (2) ◽  
pp. 396-400 ◽  
Author(s):  
Dong Sik Yang ◽  
Ki-Cheol Son ◽  
Stanley J. Kays
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Ornamental Plants ◽  
Growth Media ◽  
Diffusion Resistance ◽  
Plant Signaling ◽  
Volatile Organic ◽  
Broad Cross Section ◽  
Gas Chromatography Mass Spectroscopy ◽  
Micro Organisms

A broad cross-section of volatiles emanating from four species of popular indoor ornamental plants (Spathiphyllum wallisii Regel, Sansevieria trifasciata Prain, Ficus benjamina L., and Chrysalidocarpus lutescens Wendl.) was identified and categorized based on source. Volatile organic compounds from individual plants were obtained using a dynamic headspace system and trapped on Tenax TA during the day and again at night. Using short-path thermal desorption and cryofocusing, the volatiles were transferred onto a capillary column and analyzed using gas chromatography–mass spectroscopy. The volatiles originated from the plants, media/micro-organisms, pot, and pesticides. A total of 23, 12, 13, and 16 compounds were identified from S. wallisii, S. trifasciata, F. benjamina, and C. lutescens, respectively. The night emanation rate was substantially reduced (i.e., by 30.1%, 69.5%, 73.7%, and 63.1%, respectively) reflecting in part the regulation of biosynthesis and the greater diffusion resistance when the stomata were closed. S. wallisii had the highest emanation rate, releasing 15 terpenoid compounds [e.g., linaloloxide, linalool, (Z)-β-farnesene, farnesal, (+)-δ-cadinene, (+)-β-costol] into the surrounding air. Alpha-farnesene (90.3%) was quantitatively the dominant volatile present followed by (Z)-β-farnesene (1.4%), (+)-β-costol (1.4%), and farnesal (1.1%). Substantially fewer terpenoids (i.e., two, nine, and eight) emanated from S. trifasciata, F. benjamina, and C. lutescens, which quantitatively emitted fewer volatiles than S. wallisii. Most terpenoids from the four species were sesquiterpenes rather than monoterpenes. Methyl salicylate, a plant-signaling compound, was emitted by all four species. Certain volatiles (e.g., 2-chlorobenzonitrile, 1-ethyl-3,5-dimethylbenzene) were released from growth media and/or micro-organisms therein; other sources included the plastic pot (e.g., 2-ethyl-1-hexanol, octamethyl cyclotetrasiloxane) and pesticide ingredients [e.g., 2-(2-methoxy- ethoxy)ethanol, 2-ethylhexyl salicylate, homosalate].

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