scholarly journals An analytical and breeding study on fatty acids in summer turnip rape (brassica campestris L. var. annua)

1986 ◽  
Vol 58 (3) ◽  
pp. 103-141
Author(s):  
Into Laakso

The fatty acid composition of the seed oil of summer turnip rape (Brassica campestris L. var. annua) was investigated by gas liquid chromatography (GLC). The reliability of conventional sampling methods in capillary GC was compared with that of the new on column and PTV (programmed temperature vaporizer) techniques, with particular reference to the determination of fatty acid variation. In order to develop new, well-adapted turnip rape strains with improved oil quality, a breeding programme for a higher linoleic acid content, based on individual plant selection, was performed in 1978—85. The results showed that the conventional sampling techniques involving sample transfer to a hot injector were very unreliable as regards precision and accuracy. This was especially the case in the determination of trace fatty acid levels. The PTV methods with splitless and solvent split mode were as precise as cold on-column injection. The PTV sampling modifications, which are all superior to classical techniques, were even more suitable for routine analyses than on-column injection, where several restrictions are met. The analytical error with PTV for most of the compounds represented less than 1 % of the variation found for fatty acids within a turnip rape variety. The breeding experiments indicated that the level of linoleic acid can be increased under open-pollinated conditions in the field without affecting the α-linolenic acid content. The greenhouse conditions, on the other hand, were found to have a considerable environmental influence on the variation of these compounds, resulting in no response to linoleic-acid selection. In field trials, several strains with yields comparable to the varieties commonly cultivated in Finland were selected with a higher linoleic acid content (up to25 %). Most of them also contained no erucic acid. The new evidence concerning its beneficial physiological effects indicate that rapeseed oil should be considered as a serious alternative among sources of essential fatty acids. Such aspects should also be taken into account in future breeding of rapeseed fatty acids.

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Ali Karabacak ◽  
İbrahim Aytekin ◽  
Saim Boztepe

This study was conducted to compare fatty acid composition and content of conjugated linoleic acid (CLA) in different regions of sheep carcasses. Lambs of the Dağlıç breed were used for this purpose. Subsequent to a 68-day period of intensive fattening, fatty acids were examined in samples taken from the legs, shoulders, breasts, and ribs of lamb carcasses. According to the analysis, in leg, shoulder, breast, and rib, respectively, total saturated fatty acids (SFA) were found to be 40.38, 42.69, 42.56, and 40.27%, unsaturated fatty acids (MUFA) were found to be 40.38, 44.17, 46.17, and 49.50%, polyunsaturated fatty acids (PUFA) were found to be 4.79, 4.29, 3.80, and 3.72%, and CLAs were found to be 1.49, 1.69, 1.53, and 1.59%.


2002 ◽  
Vol 85 (1) ◽  
pp. 86-94 ◽  
Author(s):  
Subramaniam Satchithanandam ◽  
Jan Fritsche ◽  
Jeanne I Rader

Abstract Twelve powdered and 13 liquid infant formulas were analyzed by using an extension of AOAC Official Method 996.01 for fat analysis in cereal products. Samples were hydrolyzed with 8N HCl and extracted with ethyl and petroleum ethers. Fatty acid methyl esters were prepared by refluxing the mixed ether extracts with methanolic sodium hydroxide in the presence of 14% boron trifluoride in methanol. The extracts were analyzed by gas chromatography. In powdered formulas, saturated fatty acid (SFA) content (mean ± SD; n = 12) was 41.05 ± 3.94%, monounsaturated fatty acid (MUFA) content was 36.97 ± 3.38% polyunsaturated fatty acid (PUFA) content was 20.07 ± 3.08%, and total trans fatty acid content was 1.30 ± 1.27%. In liquid formulas, SFA content (mean ± SD; n = 13) was 42.29 ± 2.98%, MUFA content was 36.05 ± 2.47%, PUFA content was 20.65 ± 2.40%, and total trans fatty acid content was 0.88 ± 0.54%. Total fat content in powdered formulas ranged from 4.4 to 5.5 g/100 kcal and linoleic acid content ranged from 868 to 1166 mg/100 kcal. In liquid formulas, total fat content ranged from 4.1 to 5.1 g/100 kcal and linoleic acid content ranged from 820 to 1100 mg/100 kcal. There were no significant differences between powdered and liquid infant formulas in concentrations of total fat, SFA, MUFA, PUFA, or trans fatty acids.


2015 ◽  
Vol 66 (3) ◽  
pp. 254-259 ◽  
Author(s):  
Nadia Manzo ◽  
Fabiana Pizzolongo ◽  
Immacolata Montefusco ◽  
Maria Aponte ◽  
Giuseppe Blaiotta ◽  
...  

1987 ◽  
Vol 14 (1) ◽  
pp. 7-11 ◽  
Author(s):  
A. J. Norden ◽  
D. W. Gorbet ◽  
D. A. Knauft ◽  
C. T. Young

Abstract The improvement of peanut (Arachis hypogaea L.) oil quality has long been an objective of the Florida breeding program, since it influences the shelf-life and nutritional quality of manufactured products. Fatty acid distribution of the peanut genotypes (228 in 1984 and 298 in 1985) from the Gainesville and Marianna locations was determined by gas-liquid chromatography. A wider range in fatty acid composition, especially in oleic and linoleic acids, was found among these genotypes than that reported previously in the literature for the cultivated peanut. Two closely related experimental lines (435–2–1 and 435–2–2) had 80% oleic and 2% linoleic acid, with iodine values of 74. For the Florida breeding lines, iodine values of the oil ranged from 74 to 107 and the oleic/linoleic (O/L) ratios from 0.9 to 35:1. Florunner, by comparison, has an iodine value of 95 and an O/L ratio of slightly less than 2. The oleic acid content of the different experimental lines ranged from 37% to 80%, and the linoleic acid content from 2% to 43%. The magnitude of this variability permits the development of peanut cultivars with a range of oil composition for improved nutritional and industrial purposes. All the oil quality factors were highly significantly affected by genotype, and all but three of the factors were significantly affected by season.


1988 ◽  
Vol 28 (5) ◽  
pp. 629 ◽  
Author(s):  
DL George ◽  
CM McLeod ◽  
BW Simpson

Variation among F3 seeds from a single head for linoleic acid content was high (50.0-72.7%) and appeared unrelated to seed position. Fatty acid content of seeds within self-pollinated heads was not affected by unfilled, adjacent seeds. Thus single seed selections for linoleic acid content should not be biased. No general relationship between fatty acid level and seed position was evident from, analyses of bulk samples except that palmitic acid content of seeds from the upper half of heads (7.4%) was higher than from the lower half (6.5%). This may be related to the negative correlation (r = -0.45) between palmitic acid content and seed size. Fatty acid content and weight of seeds from the lower half of heads were less variable than from the upper half.


2018 ◽  
Vol 2 (1) ◽  
pp. 24 ◽  
Author(s):  
Eka Sariningsih ◽  
Mia Srimiati

<p><em>Avocado is a fruit that high unsaturated fatty aci</em><em>d, while</em><em> coconut water is fluid </em><em>which has</em><em> high potassium. </em><em>The objective of this study was<strong> </strong>t</em><em>o assess the formulation of avocado juice with coconut water that could potentially reduce hypertension. The design of the study was an experimental with the degree of subsititution of coconut water as treatments. There were 3 kinds of treatment, i.e. F1 (75% avocado and 25% coconut water), F2 (50% avocado and 50% coconut water), F3 (25% avocado and 75% coconut water) and F0 (without coconut water but using water). Avocado juice product with 75% avocado substitution and 25% coconut water (F1) was a best formula determined by the hedonic test. This formula contained 68,48% (%w/w) water, 3,12% (%w/w) ash content, 4,45% (%w/w) fat content, 1,76% (%w/w) protein content, 21,07% (%w/w) carbohydrate content, 2163,8 (mg/kg) potassium content, 9,20% (%w/w) linoleic acid content, 1,11% (%w/w) linolenic acid content and 42,4% (%w/w) of total unsaturated fatty acid. This product could potentially reduce and prevent hypertension, because in one serving size (200cc) contain 432,76 mg of potassium (claim source of potassium). </em><em></em></p>


2016 ◽  
Vol 69 (4) ◽  
Author(s):  
Józef Klocek ◽  
Magdalena Szwed ◽  
Danuta Koczkodaj ◽  
Joanna Mitrus ◽  
Marian Saniewski ◽  
...  

The effect of methyl jasmonate (MJ) vapors on the composition and peroxidation of major fatty acids in the organs of common buckwheat seedlings was investigated. The composition of fatty acids in the hypocotyl and cotyledons of seedlings changed significantly under exposure to MJ vapors in closed jars. Four-day exposure to MJ led to a significant reduction in the concentrations of stearic, linoleic, and linolenic acids in the hypocotyl, whereas oleic acid levels increased approximately 3.5-fold. A decrease in stearic acid levels and an increase in the content of linolenic acid were noted in cotyledons, whereas oleic acid levels decreased in roots. Seven-day exposure to MJ vapor caused a further reduction in stearic acid content and an increase in oleic acid and linoleic acid levels in the hypocotyl. At the same time, the linoleic acid content of roots and linolenic acid levels in cotyledons were doubled, but a 5-fold reduction in linolenic acid concentrations was observed in roots. Methyl jasmonate intensified fatty acid peroxidation in cotyledons after 4 and 7 days and in roots after 4 days of exposure. Peroxidation was inhibited in the hypocotyl and roots after 7 days. The noted changes in the composition and peroxidation of fatty acids are probably indicative of senescence in buckwheat seedlings under the influence of MJ. Senescence seems to proceed faster in cotyledons than in other organs of buckwheat seedlings.


2008 ◽  
pp. 27-32
Author(s):  
Anikó Borosné Győri ◽  
Istvánné Hermán ◽  
János Csapó ◽  
János Gundel

The composition of fatty acids in food products is a significant factor in human health. Feeding can significantly influence the composition of fatty acids in the animal fat. We analysed the effect of feeding high CLA-content (conjugated linoleic acid) feed on the composition of fatty acids in pork. The animals were grouped according to the following: Group 1) feeding experimental, ghee-mixed feed for 76 days, Group 2) feeding the same feed, but only for 33 days, Group 3) feeding sunflower-oil-mixed feed for 76 days. Ghee contains CLA in high amount. The aim of our experiment is to analyse how the high CLA content influences the fatty acid content of pork. In the end of the fattening experiment the animals were slaughtered, then samples were taken from the loin, ham, abdomen and backfat from 10 animals from each group and analysed the fatty acid content. We found significant differences between the average fatty acid content of the samples. As an effect of feeding ghee-enriched feed, the CLA content significantly increased, compared to the control group. However, the linoleic acid and the arachidonic acid content were lower, and the proportion of fatty acids was also lower when feeding control feed.


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