scholarly journals Polyol mixture supplementation in the diet of breeding sows and piglets

1980 ◽  
Vol 52 (1) ◽  
pp. 50-58
Author(s):  
Matti Näsi ◽  
Timo Alaviuhkola

In a factorial 2x2 experiment the use of a polyol mixture (sugar alcohols) in the diet of sows and piglets was investigated. The trial was performed with 26 sows divided into two groups. The polyol sows were fed 180 g polyol mixture daily, and for polyol group piglets the polyol supplementation in creep feed was 5 %. The average number of piglets per litter in the control group was 10.3 and in the polyol group 9.2. The addition of polyols to the diet of sows had only a small effect on the performance of piglets. The piglets receiving polyols in creep feed gained during 1—21 days post partum 21.1 % more than controls and during the rest of the lactation period 7 % more (P > 0.05). The incidence of diarrhoea in piglets of polyol groups was slightly higher compared with controls. The consumption of creep feed supplemented with polyols tended to be higher than consumption of control feed. Colostrum and milk samples were taken for analysis within 12 h after feeding and 7 and 21 days thereafter. The protein and lactose contents of colostrum and milk were slightly higher and the fat content lower from sows receiving polyols, but the differences were not significant. The mineral and amino acid composition was also analysed.

Author(s):  
F.I. Vasilevich ◽  
V.M. Bachinskaya ◽  
Yu.V. Petrova

Экспериментальные исследования кормовых добавок Абиотоник и Чиктоник проводили на базе вивария кафедры эпизоотологии и организации ветеринарного дела, а ветеринарносанитарную экспертизу продуктов убоя цыплятбройлеров проводили на кафедре паразитологии и ветеринарносанитарной экспертизы ФГБОУ ВО МГАВМиБ МВА имени К.И. Скрябина и ФГБНУ ФНЦ ВИЭВ РАН, аминокислотный состав мяса перепелов в Государственном бюджетном учреждении Краснодарского края Кропоткинская краевая ветеринарная лаборатория . Из цыплят в суточном возрасте кросса Кобб500 было сформировано три группы по 10 голов в каждой две опытные и контрольная опытным группам выпаивали кормовые добавки из расчета 1 мл/кг живой массы птицы до 50 суток выращивания, убой птицы проводили на 56 сутки. Тушки птицы после 24х часов созревания в холодильной камере при температуре 4 С подвергали исследованиям по общепринятым методикам: ГОСТ Р 519442002. Мясо птицы. Методы органолептических показателей, температуры и массы ГОСТ 314702012. Мясо птицы, субпродукты и полуфабрикаты из мяса птицы. Методы органолептических и физикохимических исследований аминокислотный состав мяса исследовали согласно М 0438 2009. Корма, комбикорма и сырье для их производства. Методика измерений массовой доли аминокислот методом капиллярного электрофореза с использованием системы капиллярного электрофореза Капель. Применение кормовых добавок в дозе 1 мл/кг живой массы способствовало увеличению живой массы птицы при применении Абиотоника на 54,23, а при применении Чиктоника на 37,70 по отношению к контролю. Во всех исследуемых пробах количество ЛЖК находится в пределах нормы и составило: в 1й опытной 1,390,03 мг КОН, во 2й опытной 1,420,04 мг КОН и в контрольной группе 1,810,06 мг КОН, что говорит о свежести и доброкачественности мяса. Значение рН мяса цыплятбройлеров находилось в трех группах в пределах нормы и не превышало 6,0. По результатам проведенных исследований аминокислотного состава красной и белой мышечной ткани цыплятбройлеров было установлено, что применение кормовой добавки Абиотоник способствовало увеличению незаменимых аминокислот на 12,14 и на 22,84 соответственно, а заменимых на 8,11 и на 22,51 по отношению к контрольной группеExperimental studies of feed additives Abiotonik and Chiktonik were conducted on the basis of the vivarium of the Department of Epizootology and Organization of Veterinary, and the veterinarysanitary examination of the products of slaughter broiler chickens was carried out at the Department of Parasitology and VeterinarySanitary Expertise of FSBEI HE MGAVMiB MBA named after KI Scriabin and the FSBI of the Federal Research Center of the VIEW RAS, the amino acid composition of quail meat in the State budget institution of the Krasnodar Territory Kropotkinskaya regional veterinary laboratory. Three groups of 10 animals each were formed from chickens at the daily age of the Cobb500 crosscountry, the experimental groups were fed feed additives at the rate of 1 ml / kg of live weight of poultry for up to 50 days of cultivation, and poultry were slaughtered for 56 days. Poultry carcasses after 24 hours of maturation in a refrigerating chamber at a temperature of 4 C were subjected to research according to generally accepted methods: GOST R 519442002. Poultry meat Methods of organoleptic characteristics, temperature and mass GOST 314702012 Poultry meat, offal and semifinished products from poultry meat. Methods of organoleptic and physicochemical studies) Amino acid composition of meat was carried out according to M 04382009. Feed, feed and raw materials for their production. Methods of measuring the mass fraction of amino acids by capillary electrophoresis using the Cappel capillary electrophoresis system. The use of feed additives in a dose of 1 ml / kg of live weight contributed to an increase in live weight of the bird when using Abiotonics by 54.23, and when using Chictonics by 37.70 relative to the control. In all studied samples, the number of VFAs is within the normal range and amounted to 1.39 0.03 mg KOH in 1 experimental group, 1.42 0.04 mg KOH in 2 experimental groups and 1.81 0.06 in the control group. mg KOH, which speaks of the freshness and goodness of meat. The pH of broiler chicken meat was in three groups within the normal range and did not exceed 6.0. According to the results of studies of the amino acid composition of red and white muscle tissue of broiler chickens, it was found that the use of the feed additive Abiotonik contributed to an increase in essential amino acids by 12.14 and by 22.84, and by replaceable ones by 8.11 and by 22.51 relative to the control group.Экспериментальные исследования кормовых добавок Абиотоник и Чиктоник проводили на базе вивария кафедры эпизоотологии и организации ветеринарного дела, а ветеринарносанитарную экспертизу продуктов убоя цыплятбройлеров проводили на кафедре паразитологии и ветеринарносанитарной экспертизы ФГБОУ ВО МГАВМиБ МВА имени К.И. Скрябина и ФГБНУ ФНЦ ВИЭВ РАН, аминокислотный состав мяса перепелов в Государственном бюджетном учреждении Краснодарского края Кропоткинская краевая ветеринарная лаборатория . Из цыплят в суточном возрасте кросса Кобб500 было сформировано три группы по 10 голов в каждой две опытные и контрольная опытным группам выпаивали кормовые добавки из расчета 1 мл/кг живой массы птицы до 50 суток выращивания, убой птицы проводили на 56 сутки. Тушки птицы после 24х часов созревания в холодильной камере при температуре 4 С подвергали исследованиям по общепринятым методикам: ГОСТ Р 519442002. Мясо птицы. Методы органолептических показателей, температуры и массы ГОСТ 314702012. Мясо птицы, субпродукты и полуфабрикаты из мяса птицы. Методы органолептических и физикохимических исследований аминокислотный состав мяса исследовали согласно М 0438 2009. Корма, комбикорма и сырье для их производства. Методика измерений массовой доли аминокислот методом капиллярного электрофореза с использованием системы капиллярного электрофореза Капель. Применение кормовых добавок в дозе 1 мл/кг живой массы способствовало увеличению живой массы птицы при применении Абиотоника на 54,23, а при применении Чиктоника на 37,70 по отношению к контролю. Во всех исследуемых пробах количество ЛЖК находится в пределах нормы и составило: в 1й опытной 1,390,03 мг КОН, во 2й опытной 1,420,04 мг КОН и в контрольной группе 1,810,06 мг КОН, что говорит о свежести и доброкачественности мяса. Значение рН мяса цыплятбройлеров находилось в трех группах в пределах нормы и не превышало 6,0. По результатам проведенных исследований аминокислотного состава красной и белой мышечной ткани цыплятбройлеров было установлено, что применение кормовой добавки Абиотоник способствовало увеличению незаменимых


2011 ◽  
Vol 105 (12) ◽  
pp. 1764-1771 ◽  
Author(s):  
Julia Gaye-Siessegger ◽  
James S. O. McCullagh ◽  
Ulfert Focken

The aim of the present study was to test whether the dietary non-essential/conditionally essential amino acid composition has an effect on growth and protein utilisation and on δ13C of individual amino acids in rainbow trout (Oncorhynchus mykiss). Trout were reared on six purified diets containing only synthetic amino acids in place of protein. Diet 1 mimicked the amino acid composition of fishmeal, in diet 2, cysteine (Cys), glycine (Gly), proline (Pro) and tyrosine (Tyr) were isonitrogenously replaced by their precursor amino acids serine (Ser), glutamic acid (Glu) and phenylalanine (Phe), and in diet 3, alanine (Ala), asparagine and aspartate, Cys, Gly, Pro, Ser and Tyr were isonitrogenously replaced by Glu. Diets 4, 5 and 6 resembled diets 1, 2 and 3 except that Glu contained 0·1 % 13C-enriched Glu. A control group was reared on a fishmeal-based diet. A total of forty-two trout (4·7 (sd 0·57) g) were fed one of the diets at a level of 3·5 % body mass for 10 weeks in a flow-through system. Dietary non-essential amino acid composition significantly influenced protein gain (P < 0·025) and δ13C of Ala, arginine (Arg), Gly, histidine (His), Phe and Tyr. Non-enriched Glu was predominantly found in trout fed 13C-enriched Glu, which is consistent with the fact that Glu has been shown to be used extensively in the gut as an energy source but is less consistent with the enrichment of Pro in fish fed diet 6 compared with fish fed diet 3. Further research is required to better understand the mechanisms that lead to the alteration of amino acid δ13C between diet and body tissues.


Author(s):  
Eva Ivanišová ◽  
Barbara Mickowska ◽  
Peter Socha ◽  
Ivana Režová ◽  
Attila Kántor ◽  
...  

Abstract The aim of this work was to characterise the biological and sensory profile of biscuits enriched with green (1 and 3%) and black tea (1 and 3%) powders. Biscuits without the addition of tea were used as a control. Phenolic concentration, flavonoid concentration, and antioxidant activity were determined spectrophotometrically. Amino acid composition was determined using automatic amino acid analyser AAA 400 and crude fibre content using an Ancom analyser. Sensory profiles were evaluated by comparison of enriched and control biscuit samples. The enriched biscuits showed higher phenolic and flavonoid concentration and antioxidant activity estimated by DPPH and phospholybdenum method in comparison with levels in the control group. The best results for antioxidant activity estimated by DPPH and phosphomolybdenum methods were achieved in biscuits enriched with black tea powder (3%): 2.25 and of 32.64 mg TEAC·g−1, respectively. Total phenolic concentration was 1.16 mg GAE·g−1, and total flavonoid concentration was 0.13 mg QE·g−1. These biscuits had higher concentration of crude fibre in comparison with the control group and the highest concentration (0.64%) was found in biscuits with addition of 3% green tea powder. The amino acid composition in samples, including in the control sample was balanced, with slightly higher concentration of threonine, serine, and methionine in enriched samples, but this parameter was not statistically significant. Biscuits enriched with green and black tea had higher sensory scores for taste, smell and aftertaste.


10.5219/1601 ◽  
2021 ◽  
Vol 15 ◽  
pp. 497-505
Author(s):  
Vadym Kondratiuk ◽  
Volodymyr Otchenashko

The article studies the effect of using complete compound feeds with different energy levels on the morphological composition of the body and the amino acid composition of trout meat proteins. The experiment aimed to establish the influence of different levels of energy nutrition of commercial rainbow trout on the morphological composition of their body and the amino acid composition of meat proteins. For this purpose, five experimental groups were formed using the analog method. The study lasted 210 days and was divided into two periods: comparative (10 days) and main (200 days). During the comparative period, the study fish consumed compound feed of the control group. During the main period, the energy level in experimental compound feeds for different experimental trout groups ranged from 16 to 20 mJ per 1 kg. It was found that with an increase in the mass of two-year-old trout, the mass of muscle tissue and the yield of edible parts probably increases. Feeding fish with an increased amount of metabolic energy (20 mJ.kg-1) resulted in a significant change in the weight of internal organs, including the heart, liver, and kidneys. Increasing the metabolic energy in the compound feeds of fish from 18 mJ.kg-1 to 19 – 20 mJ.kg-1 leads to a significant increase in bowel mass by 13.3 – 5.0%. An increase in the level of metabolic energy in rainbow trout diets from 18 mJ.kg-1 to 19 – 20 mJ.kg-1 contributed to a likely increase in the methionine content in meat. A similar pattern was observed for the tryptophan content. It was found that the content of most essential amino acids in the protein of rainbow trout meat exceeds the corresponding values in the "ideal" protein, except for the content of isoleucine and leucine, which refers them to limiting amino acids.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mukund Gauthankar ◽  
Rakhee Khandeparker ◽  
Mamatha S. Shivaramu ◽  
Komal Salkar ◽  
Rayadurga Anantha Sreepada ◽  
...  

AbstractFish silage is a brown liquefied product achieved by the action of enzymes when finely grounded whole/parts of either single or mixed fish types are subjected to acidification. This study made a comparative assessment of biochemical and nutritive properties, especially the amino acid composition in supernatant phase of formic acid silages prepared from two fish types, Indian mackerel (Rastrelliger kanagurta) and false travely (Lactarius lactarius) representing fat fish (FF, fat content > 5%) and lean fish (LF, fat content < 5%), respectively during 35 days of fermentation (DoF). Significantly higher content of total amino acid (TAA) and free amino acids (FAA) were recorded in FFS (TAA, 41.2 ± 0.03 mg/g; FAA, 31.3 ± 0.003 mg/g) compared to LFS (TAA, 35.8 ± 0.07 mg/g; FAA, 18.26 ± 0.003 mg/g; FAA, 31.3 ± 0.003 mg/g) (p < 0.05). At the end of 35 DoF, the concentrations of amino acids such as asparagine, histidine, isoleucine, valine, cysteine, serine, lysine and arginine were significantly higher in FFS as compared to LFS. The relative amino acid composition of FFS and LFS varied in accordance with DoF and the relationship was found to be highly significant (ANOVA, p < 0.00001). High concentrations of l-amino acids such as leucine, glutamic acid and arginine were recorded in both FFS and LFS. In conclusion, the analysis suggested that a fermentation period of 25–30 days showed a significant effect on the composition of amino acids in both types of ensilage compared to other fermentation periods (p < 0.05). Considering the role of amino acids in enhancing the plant growth and proliferation, the findings of the present study are quite useful.


Author(s):  
D. O. Oshibanjo ◽  
O. O. Olusola ◽  
O. A. Ogunwole

Aims: The effect of cooking method and temperature on amino acid composition of breakfast sausage (BS) was undertaken in this study. Methodology: Three batches of prepared BS from beef, were randomly allotted to three cooking methods (CM): (boiling, grilling and frying) each at cooking temperatures (CT) of 80, 90 and 100°C to attain internal temperature of 72°C in a completely randomized design. Samples from each treatment were oven-dried and assayed for amino acid and proximate composition using standard procedures. Data obtained was analysed using descriptive statistic and ANOVA at α0.05. Results: Results showed that grilled sausage at 80°C had highest total amino acid profile (3.2%). Grilled sausage at 80°C had highest crude protein (25.58%). Grilled BS at 80°C recorded least fat content (15.99%). Grilled sausage at 80°C had the higher ash (6.66%) and least (1.40%) in boiled sausage at 90°C. Conclusion: Therefore, breakfast sausage could be best grilled at 80°C due to maintain high amino acid profile, crude protein, ash and lower fat content.


1968 ◽  
Vol 106 (3) ◽  
pp. 749-757 ◽  
Author(s):  
J J Harding ◽  
J. M. Wesley

Collagens and gelatins were isolated from human post-menopausal uterus, puerperal (post-partum) uterus, rheumatoid-arthritis-nodule and ox tendon. Different means of purifying collagen were studied and a method was devised that enables highly purified collagen to be obtained, even from the uterus. This method involves the use of a number of aqueous and organic extractants as well as digestion with elastase to eliminate elastin. The purity of the collagen preparations was assessed and they were used to study the amino acid composition of collagen. The amino acid compositions of all the collagens studied were similar to those of human bone and tendon collagen, but certain small differences were noted and are discussed. The soluble collagen extracted from some of the tissues was also studied.


2020 ◽  
Author(s):  
Giovanni S. Molinari ◽  
Michal Wojno ◽  
Vance J. McCracken ◽  
Karolina Kwasek

Soybean meal (SBM) is the most common source of protein used to replace fishmeal (FM) in aquaculture diets. SBM inclusion in diets has been found to negatively affect growth and induce intestinal inflammation in fish. The objective of this study was to determine the effect of health-promoting dipeptide (carnosine, anserine, alanyl-glutamine) supplementation into SBM-based feeds on growth performance, intestinal health, and muscle free amino acid composition, an indicator of dietary amino acid availability, in a zebrafish model. There were 5 treatment groups in this study. The first group ( (+) Control ) received a FM-based diet. The second group ( (-) Control ) received SBM-based diet. The last three groups ( Ala-Glu, Car, and Ans) were fed SBM-based diets, supplemented with alanyl-glutamine, carnosine, and anserine respectively. All groups received their respective diets during 33-59 dph. The Ala-Glu and Car groups experienced a significantly higher weight gain than the (-) Control group, weighing 35.38% and 33.96% more, respectively at the conclusion of the study. There were no significant differences among gene expression between the groups, but Ala-Glu had the highest expression of both nutrient absorption genes measured, PepT1 and fabp2 . Ala-Glu had significantly longer intestinal villi, and a significantly higher villus length-to-width ratio than the (-) Control group. Among the free amino acid composition, the Car group had a significantly higher post-prandial concentration of lysine, compared to the (-) Control group. The increase in villi surface area and expression of nutrient absorption genes represent an improvement in intestinal absorptive capacity in the Ala-Glu group. The increase in lysine concentration may signify an increase in the retention of protein in the fish in the Car group. The results from this study provide support for the use of alanyl-glutamine and carnosine supplementation as a means of improving growth performance of zebrafish fed with a 100% SBM-based diet.


2020 ◽  
Vol 27 ◽  
pp. 00134
Author(s):  
L. A. Shadieva ◽  
E. M. Romanova ◽  
V. N. Lyubomirova ◽  
V. V. Romanov ◽  
T. M. Shlenkina

The article outlines the results of the research into the influence of feed composition on the amino acid value of African catfish meat. It has been shown that quality characteristics of fish meat depend on protein and fat content. It has been proved that high-protein feed ensures increase in the content of all amino acids in African catfish meat. Nevertheless, protein and fat content in the muscles of the studied fish is more than 2 times higher than the same indicator in the fish on low-protein and low-fat diet. Meat of the African catfish is rich in two amino acids – leucine and lysine. Two amino acids, tryptophan and methionine, are limitative at a high protein diet. At a lower protein diet, isoleucine amino acid is also added. The amino acid composition of African catfish meat is highest at high-protein feeds. The amino acid index of African catfish muscles at high-protein feeds is 0.48, significantly exceeding the index of fish bred on feeds with a reduced protein content. The conducted studies have shown that the use of high-protein feeds in catfish breeding stimulates protein metabolism, enriching the amino acid composition of muscle tissue and increasing the nutritional value of fish as a food product. The research has been funded by the Russian Foundation for Fundamental Research, project No. 18-016-00127.


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