scholarly journals Рredominant microflora of natural and wastewaters of Lviv region

2020 ◽  
Vol 3 (2) ◽  
pp. 121-126
Author(s):  
I. Z. Koval ◽  
Keyword(s):  
Industrial Wastewater ◽  
Natural Waters ◽  
Petri Dish ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Wort Agar ◽  
Blue Green Algae ◽  
Microbiological Methods ◽  
Meat Peptone Agar ◽  
Saccharomyces Yeast

Quantitative and qualitative composition of microorganisms have been determined by the microbiological methods of the analysis of natural waters and industrial wastewater. It is shown that the dominant microflora of the studied waters are Diplococcus, Sarcina, Bacillus, Pseudomonas bacteria types, blue-green algae of Oscillatoria types, as well as Saccharomyces yeast types. Morphological, physiological and cultural characteristics of colonies grown on nutrient media were studied to identify microorganisms. The growth pattern of colonies of microorganisms on meat-peptone agar (for bacteria) and wort-agar (for yeast) in a Petri dish is shown.

scholarly journals FLAVONOIDS OF IRIS SIBIRICA L. GROWN IN VITRO CULTURE

2021 ◽  
pp. 301-308
Author(s):  
Olesya Nikolayevna Mazko ◽  
Lyudmila Ivanovna Tikhomirova ◽  
Lyudmila Vladimirovna Shcherbakova ◽  
Natal'ya Grigor'yevna Bazarnova ◽  
Dmitriy Alekseyevich Karpitsky
Keyword(s):  
In Vitro Culture ◽  
High Performance ◽  
Raw Materials ◽  
Quantitative Composition ◽  
Qualitative Composition ◽  
Nutrient Media ◽  
Qualitative And Quantitative ◽  
Clonal Micropropagation ◽  
Intact Plants

The aim of this study was to evaluate the effect of 6-benzylaminopurine (BAP) separately and in interaction with auxins on the change in the qualitative and quantitative composition of flavonoids in the raw materials of regenerating plants Iris sibirica L. Cambridge grade in comparison with aeroponic and intact raw materials using the method of high-performance liquid chromatography. Raw materials of I. sibirica Cambridge variety obtained in vitro culture had a richer qualitative composition of flavonoids than intact plants. The dependence of the accumulation of flavonoids on the concentration of 6-benzylaminopurine in nutrient media was noted. The presence of 13 compounds was observed in extracts of 70% ethyl alcohol from regenerating plants grown at the lowest concentration of BAP (1.0 µM) within the experiment. In quantitative terms, the flavonoid apigenin was maximally determined on a medium with BAP 1 µM, and kaempferol - on media with BAP 5.0 µM, supplemented with auxins. For a medium with 7.5 µM BAP, the lowest variety of compounds was observed (9) and the lowest kaempferol content. Auxins influenced the synthesis of flavonoids. The amount of flavonoids in all variants of the experiment increased by an average of 13% in the presence of auxins. The stages of the technological process of obtaining raw materials I. sibirica Cambridge variety on the basis of clonal micropropagation and cultivation in aeroponics conditions allowed to obtain raw materials that do not contain heavy and toxic metals, are not infected with pathogens and pests. With 1 m2 of useful area of aeroponics for 1 year, it is possible to collect 5 times more raw materials than with field cultivation. According to the qualitative composition of phenolic compounds, aeroponic raw materials are identical to intact plants.

scholarly journals Treatment of natural surface waters using new composite flocculants-coagulants

2020 ◽  
Vol 4 (5) ◽  
pp. 211-217
Author(s):  
Kudryavtsev P
Keyword(s):  
Water Purification ◽  
Industrial Wastewater ◽  
Natural Waters ◽  
Drinking Water Treatment ◽  
Titration Method ◽  
Aluminum Salts ◽  
Iron Salts ◽  
Isoelectric Points ◽  
Ancient Times ◽  
Hydrolysis Of

Coagulation is an essential process in the treatment of water and industrial wastewater. In the field of drinking water treatment since ancient times, water purification using coagulants using various substances has been practiced. Coagulation is the most common method of purification of natural and wastewaters from the bulk of colloidal, finely dispersed, and partially dissolved contaminants. The characteristics of the new composite flocculants-coagulants ASFC and ISFC developed by the authors are presented in comparison with conventional aluminum and iron salts. The titration method was used to study the behavior of coagulant flocculants at different pH. The titration method was used to study the behavior of coagulant flocculants at different pH. The ζ-potentials and isoelectric points for aluminum hydroxide obtained by hydrolysis of a coagulant are determined. The nature of the interaction of active silicic acid and the products of hydrolysis of aluminum salts as components of composite flocculants-coagulants is shown. The characteristics of natural waters are given, on which the efficiency of using the obtained flocculants-coagulants is investigated. A comparison of the coagulating ability of coagulants in water purification with high color from the Orsha River and with medium color from the Volga and Tvertsa rivers is presented.

scholarly journals FORMATION OF THE EUBACTERIAL COMPLEX IN THE RHYOSPHERE OF SUGAR BEET (Beta vulgaris) UNDER DIFFERENT FERTILIZATION SYSTEMS

2021 ◽  
Vol 14 (1) ◽  
pp. 81-87
Author(s):  
S. O. Hudz ◽  
◽  
◽  
Keyword(s):  
Species Diversity ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Biological System ◽  
Molecular Methods ◽  
Taxonomic Structure ◽  
Qualitative Composition ◽  
Soil Microbiota ◽  
Microbiological Methods ◽  
System Of Fertilizers

The aim of the work was to determine the quantitative, qualitative composition and taxonomic structure of the eubacterial complex in the rhizosphere of sugar beet under different fertilizer systems. Microbiological methods were used to determine the content of microorganisms in the rhizosphere of sugar beet. Molecular methods were used to determine taxonomic structure as well as metagenome of the eubacterial complex of microorganisms. In the agrocenosis of sugar beet under different fertilizer systems the representatives of such families were prevailed as Alcaligenaceae, Pseudomonadaceae, Nitrososphaeraceae, Gaiellaceae, Micrococcaceae, Solirubrobacteraceae, Streptomycetaceae, Intrasporangiaceae, Solimonadaceae, Syntrophobacteraceae, Xanthomonadaceae, Enterobacteriaceae, Nocardioidaceae, Hyphomicrobiaceae, Comamonadaceae. It was found that under the biological system of fertilizers the species diversity of soil microbiota increased due to phyla: Alcaligenaceae, Gaiellaceae, Solirubrobacteraceae, Streptomycetaceae, Solimonadaceae, Syntrophobacteraceae, Xanthomonadaceae, Enterobacteriaceae, Nocardioidaceae, Hyphomicrobiaceae, Hyphomicrobiaceae. It was detected that the basis of the eubacterial complex of sugar beet included representatives of phyla Proteobacteria, Actinobacteria, Gemmatimonadetes, Chloroflexi, Acidobacteria, Firmicutes, Planctomycetes, Verrucomicrobia, Bacteroidetes, and the absolute dominants were Proteobacteria – 76.9%, Actinobacteria – 13,4 %.

scholarly journals Natural Zeolites of Eastern Transbaikalia in Technologies for Mining Enterprises Wastewater Treatment

2020 ◽  
Vol 76 (3) ◽  
pp. 62-70
Author(s):  
Konstantin Razmakhnin ◽  
Vitaliy Vitalievich Milyutin ◽  
Alisa Nikolaevna Khatkova ◽  
Nataliya Anatolevna Nekrasova
Keyword(s):  
Exchange Rate ◽  
Industrial Wastewater ◽  
Natural Waters ◽  
Natural Zeolites ◽  
Adsorption Interaction ◽  
Cesium Radionuclides ◽  
Eastern Transbaikalia ◽  
Synthetic Zeolites ◽  
The Impact ◽  
Sorption Characteristics

The possibilities of conditioning acidified wastewater from mining enterprises by natural zeolites of deposits in East Transbaikalia were studied. Sorption characteristics of various natural and synthetic zeolites in relation to strontium and cesium radionuclides were determined to evaluate the possibility of their application for the purification of radioactively contaminated technogenic and natural waters. The ability of natural zeolites to selectively adsorb the heavy metal ions was investigated. An experimental study was made on the possibility of neutralizing acidic media with zeolites. The kinetic dependencies were obtained to evaluate the ion exchange rate on the natural zeolites. The impact of exchanging cations and the defining mineral’s structure on the exchange rate was studied. The instantaneous adsorption interaction rate values were determined. The possibility of 90Sr and Ca2+ ions sorption from the simulated solutions of industrial wastewater was investigated.

scholarly journals Designing a Nutrient Medium for the Accumulation of Microbial Mass of Listeria

2020 ◽  
Vol 5 (4) ◽  
pp. 60-66
Author(s):  
N. M. Khaptanova ◽  
S. V. Lukyanova ◽  
V. I. Kuznetsov ◽  
N. G. Gefan ◽  
N. M. Аndreevskaya ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Specific Activity ◽  
Germination Rate ◽  
Biochemical Properties ◽  
Quantitative Composition ◽  
Nutrient Media ◽  
Microbiological Methods ◽  
Microbial Mass

Background. To obtain reliable results of laboratory studies on the identification of Listeria, the presence of certified diagnostic agglutinating Listeria sera is required. An important step in the manufacturing process of such medical devices for in vitro diagnostics requires effective nutrient media for the accumulation of listeriosis microbe. Aim of the research. To develop an effective nutrient medium for the accumulation of bacterial mass of Listeria. Materials and methods. The object of the study was an experimental culture medium for Listeria cultivation. As a control, we used nutrient agar for the cultivation of microorganisms (fish meal hydrolysate, FMH-agar) and meat-peptone agar with 1 % glucose (MPA with 1 % glucose). The specific activity of nutrient media during cultivation of the test strain Listeria monocytogenes 766 was evaluated using a complex of microbiological methods. Results. The optimal base of the nutrient medium for Listeria cultivation has been selected: pancreatic hydrolysate of river magpie fish (Rutilus rutilus lacustris) and hydrolysate of meat water production waste. The qualitative and quantitative composition of the nutrient medium has been developed, its physical, chemical and biological properties have been studied. It was found that after 24 hours of incubation at 37 °C, the nutrient medium provided the growth of typical Listeria colonies. The germination rate was 85 %, which is higher compared to the growth of the culture on MPA with 1 % glucose and GRM agar by an average of 21 % (p < 0,05). Conclusion. The experimental culture medium for Listeria cultivation provided growth of colonies of the test strain L. monocytogenes 766 with the preservation of characteristic cultural, morphological and biochemical properties, and, in terms of germination and growth rate, exceeded the control media. The developed nutrient medium provides effective growth of Listeria and can be used as a medium for the accumulation of microbial mass.

scholarly journals Recommendations for the Aluminum and Iron-Containing Composite Flocculants-Coagulants Use for the Purification of Natural River Waters and Industrial Wastewater

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Kudryavtsev P
Keyword(s):  
Industrial Wastewater ◽  
Natural Waters ◽  
Magnetic Treatment ◽  
Wastewater Purification ◽  
River Waters ◽  
Industrial Wastewaters ◽  
Iron Salts

Coagulation is the most widespread method of natural and wastewater purification from the bulk of colloidal, finely dispersed, and partially dissolved contaminants. The characteristics of the new composite flocculants-coagulants ASFC and ISFC developed by the authors compared to traditional aluminum and iron salts are given. The characteristics of natural waters, which were used to study the efficiency of using the obtained flocculants-coagulants, are given. To compare the effect of the developed materials with known products, we used such a parameter as their coagulation ability to purify high-color water from the Orsha river and medium-color water from the Volga and Tvertsa rivers in Russia. An attempt is made to assess the effect of magnetic treatment of the developed composite flocculants-coagulants. Based on the conducted research and production tests, recommendations were developed to use aluminum and iron-containing composite flocculants-coagulants to purify natural river waters and industrial wastewaters.

INDUSTRIAL WASTEWATER PURIFICATION FROM OIL AND HEAVY METALS

2014 ◽  
Vol 4 (3) ◽  
pp. 60-64
Author(s):  
Vladimir Dmitrievich NAZAROV ◽  
Konstantin Konstantinovich BARYKIN ◽  
Sergey Vladimirovich FURSOV
Keyword(s):  
Heavy Metals ◽  
Surface Waters ◽  
Industrial Wastewater ◽  
Natural Waters ◽  
Water Protection ◽  
Wastewater Purification ◽  
Republic Of Bashkortostan ◽  
Water Cleaning ◽  
The Republic ◽  
Quantitative Indicators

The main industries of the Republic of Bashkortostan discharging pollutants to surface waters are presented. The quantitative indicators of natural waters volumes abstracted by enterprises of fuel, chemical and petrochemical industries are viewed as well as mass of pollutants discharged with sewage. The basic problems of the industry of water protection are presented. Device for water cleaning from oil and heavy metals based on galvanochemical method of water treatment is proposed and described. The results of industrial water cleaning from a number of impurities by galvano coagulation are viewed.

Cyanobacterial Toxins in Water

1989 ◽  
Vol 21 (3) ◽  
pp. 1-13 ◽  
Author(s):  
Geoffrey A. Codd ◽  
Steven G. Bell ◽  
William P. Brooks
Keyword(s):  
Drinking Water ◽  
Natural Waters ◽  
Biological Significance ◽  
Industrial Effluents ◽  
Toxin Production ◽  
Immunological Methods ◽  
Mouse Bioassay ◽  
Cyanobacterial Toxins ◽  
Blue Green Algae ◽  
Cyanobacterial Toxin

Cyanobacteria (blue-green algae) commonly occur in fresh- and brackish waters and may produce massive annual growths as a consequence of nutrient enrichment from natural waters, agricultural fertilizer run-off, or from domestic/industrial effluents. The cyanobacterial species which dominate these growths typically belong to the genera which produce toxins. Cyanobacterial toxins cause fatal poisonings of agricultural livestock, wild animals, birds and fish on a world-wide basis. The involvement of the toxins in human health problems has also been inferred in several countries and their presence in drinking water sources is of interest to the drinking water industry. The occurrence and properties of cyanobacterial toxins are discussed here. New methods are being developed for the purification of the toxins and for their recovery and quantification from waters. These include the use of chemical, cytotoxicity and immunological methods to complement the mouse bioassay which has hitherto been used in cyanobacterial toxin studies with laboratory cultures and water samples. Information on the regulation of cyanobacterial toxin production and on the possible biological significance of the toxins in aquatic environments is also presented. A greater awareness of cyanobacterial toxins in waters destined for human use is required.

scholarly journals Comparative evaluation of hydrolysates as a basis for the construction of a nutrient medium for the cultivation of Listeria monocytogenes

2021 ◽  
Author(s):  
N. M. Khaptanova ◽  
А. S. Ostyak ◽  
S. V. Lukyanova ◽  
V. I. Kuznetsov ◽  
N. M. Аndreevskaya ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Enzymatic Hydrolysis ◽  
Nutrient Medium ◽  
Comparative Evaluation ◽  
Raw Materials ◽  
Amino Nitrogen ◽  
Test Strain ◽  
Nutrient Media ◽  
Microbiological Methods ◽  
The Common

The objective is to perform a comparative evaluation of the pancreatic hydrolysates prepared from fish and squid to determine the optimal culture medium for Listeria monocytogenes.Materials and methods. The following raw materials were used in the study: Pacific Herring (Clupea pallasii), Alaska Pollock (Gadus chalcogrammus), Common Roach (Rutilus rutilus lacustris), European Squid (Loligo vulgaris). The raw materials were subjected to enzymatic hydrolysis using the pancreas (according to Hottinger). A study of the physicochemical properties of pancreatic hydrolysates (content of free amino nitrogen (FAN), acidity of fish hydrolysates, the amino acid composition) was carried out.. The specific activity of nutrient media during the cultivation of the test strain L. monocytogenes 766 was assessed by a complex of microbiological methods.Results and discussion. The highest content of FAN at the end of enzymatic hydrolysis was observed in the pancreatic hydrolysate of the common roach (6%), the acidity of the hydrolysate remained stable from 6th to 13th day of the hydrolysis process (pH 7.2). Pancreatic hydrolysate of the common roach contained a number of amino acids that are most essential for the growth of Listeria. An assessment of the biological properties of nutrient media prepared on the basis of the obtained hydrolysates demonstrated that the best results in terms of sensitivity and germination of L. monocytogenes 766 showed a nutrient medium based on the pancreatic hydrolysate of the common roach. During the cultivation of L. monocytogenes 766 the test strain retained its morphological and cultural properties and did not show signs of dissociation.Conclusion. The research results have shown that the pancreatic hydrolysate of the common roach is a promising protein basis for the construction of an experimental environment for listeria. 

scholarly journals Magnetically controlled microorganisms for the biological industrial wastewater treatment

2020 ◽  
Vol 10 (2) ◽  
pp. 185-200
Author(s):  
Irina V. Vladimtseva ◽  
Irina V. Mogilevskaya ◽  
Olga V. Kolotova ◽  
Valery E. Drevin
Keyword(s):  
Electromagnetic Field ◽  
Wastewater Treatment ◽  
Industrial Wastewater ◽  
Bacterial Cells ◽  
Bacterial Strains ◽  
Nutrient Media ◽  
Industrial Wastewater Treatment ◽  
Magnetic Carriers ◽  
Effective Technology ◽  
Biomass Increase

Microorganisms immobilized on magnetic carriers are used in biotechnology to increase efficiency and simplify work with microbial cells. The aim of this study was to obtain magnetically controlled immobilized forms of the environmentally significant microorganisms and to study the possibility of their cultivation with artificial nutrient media. Two bacterial strains, identified as the genus Bacillus, were used as the research object: B. subtilis ВГТУ05 and B. species ВГТУ06, promising for biological industrial wastewater treatment. The work carried out the immobilization of strains in magnetic alginate carriers. Established the bacterial cells’ immobilization did not affect their vitality. The optimal parameters of the electromagnetic field strength for growing strains in liquid nutrient media were selected. Shown the bacterial destructive strains immobilized into magnetic carriers gave the biomass increase of 24.3–25.0% during the cultivation in the electromagnetic field. The results could be used to develop the effective technology for local biological industrial wastewater treatment.

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