Decentralized Retunability under Structural Perturbations

1982 ◽  
Author(s):  
U. Ozguner ◽  
E.J. Davison
Keyword(s):  
Structural Perturbations

scholarly journals Structural Changes in the Acceptor Site of Photosystem II upon Ca2+/Sr2+ Exchange in the Mn4CaO5 Cluster Site and the Possible Long-Range Interactions

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 371
Author(s):  
Koua
Keyword(s):  
Crystal Structure ◽  
Photosystem Ii ◽  
Long Range ◽  
Electron Acceptor ◽  
Structural Changes ◽  
Acceptor Site ◽  
Oxygen Evolving Complex ◽  
Long Range Interactions ◽  
Structural Perturbations ◽  
Oxygen Evolving

The Mn4CaO5 cluster site in the oxygen-evolving complex (OEC) of photosystem II (PSII) undergoes structural perturbations, such as those induced by Ca2+/Sr2+ exchanges or Ca/Mn removal. These changes have been known to induce long-range positive shifts (between +30 and +150 mV) in the redox potential of the primary quinone electron acceptor plastoquinone A (QA), which is located 40 Å from the OEC. To further investigate these effects, we reanalyzed the crystal structure of Sr-PSII resolved at 2.1 Å and compared it with the native Ca-PSII resolved at 1.9 Å. Here, we focus on the acceptor site and report the possible long-range interactions between the donor, Mn4Ca(Sr)O5 cluster, and acceptor sites.

scholarly journals Monomerization of far-red fluorescent proteins

2018 ◽  
Vol 115 (48) ◽  
pp. E11294-E11301 ◽  
Author(s):  
Timothy M. Wannier ◽  
Sarah K. Gillespie ◽  
Nicholas Hutchins ◽  
R. Scott McIsaac ◽  
Sheng-Yi Wu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Biological Markers ◽  
Fluorescent Proteins ◽  
Biological Tissues ◽  
Biological Applications ◽  
Emission Maximum ◽  
Naturally Occurring ◽  
Red Fluorescent Proteins ◽  
Animal Imaging ◽  
Structural Perturbations

Anthozoa-class red fluorescent proteins (RFPs) are frequently used as biological markers, with far-red (λem ∼ 600–700 nm) emitting variants sought for whole-animal imaging because biological tissues are more permeable to light in this range. A barrier to the use of naturally occurring RFP variants as molecular markers is that all are tetrameric, which is not ideal for cell biological applications. Efforts to engineer monomeric RFPs have typically produced dimmer and blue-shifted variants because the chromophore is sensitive to small structural perturbations. In fact, despite much effort, only four native RFPs have been successfully monomerized, leaving the majority of RFP biodiversity untapped in biomarker development. Here we report the generation of monomeric variants of HcRed and mCardinal, both far-red dimers, and describe a comprehensive methodology for the monomerization of red-shifted oligomeric RFPs. Among the resultant variants is mKelly1 (emission maximum, λem = 656 nm), which, along with the recently reported mGarnet2 [Matela G, et al. (2017) Chem Commun (Camb) 53:979–982], forms a class of bright, monomeric, far-red FPs.

Structural perturbations within Ge nanocrystals in silica

2004 ◽  
Vol 84 (2) ◽  
pp. 278-280 ◽  
Author(s):  
A. Cheung ◽  
G. de M. Azevedo ◽  
C. J. Glover ◽  
D. J. Llewellyn ◽  
R. G. Elliman ◽  
...  
Keyword(s):  
Structural Perturbations ◽  
Ge Nanocrystals
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