scholarly journals Phenotypic and Molecular Characterization of Pathogenic Bacteria Isolated from Commercialised Herbal Products in Kenya

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Meshack O Onyamb
Keyword(s):  
Molecular Characterization ◽  
Pathogenic Bacteria ◽  
Herbal Products

Identification and molecular characterization of pathogenic bacteria in foods confiscated from non-EU flights passengers at one Spanish airport

2015 ◽  
Vol 209 ◽  
pp. 20-25 ◽  
Author(s):  
David Rodríguez-Lázaro ◽  
Jaime Ariza-Miguel ◽  
Marta Diez–Valcarce ◽  
Beatrix Stessl ◽  
Janine Beutlich ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Pathogenic Bacteria

Biochemical and molecular characterization of proteolytic bacterial strains isolated from Jazan region, KSA with the application as an antibacterial agent

2020 ◽  
Vol 3 ◽  
pp. 1
Author(s):  
Bander Mohammed Al-Thobaiti ◽  
Emad Abada ◽  
Khaled El-Gayar
Keyword(s):  
Enzyme Activity ◽  
Molecular Characterization ◽  
Bacterial Growth ◽  
Antibacterial Agent ◽  
Pathogenic Bacteria ◽  
Crude Enzyme ◽  
Genetic Identification ◽  
Rrna Gene ◽  
Bacterial Strains

Objectives: Biochemical and molecular characterization of proteolytic bacterial strains isolated from Jazan region, KSA with the application as an antibacterial agent. Materials and Methods: Three samples were collected from extreme environment, Jazan, KSA. Skim milk nutrient agar medium was used for protease screening for several colonies by streaking method at 37°C. API biochemical kit was used to characterize the three isolates using some selective media. The genetic identification was done using 16S rRNA gene sequencing. The sensitivity of the tested strains;Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae against the extracellular crude protease enzyme produced from the three isolated bacteria and different antibiotics was done. Results: The isolates were identified as Bacillus subtilis, Bacillus licheniformis, and Bacillus cereus. B. cereus and B. licheniformis recorded high sensitivity (71%) against most antibiotics, in addition, B. subtilis showed resistance to Aztreonam only. It was found a considerable increase in the level of both of protease activity (units/ml) and bacterial growth (colony-forming units/ml) of the cultures that were directed by the B. subtilis and B. licheniformis up to 37°C then decreased at 45°C. On the contrary, the growth of B. cereus and its activity gradually increased up to 45°C. The enzyme activity and bacterial growth of B. subtilis and B. cereus strains were increased at alkaline medium. However, B. licheniformis gave the highest growth and activity at neutral pH. In addition, it was found that the enzyme activity and bacterial growth of B. subtilis were reached to the maximum at 5% NaCl. However, the maximum bacterial growth and enzyme activity for B. licheniformis and B. cereus was at 2% NaCl. It was found high effect on inhibiting the growth of pathogenic bacteria using 5 μl of crude enzyme with specific enzyme activity 73, 76, and 92 (units/ml)/(mg protein/ml) for B. subtilis, B. licheniformis, and B. cereus, respectively. All pathogenic bacteria were totally inhibited with 10 μl of crude enzyme. Conclusion: The potential Bacillus proteases can promote new industry as antimicrobial agents.

scholarly journals Molecular characterization of corneal ulcers causing Staphylococcus aureus

2017 ◽  
Vol 5 (4) ◽  
pp. 1419
Author(s):  
Deepika N. Jain ◽  
Vilas A. Kamble
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Structural Damage ◽  
Pathogenic Bacteria ◽  
Vision Loss ◽  
Antibiotic Sensitivity ◽  
Human Beings ◽  
Corneal Ulcers ◽  
Sensitive Organ

Background: The human eye is one of the most remarkable sensory systems. Human beings gather most of the information about the external environment through their eyes and thus rely on sight more than on any other sense, with the eye being the most sensitive organ we have. Molecular characterization of Staphylococcus aureus from the cases of corneal ulcers.Methods: A total of 300 samples of corneal ulcers collected from various ophthalmology hospitals, government hospital and clinical laboratories of different places of Maharashtra, India. The Isolates were identified based on the colony morphology and biochemical reaction. The isolates were subjected for antibiotic sensitivity test and perform its molecular characterization.Results: In present study, 39 coagulase positive Staphylococcus aureus, pathogenic bacteria isolated from corneal ulcers.Conclusions: Staphylococcus aureus is one of the most significant pathogens in bacterial keratitis. Early diagnosis and prompt treatment are needed to minimize the possibility of permanent vision loss and reduce structural damage to the cornea.

scholarly journals PREVALENCE AND MOLECULAR CHARACTERIZATION OF blaCTX-M-15-PRODUCING PATHOGENIC GRAM-NEGATIVE BACTERIA FROM VARIOUS CLINICAL SAMPLES

2018 ◽  
Vol 11 (10) ◽  
pp. 386
Author(s):  
Sindhuja S ◽  
Sureshkumar Bt ◽  
Janaki S ◽  
Thenmozhi S
Keyword(s):  
Molecular Characterization ◽  
Pathogenic Bacteria ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli

Objective: The objective of this study was to describe the prevalence and molecular characterization of blaCTX-M-15-producing pathogenic Gram-negative bacteria from various clinical samples isolated from clinically suspected patients.Methods: In this study, clinical samples of urine, stool, sputum, and pus were collected from 244 patients with nosocomial infections. The phenotypic identification of extended-spectrum β-lactamases (ESBL) was confirmed by double-disk synergy test and combined disk diffusion test. In vitro, the susceptibility pattern of antimicrobial agents against pathogenic isolates was performed by Kirby–Bauer disk diffusion method. The identification of blaCTX-M-15-producing Escherichia coli was assessed by polymerase chain reaction method.Results: The frequency of ESBL-producing pathogenic bacteria from screened was 6 (46.15%). In vitro, susceptibility to pathogenic bacteria showed that the majority of isolates were highly susceptible to amoxicillin-clavulanic acid (97.87%), ofloxacin (93.33%), and Pseudomonas aeruginosa showed 100% sensitive to ceftazidime, cefotaxime, cefixime, cefoperazone, and meropenem (92.30%). The rates of resistance to other antibiotics varied from <26.66%. Among six tested isolates, only one E. coli isolates showed blaCTX-M-15 gene.Conclusion: Due to the increase of E. coli with multiple ESBL genes, continuous surveillance should be needed in clinical field to use of appropriate antibiotics and the control of infections.

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma

Molecular characterization of lincRNAs in Down syndrome associated leukemia

2012 ◽  
Vol 224 (03) ◽  
Author(s):  
A Streltsov ◽  
S Emmrich ◽  
F Engeland ◽  
JH Klusmann
Keyword(s):  
Down Syndrome ◽  
Molecular Characterization
Sign in / Sign up

Export Citation Format

Share Document