Capacity of bone marrow granylocyte and macrophage precursors in mice of different strains for in vitro colony formation under changed thymuline level in the organism and cell cultures

2017 ◽  
Vol XII (2) ◽  
Author(s):  
I. Labunets ◽  
A. Rodnichenko ◽  
R. Vasyliev
Keyword(s):  
Bone Marrow ◽  
Cell Cultures ◽  
Colony Formation ◽  
Different Strains ◽  
Macrophage Precursors

scholarly journals Interleukin 4 induces cultured monocytes/macrophages to form giant multinucleated cells.

1988 ◽  
Vol 167 (2) ◽  
pp. 598-611 ◽  
Author(s):  
A McInnes ◽  
D M Rennick
Keyword(s):  
Bone Marrow ◽  
Inflammatory Response ◽  
Cell Fusion ◽  
Cell Cultures ◽  
Soft Agar ◽  
Interleukin 4 ◽  
Multinucleated Cells ◽  
Granulomatous Lesions

Giant multinucleated cells (GMCs) are associated with granulomatous lesions that form in response to various infectious and noninfectious agents. The present study shows that mouse IL-4 induces the in vitro formation of GMCs by factor-dependent bone marrow and alveolar monocytes via cell fusion. GMCs appear 2 d after incubation of cell cultures with 20 U/ml or more of IL-4. Anti-IL-4 mAbs block the appearance of GMCs in these cultures, indicating that IL-4 acts directly on monocytes to promote fusion and does not secondarily induce the production of other soluble fusion factors. In soft agar cultures, IL-4 also causes the aggregation of macrophages and diminishes their migration. The role of IL-4 in a granulomatous inflammatory response is discussed.

scholarly journals In vitro improvement of bone marrow-derived hematopoietic colony formation in HIV-positive patients by alpha-D-tocopherol and erythropoietin

2009 ◽  
Vol 53 (4) ◽  
pp. 201-206 ◽  
Author(s):  
R. Georg Geissler ◽  
Arnold Ganser ◽  
Oliver G. Ottmann ◽  
Peter Gute ◽  
Anja Morawetz ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Hiv Positive

scholarly journals Serial in vitro bone marrow fibroblast culture in human leukemia

Blood ◽  
1983 ◽  
Vol 61 (3) ◽  
pp. 589-592 ◽  
Author(s):  
T Nagao ◽  
K Yamauchi ◽  
M Komatsuda
Keyword(s):  
Bone Marrow ◽  
Acute Leukemia ◽  
Colony Formation ◽  
Calf Serum ◽  
Culture Method ◽  
Fibroblast Culture ◽  
Human Leukemia ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia

Abstract Human fibroblast colony formation from bone marrow was performed in liquid culture. Fetal calf serum was used as a stimulator of the fibroblast colony formation. The colony formation took place not only in normal donors, but also in patients with acute leukemia and chronic myelocytic leukemia. At the diagnosis of the disease, significant colony suppression was observed in most cases of acute leukemia, while the number of colonies increased in half of the cases of chronic myelocytic leukemia. However, there was no correlation between the colony-forming efficiency and the initial number of peripheral platelets or bone marrow megakaryocytes that contained growth-promoting factor. The number of colonies increased after chemotherapy, recovered at the stage of complete remission, and then decreased to low levels at relapse in the patients with acute leukemia; it decreased after treatment with busulfan in the patients with chronic myelocytic leukemia. This fibroblast culture method is useful for counting fibroblast colony-forming cells in the bone marrow of human leukemia.

scholarly journals Enhancing and suppressing effects of recombinant murine macrophage inflammatory proteins on colony formation in vitro by bone marrow myeloid progenitor cells

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1110-1116 ◽  
Author(s):  
HE Broxmeyer ◽  
B Sherry ◽  
L Lu ◽  
S Cooper ◽  
KO Oh ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Colony Formation ◽  
Pokeweed Mitogen ◽  
Myeloid Progenitor ◽  
Hla Dr ◽  
Myeloid Progenitor Cells ◽  
Macrophage Colony Stimulating Factor ◽  
Inflammatory Proteins ◽  
Macrophage Colony

Abstract Purified recombinant (r) macrophage inflammatory proteins (MIPs) 1 alpha, 1 beta, and 2 were assessed for effects on murine (mu) and human (hu) marrow colony-forming unit-granulocyte-macrophage (CFU-GM) and burst-forming unit-erythroid (BFU-E) colonies. Recombinant MIP-1 alpha, -1 beta, and -2 enhanced muCFU-GM colonies above that stimulated with 10 to 100 U natural mu macrophage-colony-stimulating factor (M-CSF) or rmuGM-CSF, with enhancement seen on huCFU-GM colony formation stimulated with suboptimal rhuM-CSF or rhuGM-CSF; effects were neutralized by respective MIP-specific antibodies. Macrophage inflammatory proteins had no effects on mu or huBFU-E colonies stimulated with erythropoietin (Epo). However, natural MIP-1 and rMIP-1 alpha, but not rMIP-1 beta or -2, suppressed muCFU-GM stimulated with pokeweed mitogen spleen-conditioned medium (PWMSCM), huCFU-GM stimulated with optimal rhuGM-CSF plus rhu interleukin-3 (IL-3), muBFU- E and multipotential progenitors (CFU-GEMM) stimulated with Epo plus PWMSCM, and huBFU-E and CFU-GEMM stimulated with Epo plus rhuIL-3 or rhuGM-CSF. The suppressive effects of natural MIP-1 and rMIP-1 alpha were also apparent on a population of BFU-E, CFU-GEMM, and CFU-GM present in cell-sorted fractions of human bone marrow (CD34 HLA-DR+) highly enriched for progenitors with cloning efficiencies of 42% to 75%. These results, along with our previous studies, suggest that MIP-1 alpha, -1 beta, and -2 may have direct myelopoietic enhancing activity for mature progenitors, while MIP-1 alpha may have direct suppressing activity for more immature progenitors.

scholarly journals Phenotypic Characterization of Bone Marrow Mononuclear Cells and Derived Stromal Cell Populations from Human Iliac Crest, Vertebral Body and Femoral Head

2019 ◽  
Vol 20 (14) ◽  
pp. 3454 ◽  
Author(s):  
Marietta Herrmann ◽  
Maria Hildebrand ◽  
Ursula Menzel ◽  
Niamh Fahy ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Femoral Head ◽  
Cell Surface ◽  
Cell Cultures ◽  
Vertebral Body ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Iliac Crest ◽  
Surface Marker

(1) In vitro, bone marrow-derived stromal cells (BMSCs) demonstrate inter-donor phenotypic variability, which presents challenges for the development of regenerative therapies. Here, we investigated whether the frequency of putative BMSC sub-populations within the freshly isolated mononuclear cell fraction of bone marrow is phenotypically predictive for the in vitro derived stromal cell culture. (2) Vertebral body, iliac crest, and femoral head bone marrow were acquired from 33 patients (10 female and 23 male, age range 14–91). BMSC sub-populations were identified within freshly isolated mononuclear cell fractions based on cell-surface marker profiles. Stromal cells were expanded in monolayer on tissue culture plastic. Phenotypic assessment of in vitro derived cell cultures was performed by examining growth kinetics, chondrogenic, osteogenic, and adipogenic differentiation. (3) Gender, donor age, and anatomical site were neither predictive for the total yield nor the population doubling time of in vitro derived BMSC cultures. The abundance of freshly isolated progenitor sub-populations (CD45−CD34−CD73+, CD45−CD34−CD146+, NG2+CD146+) was not phenotypically predictive of derived stromal cell cultures in terms of growth kinetics nor plasticity. BMSCs derived from iliac crest and vertebral body bone marrow were more responsive to chondrogenic induction, forming superior cartilaginous tissue in vitro, compared to those isolated from femoral head. (4) The identification of discrete progenitor populations in bone marrow by current cell-surface marker profiling is not predictive for subsequently derived in vitro BMSC cultures. Overall, the iliac crest and the vertebral body offer a more reliable tissue source of stromal progenitor cells for cartilage repair strategies compared to femoral head.

Role of Erythropoietin in Megakaryocyte Colony Formation in Man

1979 ◽  
Author(s):  
W. Vainehenker ◽  
J. Breton-Gorius
Keyword(s):  
Bone Marrow ◽  
Linear Relationship ◽  
Colony Formation ◽  
Colony Stimulating Factor ◽  
Plasma Clot ◽  
In Vitro Technique ◽  
One Step ◽  
Stimulating Factor

We have recently realized megakaryocyte (MK) colony formation in culture from blood and bone marrow progenitors using the plasma clot technique. In this study, the MK stimulating factor was an erythropoietin (Epo) either a poorly purified one(step III from anaemic sheep serum, a crude serum from anaemic mice, an urinary human Epo) or a highly purified one (GOLDWASSER). Similar results were obtained with all these Epo. A linear relationship was found between the number of colonies and seeded cells. However with less than 5.105 plated cells from the blood, no MK colonies were obtained, although erythroid colonies could be grown. In contrast, without Epo, spontaneous colonies could be observed which represented 1/5 th of the maximum plating efficiency , in these eases no erythroid colonies were present. These data suggest that Epo itself acts an a MK colony stimulating factor; but is not the only factor involved in the formation of MK colonies. This in vitro technique will be useful of in determining the factors regulating megakaryocytopoiesis.

Sign in / Sign up

Export Citation Format

Share Document