scholarly journals Genetic Characterization of Juvenile Chum Salmon (Oncorhynchus keta) Migrating out of the Yukon River Delta

2019 ◽  
pp. 51-53
Author(s):  
Genevieve Johnson ◽  
Christine Kondzela ◽  
Jacqueline Whittle ◽  
Katharine Miller ◽  
Jeffrey Guyon
Keyword(s):  
Chum Salmon ◽  
Genetic Characterization ◽  
River Delta ◽  
Oncorhynchus Keta ◽  
Chum Salmon Oncorhynchus Keta ◽  
Yukon River ◽  
Juvenile Chum Salmon

scholarly journals Neural Stem Cells/Neuronal Precursor Cells and Postmitotic Neuroblasts in Constitutive Neurogenesis and After ,Traumatic Injury to the Mesencephalic Tegmentum of Juvenile Chum Salmon, Oncorhynchus keta

2020 ◽  
Vol 10 (2) ◽  
pp. 65
Author(s):  
Pushchina ◽  
Kapustyanov ◽  
Varaksin
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Progenitor Cells ◽  
Chum Salmon ◽  
Precursor Cells ◽  
Oncorhynchus Keta ◽  
Neuronal Precursor ◽  
Chum Salmon Oncorhynchus Keta ◽  
Neuronal Precursor Cells ◽  
Juvenile Chum Salmon

The proliferation of neural stem cells (NSCs)/neuronal precursor cells (NPCs) and the occurrence of postmitotic neuroblasts in the mesencephalic tegmentum of intact juvenile chum salmon, Oncorhynchus keta, and at 3 days after a tegmental injury, were studied by immunohistochemical labeling. BrdU+ constitutive progenitor cells located both in the periventricular matrix zone and in deeper subventricular and parenchymal layers of the brain are revealed in the tegmentum of juvenile chum salmon. As a result of traumatic damage to the tegmentum, the proliferation of resident progenitor cells of the neuroepithelial type increases. Nestin-positive and vimentin-positive NPCs and granules located in the periventricular and subventricular matrix zones, as well as in the parenchymal regions of the tegmentum, are revealed in the mesencephalic tegmentum of juvenile chum salmon, which indicates a high level of constructive metabolism and constitutive neurogenesis. The expression of vimentin and nestin in the extracellular space, as well as additionally in the NSCs and NPCs of the neuroepithelial phenotype, which do not express nestin in the control animals, is enhanced during the traumatic process. As a result of the proliferation of such cells in the post-traumatic period, local Nes+ and Vim+ NPCs clusters are formed and become involved in the reparative response. Along with the primary traumatic lesion, which coincides with the injury zone, additional Nes+ and Vim+ secondary lesions are observed to form in the adjacent subventricular and parenchymal zones of the tegmentum. In the lateral tegmentum, the number of doublecortin-positive cells is higher compared to that in the medial tegmentum, which determines the different intensities and rates of neuronal differentiation in the sensory and motor regions of the tegmentum, respectively. In periventricular regions remote from the injury, the expression of doublecortin in single cells and their groups significantly increases compared to that in the damage zone.

Isolation and Characterization of Ten Novel Microsatellite Loci in Chum Salmon (Oncorhynchus keta)

2019 ◽  
Vol 45 (6) ◽  
pp. 491-493
Author(s):  
Ye Han ◽  
ShuFang Liu ◽  
JiWu Wan ◽  
ChunMei Yan ◽  
ZhongQiang Li ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Chum Salmon ◽  
Oncorhynchus Keta ◽  
Chum Salmon Oncorhynchus Keta ◽  
Isolation And Characterization

Size Selective Predation by Juvenile Chum Salmon (Oncorhynchus keta) on Epibenthic Prey in Puget Sound

1975 ◽  
Vol 32 (8) ◽  
pp. 1419-1429 ◽  
Author(s):  
Robert J. Feller ◽  
Victor W. Kaczynski
Keyword(s):  
Chum Salmon ◽  
Prey Selection ◽  
Puget Sound ◽  
Harpacticoid Copepod ◽  
Gut Contents ◽  
Oncorhynchus Keta ◽  
Size Spectrum ◽  
Natural Diet ◽  
Chum Salmon Oncorhynchus Keta ◽  
Juvenile Chum Salmon

Analysis of gut contents shows that juvenile (30–50 mm) chum salmon (Oncorhynchus keta) in Puget Sound select epibenthic organisms as their primary prey. Harpacticoid copepods numerically comprised over 80% of their natural diet in two areas studied, while terrestrial insects and cladocerans were most important in a third area. Calculation of Ivlev (1961) electivity coefficients indicated high selectivity factors for harpacticoids at one site (+0.59 to +0.94). Comparison of fish gut contents with quantitative epibenthic pump samples of available prey shows that prey selection was size related, but opposite that currently reported in the literature (e.g. Brooks and Dodson 1965); that is, the smaller of the available prey was preferred. This was true for both the total available prey size spectrum and the harpacticoid copepod fraction of the prey spectrum. Large numbers of prey eaten per fish suggest that juvenile chum salmon may exert high predation pressure on nearshore epibenthic organisms in Puget Sound during spring.

Isolation and characterization of L-rhamnose-binding lectins from chum salmon (Oncorhynchus keta) eggs

2002 ◽  
Vol 68 (6) ◽  
pp. 1352-1366 ◽  
Author(s):  
NOBUYUKI SHIINA ◽  
HIROAKI TATENO ◽  
TOMOHISA OGAWA ◽  
KOJI MURAMOTO ◽  
MINEO SANEYOSHI ◽  
...  
Keyword(s):  
Chum Salmon ◽  
Oncorhynchus Keta ◽  
Chum Salmon Oncorhynchus Keta ◽  
Isolation And Characterization

scholarly journals A Confocal Microscopic Study of Gene Transfer into the Mesencephalic Tegmentum of Juvenile Chum Salmon, Oncorhynchus keta, Using Mouse Adeno-Associated Viral Vectors

2021 ◽  
Vol 22 (11) ◽  
pp. 5661
Author(s):  
Evgeniya V. Pushchina ◽  
Ilya A. Kapustyanov ◽  
Ekaterina V. Shamshurina ◽  
Anatoly A. Varaksin
Keyword(s):  
Reticular Formation ◽  
Viral Vectors ◽  
Chum Salmon ◽  
Mesencephalic Reticular Formation ◽  
Laser Scanning Microscopy ◽  
Oncorhynchus Keta ◽  
Brain Cells ◽  
Nuclear Staining ◽  
Chum Salmon Oncorhynchus Keta ◽  
Juvenile Chum Salmon

To date, data on the presence of adenoviral receptors in fish are very limited. In the present work, we used mouse recombinant adeno-associated viral vectors (rAAV) with a calcium indicator of the latest generation GCaMP6m that are usually applied for the dorsal hippocampus of mice but were not previously used for gene delivery into fish brain. The aim of our work was to study the feasibility of transduction of rAAV in the mouse hippocampus into brain cells of juvenile chum salmon and subsequent determination of the phenotype of rAAV-labeled cells by confocal laser scanning microscopy (CLSM). Delivery of the gene in vivo was carried out by intracranial injection of a GCaMP6m-GFP-containing vector directly into the mesencephalic tegmentum region of juvenile (one-year-old) chum salmon, Oncorhynchus keta. AAV incorporation into brain cells of the juvenile chum salmon was assessed at 1 week after a single injection of the vector. AAV expression in various areas of the thalamus, pretectum, posterior-tuberal region, postcommissural region, medial and lateral regions of the tegmentum, and mesencephalic reticular formation of juvenile O. keta was evaluated using CLSM followed by immunohistochemical analysis of the localization of the neuron-specific calcium binding protein HuCD in combination with nuclear staining with DAPI. The results of the analysis showed partial colocalization of cells expressing GCaMP6m-GFP with red fluorescent HuCD protein. Thus, cells of the thalamus, posterior tuberal region, mesencephalic tegmentum, cells of the accessory visual system, mesencephalic reticular formation, hypothalamus, and postcommissural region of the mesencephalon of juvenile chum salmon expressing GCaMP6m-GFP were attributed to the neuron-specific line of chum salmon brain cells, which indicates the ability of hippocampal mammal rAAV to integrate into neurons of the central nervous system of fish with subsequent expression of viral proteins, which obviously indicates the neuronal expression of a mammalian adenoviral receptor homolog by juvenile chum salmon neurons.

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