scholarly journals WNT5A Expression in Ameloblastoma and Its Roles in Regulating Enamel Epithelium Tumorigenic Behaviors

2010 ◽  
Vol 176 (1) ◽  
pp. 461-471 ◽  
Author(s):  
Waleerat Sukarawan ◽  
Darrin Simmons ◽  
Cynthia Suggs ◽  
Kimberly Long ◽  
J. Timothy Wright
Keyword(s):  
Wnt5a Expression ◽  
Enamel Epithelium

Opg, Rank, and Rankl in Tooth Development: Co-ordination of Odontogenesis and Osteogenesis

2004 ◽  
Vol 83 (3) ◽  
pp. 241-244 ◽  
Author(s):  
A. Ohazama ◽  
J.-M. Courtney ◽  
P.T. Sharpe
Keyword(s):  
Tooth Development ◽  
Nuclear Factor Κb ◽  
Cellular Interactions ◽  
Rankl Expression ◽  
Dental Papilla ◽  
Spatiotemporal Expression ◽  
Receptor Activator ◽  
Explant Cultures ◽  
Enamel Epithelium ◽  
Tooth Germs

Osteoprotegerin (OPG), receptor activator of nuclear factor-κB (RANK), and RANK ligand (RANKL) are mediators of various cellular interactions, including bone metabolism. We analyzed expression of these three genes during murine odontogenesis from epithelial thickening to cytodifferentiation stages. Opg showed expression in the thickening and bud epithelium. Expression of Opg and Rank was observed in both the internal and the external enamel epithelium as well as in the dental papilla mesenchyme. Although Rankl expression was not detected in tooth epithelium or mesenchyme, it was expressed in pre-osteogenic mesenchymal cells close to developing tooth germs. All three genes were detected in developing dentary bone at P0. The addition of exogenous OPG to explant cultures of tooth primordia produced a delay in tooth development that resulted in reduced mineralization. We propose that the spatiotemporal expression of these molecules in early tooth and bone primordia cells has a role in co-ordinating bone and tooth development.

An Analysis of WNT5A expression in Wilms’ Tumour

2018 ◽  
Author(s):  
Nicholas Laughton
Keyword(s):  
Messenger Rna ◽  
Published Data ◽  
Expression Data ◽  
Wilms Tumour ◽  
Chain Reaction ◽  
Wnt5a Expression ◽  
Extracellular Signal ◽  
Developing Kidney ◽  
Polymerase Chain ◽  
Pax2 Expression

Wilms’ tumour is a pediatric tumour of the kidney that appears to be the result of abherrent embryonal renal development. The paired­box (PAX) gene family has previously been implicated in Wilm’s tumorogenesis. In this study, Nickel­Agarose Chromatin Enrichment (NACE) was used to identify genes whose expression is regulated by the ranscription cofactor Pax2. Of the genes identified by NACE, the extracellular signal metabolite WNT5A was chosen fro further study. The expression of WNT5A was measured in a set of tumour samples using quantitative real time polymerase chain reaction (qRT­PCR) and compared to a human fetal kidney control. Of the 38 samples tested, 76% showed significantly lower levelsof cytosolic messenger RNA (mRNA). This data, in conjunction with published data on Pax2 expression, suggests Pax2 inhibits the expression of WNT5A. When compared with histological reports for the tumours we examined, the expression data implies that WNT5A may have a role in regulation of tubule growth in the developing kidney

Lesions of the Enamel Organ of Developing Dog Teeth following Experimental Inoculation of Gnotobiotic Puppies with Canine Distemper Virus

1981 ◽  
Vol 18 (5) ◽  
pp. 684-689 ◽  
Author(s):  
R. R. Dubielzig ◽  
R. J. Higgins ◽  
S. Krakowka
Keyword(s):  
Canine Distemper Virus ◽  
Giant Cells ◽  
Enamel Organ ◽  
Post Inoculation ◽  
Canine Distemper ◽  
Experimental Inoculation ◽  
Enamel Epithelium ◽  
Viral Inclusions ◽  
Reduced Enamel Epithelium ◽  
Multinucleate Giant Cells

Ten 7-day-old gnotobiotic Beagle puppies were inoculated intraperitoneally with virulent canine distemper virus (R252-CDV). The dogs were killed and perfused with paraformaldehyde/glutaraldehyde from eight to 36 days after inoculation. The developing teeth of the mandibles were examined by light microscopy, and the teeth from three dogs were examined by electron microscopy. Necrosis of individual cells in the stratum intermedium of the developing tooth was the first change, detectable at day 9 post-inoculation. At day 16 post-inoculation, there was disorganization of the ameloblasts. In the stratum intermedium, multinucleate giant cells and large eosinophilic cytoplasmic viral inclusions were prominent. Ultrastructurally, these inclusions consisted of clusters of tubular aggregates typical of canine distemper virus nucleocapsids. At 28 to 36 days post-inoculation, the changes were seen in the reduced enamel epithelium. Multinucleate cells were seen, but no inclusions. Some necrotic cells were seen. In these teeth, ameloblastic cells of the root were morphologically normal. Our results suggest that distemper virus affects developing teeth by direct infection of the enamel organ.

scholarly journals Clinical Importance of Wnt5a in the Pathogenesis of Colorectal Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Mehran Pashirzad ◽  
Thozhukat Sathyapalan ◽  
Amirhossein Sahebkar
Keyword(s):  
Colorectal Cancer ◽  
Molecular Mechanisms ◽  
Potential Role ◽  
Clinical Importance ◽  
Signaling Cascades ◽  
Cancer Type ◽  
Invasion And Metastasis ◽  
Cellular Processes ◽  
Wnt5a Expression ◽  
Noncanonical Signaling

Wnt5a is one of the potent signaling molecules that initiates responses involved in cancer through activation of both canonical and noncanonical signaling cascades. Wnt5a both directly and indirectly triggers cancer-associated signaling pathways based on the cancer type. In colorectal cancer (CRC), altering Wnt5a expression can influence several cellular processes of tumor cells, including proliferation, differentiation, migration, invasion, and metastasis. This review summarizes the molecular mechanisms and clinical importance of Wnt5a in the pathogenesis of CRC for better understanding the pathogenesis and its potential role as a prognostic marker and as an appropriate therapeutic target in the treatment of this disease in the future.

scholarly journals PITPNA-AS1 abrogates the inhibition of miR-876-5p on WNT5A to facilitate hepatocellular carcinoma progression

2019 ◽  
Vol 10 (11) ◽  
Author(s):  
Jianmin Sun ◽  
Yubao Zhang ◽  
Bing Li ◽  
Yuandi Dong ◽  
Chengming Sun ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Treatment Strategies ◽  
Diagnostic Biomarkers ◽  
Cellular Processes ◽  
Candidate Mirnas ◽  
In Vivo Experiments ◽  
Wnt5a Expression ◽  
Hep3b Cells ◽  
Hcc Cells

Abstract LncRNA PITPNA-AS1 was a newly identified lncRNA which has never been studied in cancers. Whether PITPNA-AS1 participated in the development of hepatocellular carcinoma (HCC) is obscure. Given the coaction of lncRNAs and miRNAs to carcinogenesis, the purpose of the present research is to inquire how PITPNA-AS1 affects HCC progression. Firstly, PITPNA-AS1 was observed to be heightened in HCC tissues. Then function assays proved that overexpressing or silencing PITPNA-AS1 could manipulate the proliferation and motility of HCC cells. Besides, PITPNA-AS1 was located in the cytoplasm. Among the candidate miRNAs of PITPNA-AS1, miR-876-5p was an obvious target. Moreover, mechanism experiments validated that PITPNA-AS1 modulated WNT5A expression by targeting miR-876-5p. Rescue experiments affirmed that WNT5A silencing rescued the miR-876-5p suppression-induced cellular processes in PITPNA-AS1-silenced Hep3B cells. And in vivo experiments determined that PITPNA-AS1 regulated HCC progression in vivo via miR-876-5p/WNT5A pathway. In conclusion, this work shed lights on the modulatory mechanism of PITPNA-AS1/miR-876-5p/WNT5A axis in HCC, which might be pivotal for exploring effective diagnostic biomarkers and treatment strategies for HCC patients.

scholarly journals Pericoronal Follicles of Asymptomatic Impacted Teeth: A Radiographic, Histomorphologic, and Immunohistochemical Study

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Laura Villalba ◽  
Federico Stolbizer ◽  
Fabián Blasco ◽  
Néstor Raúl Mauriño ◽  
María Julia Piloni ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Immunohistochemical Study ◽  
Squamous Metaplasia ◽  
Ki 67 ◽  
Impacted Teeth ◽  
Positive Expression ◽  
Dentigerous Cysts ◽  
Proliferation And Apoptosis ◽  
Enamel Epithelium ◽  
Reduced Enamel Epithelium

Objective. To associate radiographic and histopathological features of pericoronal follicles (PFs) of asymptomatic impacted teeth and evaluate cell proliferation and apoptosis in the epithelium.Study Design. Epithelium and mesenchyme of radiographically normal (NPF≤2.5 mm) and hyperplastic (HPF 2.6 to 5 mm) PF (n=140) were studied histologically. Cell proliferation (PI) and epithelial apoptosis were evaluated by Ki-67 and bcl-2 expression in 14 NPFs and 10 dentigerous cysts (DCs).Results. Radiographically, 127 were NPFs and 13 were HPFs; 87.8% of total PFs exhibited epithelium on the surface. Reduced enamel epithelium was observed in 78 (61.4%) NPFs and 6 (46.2%) HPFs, squamous metaplasia in 17 (13.4%) NPFs and 4 (30.8%) HPFs, and cystic epithelium in 15 (11.8%) NPFs and 3 (23%) HPFs. Mean PI was1.97±1.25and7.97±1.74in the epithelial component of NPF and DC, respectively; bcl-2 positive expression was observed in 9 (64.3%) NPFs and 7 (70%) DCs.Conclusion. The scant epithelial remnant proliferation could imply low risk for development of odontogenic pathologies in the absence of an additional stimulus.

scholarly journals Wnt5a expression and prognosis in stage II–III colon cancer

2021 ◽  
Vol 14 (1) ◽  
pp. 100892
Author(s):  
Cecilia Margareta Lund ◽  
Anne Dyhl-Polk ◽  
Dorte Lisbeth Nielsen ◽  
Lene Buhl Riis
Keyword(s):  
Colon Cancer ◽  
Stage Ii ◽  
Wnt5a Expression
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