scholarly journals Prognostic Significance of Stromal Platelet-Derived Growth Factor β-Receptor Expression in Human Breast Cancer

2009 ◽  
Vol 175 (1) ◽  
pp. 334-341 ◽  
Author(s):  
Janna Paulsson ◽  
Tobias Sjöblom ◽  
Patrick Micke ◽  
Fredrik Pontén ◽  
Göran Landberg ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Human Breast Cancer ◽  
Prognostic Significance ◽  
Receptor Expression ◽  
Platelet Derived Growth Factor ◽  
Human Breast ◽  
Β Receptor

scholarly journals Mechanism involved in genistein activation of insulin-like growth factor 1 receptor expression in human breast cancer cells

2007 ◽  
Vol 98 (6) ◽  
pp. 1120-1125 ◽  
Author(s):  
Wen-Fang Chen ◽  
Quan-Gui Gao ◽  
Man-Sau Wong
Keyword(s):  
Breast Cancer ◽  
Growth Factor ◽  
Receptor Antagonist ◽  
Human Breast Cancer ◽  
Promoter Activity ◽  
Receptor Expression ◽  
Receptor Protein ◽  
Insulin Like Growth Factor ◽  
Human Breast ◽  
Mcf 7

Our previous studies have shown that genistein can enhance the insulin-like growth factor (IGF)-1 receptor signalling pathway via an oestrogen receptor (ER) in human breast cancer MCF-7 cells. The present study aims to investigate how genistein regulates IGF-1 receptor expression in human MCF-7 cells. Genistein at 1 μm stimulated the growth of MCF-7 cells and this effect could be completely blocked by the IGF-1 receptor antagonist JB-1, suggesting that IGF-1 receptor is essential for mediating the proliferative effects of genistein in MCF-7 cells. Genistein increased IGF-1 receptor promoter activity. This effect could be completely abolished by co-treatment of MCF-7 cells with ICI 182,780 (10− 6 m). Genistein increased IGF-1 receptor gene expression and this effect could be completely blocked by the IGF-1 receptor antagonist JB-1. Co-treatment of MCF-7 cells with cycloheximide (5 μg/ml) completely blocked the induction of IGF-1 receptor protein and mRNA expression by genistein. The results indicated that the induction of IGF-1 receptor promoter activity by genistein required the action of ER while the stimulatory actions of genistein on IGF-1 receptor expression required the activity of the IGF-1 receptor and de novo protein synthesis. These data provide evidence to support the hypothesis that the inductive effects of genistein on IGF-1 receptor expression require the cross-talk between IGF-1 receptor and the ER-dependent pathways.

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