scholarly journals Responses of Nontransformed Human Hepatocytes to Conditional Expression of Full-Length Hepatitis C Virus Open Reading Frame

2007 ◽  
Vol 171 (6) ◽  
pp. 1831-1846 ◽  
Author(s):  
Weiliang Tang ◽  
Catherine A. Lázaro ◽  
Jean S. Campbell ◽  
W. Tony Parks ◽  
Michael G. Katze ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Hepatocytes ◽  
Full Length ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Conditional Expression

scholarly journals Generation of Infectious Hepatitis C Virus in Immortalized Human Hepatocytes

2006 ◽  
Vol 80 (9) ◽  
pp. 4633-4639 ◽  
Author(s):  
Tatsuo Kanda ◽  
Arnab Basu ◽  
Robert Steele ◽  
Takaji Wakita ◽  
Jan S. Ryerse ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Protein Expression ◽  
Culture Media ◽  
Human Hepatocytes ◽  
Full Length ◽  
Dependent Manner ◽  
Virus Growth ◽  
Hcv Genotype

ABSTRACT Progress in understanding hepatitis C virus (HCV) biology has remained a challenge due to the lack of an efficient cell culture system for virus growth. In this study, we examined HCV core protein-mediated immortalized human hepatocytes (IHH) for growth of HCV. In vitro-transcribed full-length RNA from HCV genotype 1a (clone H77) was introduced into IHH by electroporation. Reverse transcription-PCR of cellular RNA isolated from HCV genome-transfected IHH suggested that viral RNA replication occurred. IHH transfected with the full-length HCV genome also displayed viral protein expression by indirect immunofluorescence. In contrast, cells transfected with polymerase-defective HCV (H77/GND) RNA as a negative control did not exhibit expression of the viral genome. Immunogold labeling demonstrated localization of E1 protein in the rough endoplasmic reticulum of RNA-transfected IHH. Virus-like particles of ∼50 nm were observed in the cytoplasm. After being inoculated with culture media of cells transfected with the full-length HCV genome, naïve IHH displayed NS5a protein expression in a dilution-dependent manner, but expression of NS5a was inhibited by prior incubation of culture medium with HCV-infected patient sera. NS5a-positive immunofluorescence of cell culture media of IHH transfected with full-length H77 RNA yielded ∼4.5 × 104 to 1 × 105 focus-forming units/ml. A similar level of virus growth was observed upon transfection of RNA from HCV genotype 2a (JFH1) into IHH. Taken together, our results suggest that IHH support HCV genome replication and virus assembly.

scholarly journals Expression of the Novel Hepatitis C Virus Core+1/ARF Protein in the Context of JFH1-Based Replicons

2015 ◽  
Vol 89 (9) ◽  
pp. 5164-5170 ◽  
Author(s):  
Ioly Kotta-Loizou ◽  
Ioannis Karakasiliotis ◽  
Niki Vassilaki ◽  
Panagiotis Sakellariou ◽  
Ralf Bartenschlager ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Gene ◽  
Open Reading Frame ◽  
The Novel ◽  
Reading Frame ◽  
The Core ◽  
Virus Core ◽  
First Time ◽  
Arf Protein

Hepatitis C virus contains a second open reading frame within the core gene, designated core+1/ARF. Here we demonstrate for the first time expression of core+1/ARF protein in the context of a bicistronic JFH1-based replicon and report the production of two isoforms, core+1/L (long) and core+1/S (short), with different kinetics.

scholarly journals Expression profiling of liver cell lines expressing entire or parts of hepatitis C virus open reading frame

Hepatology ◽  
2002 ◽  
Vol 36 (6) ◽  
pp. 1431-1438 ◽  
Author(s):  
Hideki Aizaki ◽  
Takashi Harada ◽  
Motoyuki Otsuka ◽  
Naohiko Seki ◽  
Mami Matsuda ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Lines ◽  
Liver Cell ◽  
Expression Profiling ◽  
Open Reading Frame ◽  
Reading Frame

scholarly journals Genome Sequence of an Unknown Subtype of Hepatitis C Virus Genotype 6: Another Piece for the Taxonomic Puzzle

2019 ◽  
Vol 8 (42) ◽  
Author(s):  
Martin Schou Pedersen ◽  
Sarah Mollerup ◽  
Lone Gilmor Nielsen ◽  
Håvard Jenssen ◽  
Jens Bukh ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Genome Sequence ◽  
Open Reading Frame ◽  
Virus Genotype ◽  
Reading Frame ◽  
Open Reading Frame Sequence ◽  
Frame Sequence ◽  
Reference Genomes ◽  
Virus Strains

The surveillance and correct subtyping of hepatitis C virus strains require available and up-to-date publicly available reference genomes. Here, we present the complete open reading frame sequence of a hepatitis C virus genotype 6 strain of an unknown subtype that was discovered during routine subtyping of patients in the clinic.

scholarly journals Hepatitis C Virus Diversity and Evolution in the Full Open-Reading Frame during Antiviral Therapy

PLoS ONE ◽  
2008 ◽  
Vol 3 (5) ◽  
pp. e2123 ◽  
Author(s):  
Nathan A. Cannon ◽  
Maureen J. Donlin ◽  
Xiaofeng Fan ◽  
Rajeev Aurora ◽  
John E. Tavis ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Antiviral Therapy ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Virus Diversity

scholarly journals Ribavirin-induced mutagenesis across the complete open reading frame of hepatitis C virus genotypes 1a and 3a

2018 ◽  
Vol 99 (8) ◽  
pp. 1066-1077 ◽  
Author(s):  
Niels Mejer ◽  
Ulrik Fahnøe ◽  
Andrea Galli ◽  
Santseharay Ramirez ◽  
Thomas Benfield ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Complete Open Reading Frame ◽  
Virus Genotypes ◽  
Induced Mutagenesis ◽  
Hepatitis C Virus Genotypes
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