scholarly journals Effect of Bioactive Primers on Bacterial-Induced Secondary Caries at the Tooth-Resin Interface

2017 ◽  
Vol 42 (2) ◽  
pp. 196-202 ◽  
Author(s):  
GE Kim ◽  
AA Leme-Kraus ◽  
R Phansalkar ◽  
G Viana ◽  
C Wu ◽  
...  

SUMMARY Secondary caries at the tooth-resin interface is the primary reason for replacement of resin composite restorations. The tooth-resin interface is formed by the interlocking of resin material with hydroxyapatite crystals in enamel and collagen mesh structure in dentin. Efforts to strengthen the tooth-resin interface have identified chemical agents with dentin collagen cross-linking potential and antimicrobial activities. The purpose of the present study was to assess protective effects of bioactive primer against secondary caries development around enamel and dentin margins of class V restorations, using an in vitro bacterial caries model. Class V composite restorations were prepared on 60 bovine teeth (n=15) with pretreatment of the cavity walls with control buffer solution, an enriched fraction of grape seed extract (e-GSE), 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimide/N-hydroxysuccinimide, or chlorhexidine digluconate. After incubating specimens in a bacterial model with Streptococcus mutans for four days, dentin and enamel were assessed by fluorescence microscopy. Results revealed that only the naturally occurring product, e-GSE, significantly inhibited the development of secondary caries immediately adjacent to the dentin-resin interface, as indicated by the caries inhibition zone. No inhibitory effects were observed in enamel margins. The results suggest that the incorporation of e-GSE into components of the adhesive system may inhibit secondary caries and potentially contribute to the protection of highly vulnerable dentin-resin margins.

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Morakot Piemjai ◽  
Pareewan Chantarawej ◽  
Nobuo Nakabayashi

Purpose. Secondary caries originate from a leakage pathway where oral acids can penetrate faster and demineralize the tooth substrate deeper which can be visualized by dye penetration. The ability to prevent secondary caries by contemporary adhesive systems was evaluated in this study. Dye penetration distance through leakage and into the tooth substrate adjacent to Class V restorations after artificial caries exposure was compared. Materials and Methods. Previously frozen extracted human molars were used to prepare the Class V cavities at the CEJ on axial surfaces. All cavities were restored with either the resin-composite or amalgam with or without resin adhesives: dry bonding: Super-Bond D-Liner II Plus; moist bonding: All-Bond 2; and self-etch bonding: AQ Bond and Clearfil Protect Bond. Two subgroups of Super-Bond D-Liner II Plus were immersed for 14 days at 37°C either in artificial saliva (negative control) or the artificial caries solution. The other groups were soaked in the artificial caries solution. The distance of dye penetration into the adjacent enamel, cementum/dentin, and tooth-resin interfaces was measured after immersion in 0.5% basic fuchsin dye for 24 h. The tooth-resin interfacial layer was investigated using SEM. Results. No dye penetration into the tooth-resin interface was found in Super-Bond D-Liner II Plus and AQ Bond groups which demonstrated a constant hybrid layer after a chemical challenge. The leakage distance at the cementum/dentin-resin interface of All-Bond 2, Clearfil Protect Bond, and non-adhesive amalgam (positive control) groups was significantly higher than the distance of dye penetration into the adjacent demineralized root surface (p<0.05). Conclusion. Caries associated with either amalgam or resin-composite restorations can be prevented using resin adhesives which can penetrate into the intact tooth substrate to form a stable hybrid layer. With caries-free restorations, tooth vitality may be conserved lifelong.


2016 ◽  
Vol 63 (1) ◽  
pp. 15-21 ◽  
Author(s):  
Navjot S. Mann ◽  
Sameer Makkar ◽  
Reetika Sharma

SUMMARY Introduction The aim of this study was to evaluate marginal sealing ability of newly introduced Dyad Flow flowable composite and compare to total-etch and self-etch adhesive system at the coronal and apical margins of class V resin composite restorations. Material and Methods A standard class V cavity (3 mm mesiodistal width, 3 mm occlusogingival height and 1.5 mm axial depth) was prepared on the buccal surface of freshly extracted sound human teeth. Occlusal and gingival margins of the cavities were located in enamel and cementum/dentin, respectively. Teeth were randomly assigned into the three groups (n=10) and restored with different composite materials following the manufacturer’s instructions: group I was restored with nanohybrid resin composite using total-etch bonding agent; group II was restored with nanohybrid resin composite using self-etch technique; group III was restored with flowable composite (Dyad Flow), respectively. After finishing and polishing, the teeth were coated with nail varnish and immersed in rhodamine B dye and sectioned longitudinally. Dye penetration was examined under stereomicroscope and scored separately for occlusal and gingival margins on a 0–3 ordinal scale. Data were analyzed with Kruskal–Wallis, Mann–Whitney and Wilcoxon tests (a=0.05). Result Statistical analysis showed that specimens restored with the total-etch and self-etch adhesive systems revealed reduced leakage at the coronal margin. At the apical margin, Dyad Flow showed greater leakage than other groups. Conclusion Newly introduced flowable composite Dyad Flow showed inferior adhesive bond with enamel and dentin compared to total-etch and self-etch techniques.


2021 ◽  
Vol 20 ◽  
pp. e210525
Author(s):  
Shahin Kasraei ◽  
Sogol NejadKarimi ◽  
Mona Malek ◽  
Mahsa Mohammadi

Aim: Recent reports indicate that deproteinization of acid-etched dentin surface can extend penetration depth of adhesive agents. The main goal of the present research was to investigate the deproteinization effect of Nd:YAG and diode 940 lasers on acid-etched dentin and microleakage grade in class V composite restorations. Methods: 36 extracted human premolar teeth were selected to make standard buccal and lingual class V cavities. These samples were randomly split into three sub-groups: 1.Control group, in which composite was applied for restoration after etch and bonding process without deproteinization; 2.Nd:YAG laser group, in which the teeth were deproteinized with Nd:YAG laser after etching and painting internal surfaces of cavities with Van Geison stain and then composite restorations applied just as control group; 3.Diode laser group, in which the process was similar to Nd:YAG laser group, but instead, diode 940 laser was irradiated. The teeth were bisected into two equal longitudinal buccal and lingual halves. Marginal microleakage of samples was scored by using a stereomicroscope. Kruskal- Wallis, Mann-Whitney U and Fisher’s statistical tests were employed for analysis of the obtained data. Results: A significant reduction in marginal microleakage was observed for both groups treated with laser (Nd:YAG and diode 940)compared to control (p=0.001 & p=0.047). There was no significant difference in marginal microleakage between Nd:YAG laser and diode 940groups (P = 0.333). Conclusion: Nd:YAG and diode 940 laser deproteinization of acid-etched dentin decreased the marginal microleakage of in-vitro class V resin composite restorations.


2006 ◽  
Vol 31 (6) ◽  
pp. 688-693 ◽  
Author(s):  
B. A. C. Loomans ◽  
N. J. M. Opdam ◽  
F. J. M. Roeters ◽  
E. M. Bronkhorst ◽  
R. C. W. Burgersdijk

Clinical Relevance When placing a Class II resin composite restoration, the use of sectional matrix systems and separation rings to obtain tight proximal contacts is recommended.


2019 ◽  
Vol 10 (4) ◽  
pp. 275-282
Author(s):  
Niusha Golbar ◽  
Shahin Kasraei ◽  
Anahit Afrasiabi ◽  
Elahe Mostajir ◽  
Seyed Masoud Mojahedi

Introduction: Some studies have shown that laser irradiation on unpolymerized adhesives can improve composite-dentin adhesion. The aim of the present study was to evaluate the effect of the diode laser (810 nm) on the microleakage of multi-mode adhesive systems at enamel and dentin margins of composite restorations. Methods: Classic class V boxes were prepared on 48 sound premolar teeth and randomly divided into 6 groups (n=16). In the control groups, Scotchbond Universal (SBC), G-Premio (GBC), and Ambar U (AMC) were used by a self-etch mode. In the test groups (SBL, GBL, ABL), the 810 nm diode laser was irradiated (1 W) for 10 seconds before the polymerization of the adhesive. The boxes were restored by the resin composite. After finishing and polishing, the samples were thermocycled (5°C to 55°C) for 1000 cycles and then immersed in 0.1% methylene blue dye (48 hours). Dye penetration through the gingival and occlusal margins was measured by Stereomicroscope. The data were analyzed at the 5% significance level using Kruskal-Wallis and Mann-Whitney U tests. Results: Significant differences were found between the control and test groups (P<0.05). The occlusal margins of the SBL and GBL groups and the cervical margin of the SBL group exhibited the lowest microleakage (P<0.05). The AM control group showed maximum microleakage at cervical and occlusal margins. Conclusion: The irradiation of the 810 nm diode laser on the unpolymerized universal adhesive systems in a self-etch mode caused a significant reduction in enamel and dentin marginal microleakage of composite restorations.


2006 ◽  
Vol 31 (1) ◽  
pp. 60-67 ◽  
Author(s):  
B. M. Owens ◽  
W. W. Johnson ◽  
E. F. Harris

Clinical Relevance Decreased leakage was associated with Adper Scotchbond Multi-Purpose (total etch) and iBond (self-etch) systems in Class V resin composite restorations. All adhesive systems performed best when bonded to enamel compared to dentin surface anatomy. Clinicians should be aware that strict adherence to manufacturer instructions, when using these materials, is of primary importance.


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