Effect of a Chlorhexidine-containing Adhesive on Dentin Bond Strength Stability

2013 ◽  
Vol 38 (6) ◽  
pp. 609-617 ◽  
Author(s):  
C Sabatini
Keyword(s):  
Phosphoric Acid ◽  
Bond Strength ◽  
Storage Time ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gelatin Zymography ◽  
Control Group ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Self Etch

SUMMARY Purpose: The present study aimed to investigate a novel adhesive system containing 0.2% chlorhexidine digluconate (CHX) for its ability to improve the stability of the adhesive interface compared with the use of 2% CHX as a therapeutic primer. Furthermore, the study aimed to confirm the inhibitory properties of these CHX concentrations (0.2% and 2.0%) on dentin matrix metalloproteinase activity by gelatin zymography. Methods: Superficial dentin substrate for bonding was obtained from 120 non-carious human molars. A conventional adhesive Peak LC Bond and a CHX-containing adhesive Peak Universal Bond were used either in combination with 35% phosphoric acid (etch-and-rinse approach) or with self-etching primer (self-etch approach) for evaluation of the variables CHX treatment (2.0% therapeutic primer and 0.2% adhesive), adhesive approach (etch-and-rinse and self-etch), and storage time (24 hours and six months). A bonding jig was used to fabricate composite cylinders, which were stored for either 24 hours or six months, after which shear bond strength (SBS) was evaluated using a notched-edge testing device. A three-way analysis of variance and a Student t-test with a significance level of p<0.05 were used to analyze the data. Extracts from concentrated demineralized human dentin powder were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and incubated in the presence of 0.2% and 2.0% CHX. Results: No significant effect of CHX treatment, adhesive approach, storage time variables, or their interactions on mean SBS was demonstrated (p<0.05). No significant difference between the control and the CHX-treated groups was detected for either adhesive technique at 24 hours or six months (p<0.05). No significant variation in mean SBS was detected after six months of storage (p<0.05). Zymographic analysis revealed bands of enzymatic activity for the group demineralized with phosphoric acid and complete inhibition of gelatinolytic activity for the groups treated with 0.2% and 2.0% CHX. Conclusions: CHX demonstrated inhibition of dentin proteolytic activity. However, when CHX was incorporated into a commercially available adhesive or used as a therapeutic primer, no difference in bond strength was observed at baseline or after six months of storage relative to the control group without CHX.

scholarly journals Effect of adhesive mode and chlorhexidine on microtensile strength of universal bonding agent to sound and caries-affected dentins

2018 ◽  
Vol 12 (04) ◽  
pp. 553-558 ◽  
Author(s):  
Joyce Figueiredo Macedo de Lima ◽  
Danielle Wajngarten ◽  
Fariya Islam ◽  
Johanna Clifford ◽  
Ana Carolina Botta
Keyword(s):  
Phosphoric Acid ◽  
Bond Strength ◽  
Composite Resin ◽  
High Viscosity ◽  
Bonding Agent ◽  
Third Molars ◽  
Microtensile Bond Strength ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Self Etch

ABSTRACT Objective: The main objective of this study is to assess the effect of the adhesive mode and chlorhexidine (CHX) on the microtensile strength of a universal bonding agent to sound and caries-affected dentins. Materials and Methods: Six intact third molars and six decayed third molars were sectioned to obtain two middle dentin specimens per tooth. Prime and Bond Elect Universal (Dentsply) was applied to specimens in two different modes: Etch-and-rinse (ER, phosphoric acid for 15s) and self-etch (SE, no phosphoric acid). 2% CHX (Cavity Cleanser, Bisco) was applied to both dentins for 30 s. Specimens were randomly assigned into eight groups (n = 3) according to the treatments applied to sound and caries-affected dentins: SE; ER; SE + CHX; and ER + CHX. All specimens were restored with TPH Spectra High Viscosity (Dentsply) composite resin and sectioned to obtain specimens of 0.8 ± 0.1 mm2. The microtensile bond strength test was conducted at 1 mm/min. The data were statistically analyzed by ANOVA and multiple comparisons' test (α = 0.05). Results: There was no significant difference between sound dentin and caries-affected dentin (P = 0.132). The highest bond strength was obtained with the application of the ER (31.81MPa). SE + CHX promoted the lowest bond strength with no statistical difference to ER + CHX (P > 0.05). Conclusions: ER mode for the universal bonding agent yielded the highest bond strength to sound and caries-affected dentins. CHX reduced the bond strength to both dentins regardless the application of phosphoric acid. Clinical Significance: The universal bonding agent increased the bond strength to sound and caries-affected dentins when applied by the ER mode.

scholarly journals Effect of Proteolytic Agents on Microleakage of Etch and Rinse Adhesive Systems

2017 ◽  
Vol 16 ◽  
pp. 1-11
Author(s):  
Shahin Kasraei ◽  
Ebrahim Yarmohammadi ◽  
Maryam Farhadian ◽  
Mona Malek
Keyword(s):  
Phosphoric Acid ◽  
Nd:Yag Laser ◽  
Control Group ◽  
Single Bond ◽  
Adhesive Systems ◽  
Class V ◽  
Dentin Surface ◽  
Composite Restorations ◽  
Etch And Rinse ◽  
Significant Difference

Objective: this study aimed to assess the effect of treatment of phosphoric acid etched dentin surface with 5% bromelain enzyme and Nd:YAG laser prior to the use of etch and rinse adhesive systems on microleakage margins of class V composite restorations. Materials and Methods: sixty sound premolar teeth were selected. Standard class V cavities were prepared in the buccal and lingual surfaces of each teeth. Preparation in that way 1 mm of the cavity was above and 1 mm of it was below the cementoenamel junction. The teeth were then randomly divided into three groups including 20 teeth(40cavities  in each gorup). Two ethanol base etch and rinse system (Adper Single Bond and OptiBond Solo) was applied in each group. In the control group, cavities were etched with 37% phosphoric acid. Adper Single Bond was applied to 20 and OptiBond Solo was applied to the remaining 20 cavities and they were restored with Z250 composite (n=40). The same procedures were performed in bromelain and Nd:YAG laser groups with the difference that prior to the application of adhesive, aqueous 5% bromelain was applied in bromelain group while Nd:YAG laser was irradiated in laser group. All teeth were thermocycled and their apices were sealed with sticky wax. The tooth surfaces were coated with nail varnish except for 1 mm around the restoration margins, and the teeth were then immersed in fuchsine and sectioned by a diamond disc into mesial and distal halves. Marginal microleakage at both sides was determined and scored under a stereomicroscope at x40 magnification. Then data were analyzed using the Kruskal Wallis and Mann Whitney tests (α=0.05). Results: according the result of Mann Whitney test, Cervical margins showed significantly higher microleakage than the occlusal margins (P=0.000) but no significant difference was noted in microleakage of occlusal (P=0.362) or gingival (P=0.147) margins among the three groups by Kruskal Wallis test. Conclusion: in conclusion, application of proteolytic agents(5% bromelain solution and Nd:YAG laser)  on acid-etched dentin surface prior to the application of adhesive has no significant effect on marginal microleakage of class V composite restorations. 

scholarly journals Influence of Water Aging on Microtensile Bond Strength of a Flowable and a Packable Bulk-fill Resin Composites to Dentin

2020 ◽  
Vol 8 (D) ◽  
pp. 112-117
Author(s):  
Ayah Atif Selim ◽  
Ahmed Fawzy Abo Elezz ◽  
Rehab Khalil Safy
Keyword(s):  
Bond Strength ◽  
Storage Time ◽  
Resin Composite ◽  
Water Storage ◽  
Aging Effect ◽  
Mean Value ◽  
Control Group ◽  
Microtensile Bond Strength ◽  
Significant Difference ◽  
Influence Of Water

AIM: Investigation of the aging effect on the microtensile bond strength (μTBS) of bulk-fill resin composite (RC) versus a conventionally incrementally applied one. MATERIALS AND METHODS: A total number of 45 sound human impacted third molars extracted molars have been selected to prepare specimens for the μTBS test. Teeth were randomly divided into three groups (C) according to type of RC material which used for restoring the teeth. Where nanohybrid RC (Grandio®SO) was used as the control Group (C1), packable bulk-fill RC (X-tra fil®) was used for restoring teeth in C2 group and flowable bulk-fill RC (X-tra base®) was used for restoring teeth in C3 group. Each group was further subdivided into 3 subgroups (n = 5) according to the water storage time, where in subgroup 1; teeth were stored for 24 h, subgroup 2; teeth were stored for 3 months while for subgroup 3; and teeth were stored for 6 months. After water storage, teeth were sectioned for preparation of μTBS testing beams. Maximum tensile stresses were recorded in megapascal (MPa). RESULTS: After 24 h of water storage, the X-tra base® showed a higher statistically significant μTBS to dentin (33.82 ± 9.84 MPa) than did the other two types of RCs. After 3 months, the X-tra fil® showed the lowest mean value of μTBS (10.90 ± 5.66 MPa), meanwhile, after 6 months of water storage Grandio®SO showed the highest mean value of μTBS (15.85 ± 6.76 MPa). Regardless of the time the X-tra fil® showed the lowest mean of μTBS (15.07 ± 11.73 MPa), while there is no significant difference between the X-tra base® and Grandio®SO. Furthermore, the water aging adversely affects μTBS values which deceased gradually by time. CONCLUSION: The packable bulk-fill RC characterized by lower μTBS to dentin in comparison to the flowable bulk fill and the incrementally applied nanohybrid RCs. Furthermore, the μTBS of the three tested materials decreased gradually by aging.

scholarly journals Influence of Air Abrasion and Sonic Technique on Microtensile Bond Strength of One-Step Self-Etch Adhesive on Human Dentin

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Baraba Anja ◽  
Dukić Walter ◽  
Chieffi Nicoletta ◽  
Ferrari Marco ◽  
Sonja Pezelj Ribarić ◽  
...  
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Control Group ◽  
Air Abrasion ◽  
Microtensile Bond Strength ◽  
Dentin Surface ◽  
Significant Difference ◽  
Human Dentin ◽  
One Step ◽  
Self Etch

The purpose of thisin vitrostudy was to evaluate the microtensile bond strength of one-step self-etch adhesive to human dentin surface modified with air abrasion and sonic technique and to assess the morphological characteristics of the pretreated dentin surface. The occlusal enamel was removed to obtain a flat dentin surface for thirty-six human molar teeth. The teeth were randomly divided into three experimental groups (n= 12 per group), according to the pretreatment of the dentin: (1) control group, (2) air abrasion group, and (3) sonic preparation group. Microtensile bond strength test was performed on a universal testing machine. Two specimens from each experimental group were subjected to SEM examination. There was no statistically significant difference in bond strength between the three experimental groups (P> 0.05). Mean microtensile bond strength (MPa) values were 35.3 ± 12.8 for control group, 35.8 ± 13.5 for air abrasion group, and 37.7 ± 12.0 for sonic preparation group. The use of air abrasion and sonic preparation with one-step self-etch adhesive does not appear to enhance or impair microtensile bond strength in dentin.

Effect of Nd:YAG Laser Irradiation Pretreatment on the Long-Term Bond Strength of Etch-and-Rinse Adhesive to Dentin

2017 ◽  
Vol 42 (1) ◽  
pp. 62-72 ◽  
Author(s):  
J Gan ◽  
S Liu ◽  
L Zhou ◽  
Y Wang ◽  
J Guo ◽  
...  
Keyword(s):  
Bond Strength ◽  
Laser Irradiation ◽  
Nd:Yag Laser ◽  
Knoop Hardness ◽  
Control Group ◽  
In Vitro Test ◽  
Dentin Surface ◽  
Etch And Rinse ◽  
Significant Difference

SUMMARY Purpose: To investigate the effect of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation pretreatment on the long-term bond strength of an etch-and-rinse adhesive to dentin. Methods: Fifty molars were sectioned parallel to the occlusal plane and randomly divided into two groups (n=25 per group): control group (no treatment) and laser group (dentin surfaces were treated with Nd:YAG laser at a setting of 100 mJ/10 Hz). Afterward, resin was bonded to the dentin surface using a two-step etch-and-rinse adhesive (Adper SingleBond 2), and then 150 beams of each group were produced. Each group was divided into three subgroups (n=50 each group): 24 hours of water storage, thermocycling, and NaOCl storage. The microtensile bond strength (MTBS), failure modes, nanoleakage expression, and Masson's trichrome staining were evaluated. An additional 20 molars were sectioned to obtain 2-mm-thick flat dentin slices. These slices were randomly divided into control and laser-treated groups as mentioned previously. Then slices of each group were examined by scanning electron microscopy, attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), and the Knoop hardness test. Results: The results of ATR-FTIR and Masson's trichrome verified that laser irradiation partly removed collagen fibers from the dentin surface; however, no significant difference was found in the Knoop hardness (p>0.05). The XRD result showed similar crystalline structure regardless of laser pretreatment. There is no significant difference in short-term MTBS between control and laser-treated groups (p>0.05); however, long-term MTBS differed between the groups (p>0.05). Furthermore, the laser-treated group showed less silver deposition than the control group after aging (p<0.05). Conclusion: Pretreatment by Nd:YAG laser irradiation appeared to have a positive effect on the adhesive-dentin bonding in vitro test, and the bonding effectiveness could be preserved after aging.

scholarly journals Microshear Bond Strength of Different Adhesive Systems to Dentin

2019 ◽  
Author(s):  
Sara Valizadeh ◽  
Aida Moradi ◽  
Mansooreh Mirazei ◽  
Hooman Amiri ◽  
Mohammad Javad Kharazifard
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Single Bond ◽  
Adhesive Systems ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Microshear Bond Strength ◽  
Self Etch ◽  
Composite Cylinders

Objectives: The aim of this study was to compare the microshear bond strength (µSBS) of various adhesive systems to dentin. Materials and Methods: In this in vitro experimental study, 60 sound human third molars were divided into four groups. Dentin discs were prepared of middle-third dentin measuring 4 mm in diameter and 2 mm in thickness. Dentin surfaces were bonded with one of the four types of adhesives: (A) Single Bond (3M ESPE), Scotchbond Universal (3M ESPE) in etch and rinse (B) and self-etch (C) modes and (D) Clearfil SE Bond (Kuraray Noritake Dental). After the application of adhesive systems according to the manufacturers’ instructions, composite cylinders (Vit-l-escence) were bonded to dentin surfaces. The μSBS test was performed using a universal testing machine at a crosshead speed of 1 mm/min. Data were analyzed with one-way ANOVA and Tukey’s test (α=0.05). Results: The µSBS was the highest in self-etch Scotchbond Universal (15.8±6.08 MPa) followed by Clearfil SE Bond (15.24±4.6 MPa), etch and rinse Scotchbond Universal (11.68±4.07MPa) and Single Bond (11.24±3.74 MPa). A significant difference was only found between Single Bond and etch and rinse Scotchbond Universal groups (P=0.04). Conclusion: Based on the results of this study, application of Scotchbond Universal in self-etch mode provides a reliable bond to dentin.

scholarly journals Microshear Bond Strength of Different Adhesive Systems to Dentin

2019 ◽  
Author(s):  
Sara Valizadeh ◽  
Aida Moradi ◽  
Mansooreh Mirazei ◽  
Hooman Amiri ◽  
Mohammad Javad Kharazifard
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Single Bond ◽  
Adhesive Systems ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Microshear Bond Strength ◽  
Self Etch ◽  
Composite Cylinders

Objectives: The aim of this study was to compare the microshear bond strength (µSBS) of various adhesive systems to dentin. Materials and Methods: In this in vitro experimental study, 60 sound human third molars were divided into four groups. Dentin discs were prepared of middle-third dentin measuring 4 mm in diameter and 2 mm in thickness. Dentin surfaces were bonded with one of the four types of adhesives: (A) Single Bond (3M ESPE), Scotchbond Universal (3M ESPE) in etch and rinse (B) and self-etch (C) modes and (D) Clearfil SE Bond (Kuraray Noritake Dental). After the application of adhesive systems according to the manufacturers’ instructions, composite cylinders (Vit-l-escence) were bonded to dentin surfaces. The μSBS test was performed using a universal testing machine at a crosshead speed of 1 mm/min. Data were analyzed with one-way ANOVA and Tukey’s test (α=0.05). Results: The µSBS was the highest in self-etch Scotchbond Universal (15.8±6.08 MPa) followed by Clearfil SE Bond (15.24±4.6 MPa), etch and rinse Scotchbond Universal (11.68±4.07MPa) and Single Bond (11.24±3.74 MPa). A significant difference was only found between Single Bond and etch and rinse Scotchbond Universal groups (P=0.04). Conclusion: Based on the results of this study, application of Scotchbond Universal in self-etch mode provides a reliable bond to dentin.

scholarly journals The effects of different surface contaminators on the shear bond strength of a universal adhesive system to dentin: an experimental study

2021 ◽  
Vol 15 (2) ◽  
pp. 82-86
Author(s):  
Mahmoud Bahari ◽  
Siavash Savadi Oskoee ◽  
Mohammad Esmaeel Ebrahimi Chaharom ◽  
Nasim Molayi
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Adhesive System ◽  
Aluminium Chloride ◽  
Control Group ◽  
Universal Adhesive ◽  
Human Teeth ◽  
Etch And Rinse ◽  
Self Etch ◽  
Composite Samples

Background. Contamination of dentin surface is one of the common problems in restorative dentistry. The aim was to investigate the effects of different surface contaminators on the dentin shear bond strength (SBS) of universal adhesive system (UAS) applied in etch-and-rinse (ER) and self-etch (SE) strategies. Methods. One hundred forty-four maxillary anterior sound human teeth were divided into six groups based on the types of surface contaminators: no surface contaminator (control) and experimental groups contaminated with blood, saliva, aluminium chloride (ALC), ferric sulphate (FS), and caries disclosing agent (CDA). Then, each group was further subdivided into two, based on the application strategy of UAS (ER and SE). After applying the adhesive according to the manufacturer’s instructions, and bonding cylindrical composite samples, the SBS was measured. The data were analysed using two-way ANOVA, Tukey’s HSD test and t test (P<0.05). Results. The SBS in all contaminated groups, except for CDA, was significantly lower in both ER and SE strategies compared to control group (P<0.05). A comparison between the application strategies revealed that ER and SE were only significantly different in the FS contaminated group (P<0.05). Conclusion. All tested contaminators, except CDA, significantly decreased SBS of UAS in both ER and SE strategies.

Effect of Different Adhesive Systems Used for Immediate Dentin Sealing on Bond Strength of a Self-Adhesive Resin Cement to Dentin

2018 ◽  
Vol 43 (4) ◽  
pp. 391-397 ◽  
Author(s):  
RC Ferreira-Filho ◽  
C Ely ◽  
RC Amaral ◽  
JA Rodrigues ◽  
J-F Roulet ◽  
...  
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Water Storage ◽  
Resin Cement ◽  
Control Group ◽  
Adhesive Systems ◽  
Adhesive Resin ◽  
Etch And Rinse ◽  
Adhesive Resin Cement ◽  
Self Etch

SUMMARY Objective: The purpose of this study was to investigate the immediate and three-month water storage behavior of adhesives when used for immediate dentin sealing (IDS). Methods and Materials: Four adhesive systems were used to perform IDS: a one-step self-etch (Xeno V), a two-step self-etch (Clearfil SE Bond), a two-step etch-and-rinse (XP Bond), and a three-step etch-and-rinse (Optibond FL). For the control group, IDS was not performed. The self-adhesive resin cement RelyX Unicem was used for the luting procedures. After seven days of water storage, specimens (n=6) were sectioned into beams (n=5) with an approximately 1-mm2 cross-sectional area. Half of the specimens were tested in tension after seven days of water storage at 37°C, while the other half was stored for three months prior to testing in tension using a universal testing machine (1 mm/min). The failure pattern was determined using a stereomicroscope and scanning electron microscopy. Microtensile bond strength (μTBS) data were statistically analyzed by two-way analysis of variance and Tukey post hoc test (α=0.05). Results: After seven days, the control group presented the lowest μTBS but did not differ from XP Bond and Clearfil SE Bond. After three months, there was no μTBS difference between the IDS groups and the control. Conclusions: After seven days of water storage, the groups with IDS presented higher μTBS values than the control group, although XP Bond and Clearfil SE Bond did not present significant differences. However, after three months of storage in water, IDS groups did not differ significantly from control group, which did not receive IDS.

scholarly journals Influence of previous acid etching on interface morphology and bond strength of self-etching adhesive to cavosurface enamel

2012 ◽  
Vol 06 (01) ◽  
pp. 056-062 ◽  
Author(s):  
Adriano Fonseca Lima ◽  
Vinícius Brito da Silva ◽  
Giulliana Panfiglio Soares ◽  
Giselle Maria Marchi ◽  
Flávio Henrique Baggio Aguiar ◽  
...  
Keyword(s):  
Phosphoric Acid ◽  
Bond Strength ◽  
Adhesive System ◽  
The Self ◽  
The Other ◽  
Acid Etching ◽  
Control Group ◽  
System Control ◽  
Etch And Rinse ◽  
Group 3

ABSTRACTObjectives: The aim of this study was to evaluate the (1) bond strength of a etch-and-rinse and self-etching adhesive systems to cavosurface enamel, (2) influence of the previous acid etching with phosphoric acid 35% to the self-etching adhesive application on bond strength values, and (3) analysis of the cavosurface enamel morphology submitted to different types of conditioning, with the use of a scanning electronic microscope (SEM).Methods: Twenty four human third molars were sectioned on mesio-distal direction, resulting in two slices. The specimens were ground flat with 600-grit aluminum oxide papers, and were randomly divided into three groups: Group 1 (etch-and-rinse adhesive system (control group)), Group 2 (selfetching adhesive), and Group 3 (self-etching adhesive with previous 35% phosphoric acid-etching for 15 s). Four cylinders (0.75 mm of diameter, 1 mm height) were confectioned prior to the microshear test. Four samples for each group were prepared according the cavosurface enamel treatment and were analyzed in an SEM.Results: Group 3 had the highest values on bond strength to cavosurface enamel compared to the other two groups, which presented statistically similar values. The performance of acid etching before the application of the self-etching adhesive results in an etching pattern that is different than the other groups, favoring the adhesion to the cavosurface enamel.Conclusions: Acid etching increases the bond strength values of the self-etching adhesive to cavosurface enamel, promoting a conditioning pattern that favors the adhesion to this substrate. (Eur J Dent 2012;6:56-62)

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