680-P: Residual ß-Cell Function in Long-Term Type 1 Diabetes Is Associated with a Fivefold Greater Mobilization of Endothelial Progenitor Cells after Exercise

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 680-P
Author(s):  
GUY S. TAYLOR ◽  
KIERAN SMITH ◽  
JADINE SCRAGG ◽  
AYAT BASHIR ◽  
ANNELIESE FLATT ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Cell Function ◽  
Endothelial Progenitor ◽  
Term Type

Glucose variability inversely associates with endothelial progenitor cells in type 1 diabetes

Endocrine ◽  
2014 ◽  
Vol 48 (1) ◽  
pp. 342-345 ◽  
Author(s):  
Maria Ida Maiorino ◽  
Elisabetta Della Volpe ◽  
Laura Olita ◽  
Giuseppe Bellastella ◽  
Dario Giugliano ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Glucose Variability ◽  
Endothelial Progenitor

scholarly journals Beta cell function during rapamycin monotherapy in long-term type 1 diabetes

Diabetologia ◽  
2010 ◽  
Vol 54 (2) ◽  
pp. 433-439 ◽  
Author(s):  
L. Piemonti ◽  
P. Maffi ◽  
L. Monti ◽  
V. Lampasona ◽  
G. Perseghin ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Beta Cell ◽  
Beta Cell Function ◽  
Cell Function ◽  
Term Type

scholarly journals Relationship between circulating endothelial progenitor cells and endothelial dysfunction in children with type 1 diabetes: a novel paradigm of early atherosclerosis in high-risk young patients

2013 ◽  
Vol 168 (2) ◽  
pp. 153-161 ◽  
Author(s):  
Barbara Głowińska-Olszewska ◽  
Marcin Moniuszko ◽  
Andrzej Hryniewicz ◽  
Marta Jeznach ◽  
Małgorzata Rusak ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Vascular Function ◽  
Diabetic Patients ◽  
Endothelial Progenitor ◽  
Circulating Endothelial Progenitor Cells ◽  
Increased Risk ◽  
Diabetic Children

ObjectiveThe low number of circulating endothelial progenitor cells (EPCs) has emerged as a biomarker of cardiovascular (CV) risk in adults. Data regarding EPCs in paediatric populations with CV risk factors are limited. The aim of the study was to estimate the EPC number and its relationship with vascular function and structure in children with type 1 diabetes mellitus (T1DM).Design and methodsWe performed a comparative analysis of 52 children with T1DM (mean age 14.5 years; diabetes duration, 6.0 years; HbA1c level, 8.5%) and 36 healthy age- and gender-matched control children. EPCs were identified and analysed by flow cytometry with the use of MABs directed against CD34, CD144 (VE-cadherin) and CD309 (VEGFR-2). sICAM-1, hsCRP, thrombomodulin and adiponectin levels were also assessed. We evaluated vascular function (flow-mediated dilation (FMD)) and structure (carotid intima–media thickness (IMT)) ultrasonographically.ResultsFrequencies of CD34+ cells were similar in both groups (P=0.30). In contrast, frequencies of CD34+VE-cadherin+ cells were significantly higher in diabetic children compared with the healthy group (P=0.003). Similarly, diabetic patients tended to present with higher frequencies of CD34+VEGFR+ cells (P=0.06). FMD was lower (6.9 vs 10.5%, P=0.002) and IMT was higher (0.50 vs 0.44 mm, P=0.0006) in diabetic children. We demonstrated a significant relationship between CD34+VEGFR-2+ cells and BMI (r=0.3, P=0.014), HDL (r=−0.27, P=0.04), sICAM-1 (r=0.47, P=0.023) and FMD (r=−0.45, P<0.001). Similarly, frequencies of CD34+VE-cadherin+ cells were significantly correlated with BMI (r=0.32, P=0.02) and FMD (r=−0.31, P=0.03).ConclusionsWe demonstrated here that increased frequencies of EPCs observed in diabetic children are negatively correlated with endothelial function. Further studies are warranted to assess whether this phenomenon might result from effective mobilisation of EPCs in order to repair damaged endothelium in children at increased risk for atherosclerosis.

scholarly journals Impact of glycemic variability on the levels of endothelial progenitor cells in patients with type 1 diabetes

2017 ◽  
Vol 9 (2) ◽  
pp. 113-120 ◽  
Author(s):  
Yuiko Inaba ◽  
Chiharu Tsutsumi ◽  
Fumitaka Haseda ◽  
Reiko Fujisawa ◽  
Shinobu Mitsui ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Glycemic Variability ◽  
Endothelial Progenitor

scholarly journals Endothelial Progenitor Cells Are Related to Glycemic Control in Children With Type 1 Diabetes Over Time

Diabetes Care ◽  
2013 ◽  
Vol 36 (6) ◽  
pp. 1647-1653 ◽  
Author(s):  
T. Hortenhuber ◽  
B. Rami-Mehar ◽  
M. Satler ◽  
K. Nagl ◽  
C. Hobaus ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Glycemic Control ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor ◽  
Over Time

scholarly journals MiR-126, IL-7, CXCR1/2 receptors, inflammation and circulating endothelial progenitor cells: The study on targets for treatment pathways in a model of subclinical cardiovascular disease (type 1 diabetes mellitus)

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
David J. Coulson ◽  
Sherin Bakhashab ◽  
Jevi Septyani Latief ◽  
Jolanta U. Weaver
Keyword(s):  
Cardiovascular Disease ◽  
Type 1 Diabetes ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Inflammatory Cytokines ◽  
Endothelial Progenitor ◽  
Circulating Endothelial Progenitor Cells ◽  
Inflammatory State ◽  
Pro Inflammatory Cytokines

Abstract Background Type 1 diabetes (T1DM) is associated with premature cardiovascular disease (CVD) and a pro-inflammatory state whilst the proangiogenic miR-126-3p/-5p may play a role in CVD. Animal studies established miR-126 to be pro-angiogenic. We hypothesised miR-126-3p/-5p are reduced in T1DM whilst pro-inflammatory cytokines are increased. Methods 29 well controlled, T1DM patients without CVD and 20 healthy controls (HCs) were studied. MiR-126-3p/-5p were assayed in plasma and peripheral blood mononuclear cells (PBMCs) whilst Chemokine C-X-C Receptor 1/2 (CXCR1/2) mRNA in PBMCs by real-time quantitative PCR. Cytokines were assayed by the Mesoscale Discovery. Ingenuity Pathway Analysis (IPA) was used to predict target genes, cellular functions and pathological states regulated by miR-126-3p/-5p. IPA generated both direct and indirect causations between different targets and analysed whether these effects would be inhibitory or stimulatory based on the published evidence. Results T1DM patients had a relatively good diabetic control (HbA1c = 7.4 ± 0.7% or 57.3 ± 7.6 mmol/mol). Homeostatic cytokine IL-7, pro-inflammatory cytokines IL-8 and TNF-α, and vascular endothelial growth factor-C (VEGF-C) were increased in T1DM, versus HCs; p = 0.008, p = 0.003, p = 0.041 and p = 0.013 respectively. MiR-126-5p was significantly upregulated in PBMCs in T1DM versus HCs; p = 0.01, but not in plasma. MiR-126-3p was unchanged. CXCR1/2 were elevated in T1DM versus HCs; p = 0.009 and p < 0.001 respectively. MiR-126-5p was positively correlated with CXCR1/2, and with HbA1c whilst negatively correlated with circulating endothelial progenitor cells (CD34+CD133+CD45dim) and fibronectin adhesion assay in a combined group of T1DM patients and HCs; p = 0.028 p = 0.049 p = 0.035 p = 0.047 and p = 0.004 respectively. IPA predicted miR-126-5p to be anti-inflammatory through the inhibition of chemokine C–C motif ligand 27, chymotrypsin-like elastase 2A and IL-7, whilst miR-126-3p had no direct anti-inflammatory effect. Simultaneously IPA predicted IL-7 as the most upstream cytokine target. Conclusions T1DM without apparent CVD or diabetic complications is an inflammatory state characterised not only by raised pro-inflammatory cytokines but also by increased receptor CXCR1/2 and miR-126-5p. MiR-126-5p upregulation may represent a compensatory response. Pro-miR-126-5p therapies or anti-IL-7 therapies may be a new option to reduce both inflammation and CVD risk in T1DM. Further research is required in a large prospective study in patients with T1DM.

scholarly journals Upregulated anti-angiogenic miR-424-5p in type 1 diabetes (model of subclinical cardiovascular disease) correlates with endothelial progenitor cells, CXCR1/2 and other parameters of vascular health

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Alice Tamara ◽  
David J. Coulson ◽  
Jevi Septyani Latief ◽  
Sherin Bakhashab ◽  
Jolanta U. Weaver
Keyword(s):  
Cardiovascular Disease ◽  
Type 1 Diabetes ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Adhesion Assay ◽  
Vascular Health ◽  
Endothelial Progenitor ◽  
Tnf Α ◽  
Subclinical Cvd

Abstract Background In spite of clinical progress, cardiovascular disease (CVD) remains the predominant cause of mortality worldwide. Overexpression studies in animals have proven miR-424-5p to have anti-angiogenic properties. As type 1 diabetes mellitus (T1DM) without CVD displays endothelial dysfunction and reduced circulating endothelial progenitor cells (cEPCs), it offers a model of subclinical CVD. Therefore, we explored miR-424-5p, cytokines and vascular health in T1DM. Methods Twenty-nine well-controlled T1DM patients with no CVD and 20-matched controls were studied. Cytokines IL8, TNF-α, IL7, VEGF-C, cEPCs/CD45dimCD34+CD133+ cells and ex-vivo proangiogenic cells (PACs)/fibronectin adhesion assay (FAA) were measured. MiR-424-5p in plasma and peripheral blood mononuclear cells (PBMC) along with mRNAs in PBMC was evaluated. Results We found an elevation of IL7 (p = 0.008), IL8 (p = 0.003), TNF-α (p = 0.041), VEGF-C (p = 0.013), upregulation of mRNA CXCR1 (p = 0.009), CXCR2 (p < 0.001) and reduction of cEPCs (p < 0.001), PACs (p < 0.001) and FAA (p = 0.017) in T1DM. MiR-424-5p was upregulated in T1DM in PBMC (p < 0.001). MiR-424-5p was negatively correlated with cEPCs (p = 0.006), PACs (p = 0.005) and FAA (p < 0.001) and positively with HbA1c (p < 0.001), IL7 (p = 0.008), IL8 (p = 0.017), VEGF-C (p = 0.007), CXCR1 (p = 0.02) and CXCR2 (p = 0.001). ROC curve analyses showed (1) miR-424-5p to be a biomarker for T1DM (p < 0.001) and (2) significant upregulation of miR-424-5p, defining subclinical CVD, occurred at HbA1c of 46.5 mmol/mol (p = 0.002). Conclusion We validated animal research on anti-angiogenic properties of miR-424-5p in T1DM. MiR-424-5p may be a biomarker for onset of subclinical CVD at HbA1c of 46.5 mmol/mol (pre-diabetes). Thus, miR-424-5p has potential use for CVD monitoring whilst anti-miR-424-5p-based therapies may be used to reduce CVD morbidity/mortality in T1DM.

scholarly journals Residual β cell function in long-term type 1 diabetes associates with reduced incidence of hypoglycemia

2021 ◽  
Vol 131 (3) ◽  
Author(s):  
Rose A. Gubitosi-Klug ◽  
Barbara H. Braffett ◽  
Susan Hitt ◽  
Valerie Arends ◽  
Diane Uschner ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Cell Function ◽  
Β Cell ◽  
Β Cell Function ◽  
Term Type
Sign in / Sign up

Export Citation Format

Share Document