Vascular expression of permeability-resistant occludin mutant preserves visual function in diabetes

Diabetes ◽  
2021 ◽  
pp. db201220
Author(s):  
Andreia Goncalves ◽  
Alyssa Dreffs ◽  
Cheng-mao Lin ◽  
Sarah Sheskey ◽  
Natalie Hudson ◽  
...  
Keyword(s):  
Visual Function ◽  
Vascular Expression

scholarly journals Vascular Expression of Permeability-Resistant Occludin Mutant Preserves Visual Function in Diabetes

2021 ◽  
Author(s):  
Andreia Goncalves ◽  
Alyssa Dreffs ◽  
Cheng-mao Lin ◽  
Sarah Sheskey ◽  
Natalie Hudson ◽  
...  
Keyword(s):  
Tight Junction ◽  
Visual Function ◽  
Vision Loss ◽  
Neurovascular Unit ◽  
Barrier Properties ◽  
Dependent Manner ◽  
Specific Expression ◽  
Edema Formation ◽  
Junction Protein ◽  
Vascular Expression

Diabetic retinopathy is one of the leading causes of vision loss and blindness. Extensive pre-clinical and clinical evidence exists for both vascular and neuronal pathology. However, the relationship of these changes in the neurovascular unit and impact on vision remains to be determined. Here, we investigate the role of tight junction protein occludin phosphorylation at S490 in modulating barrier properties and its impact on visual function. Conditional vascular expression of the phosphorylation resistant Ser490 to Ala (S490A) form of occludin preserved tight junction organization and reduced VEGF-induced permeability and edema formation after intra-ocular injection. In the retinas of streptozotocin-induced diabetic mice, endothelial specific expression of the S490A form of occludin completely prevented diabetes-induced permeability to labeled dextran and inhibited leukostasis. Importantly, vascular-specific expression of the occludin mutant completely blocked the diabetes-induced decrease in visual acuity and contrast sensitivity. Together, these results reveal that occludin acts to regulate barrier properties downstream of VEGF in a phosphorylation dependent manner and that loss of inner blood-retinal barrier (iBRB) integrity induced by diabetes contributes to vision loss.

scholarly journals Vascular Expression of Permeability-Resistant Occludin Mutant Preserves Visual Function in Diabetes

2021 ◽  
Author(s):  
Andreia Goncalves ◽  
Alyssa Dreffs ◽  
Cheng-mao Lin ◽  
Sarah Sheskey ◽  
Natalie Hudson ◽  
...  
Keyword(s):  
Tight Junction ◽  
Visual Function ◽  
Vision Loss ◽  
Neurovascular Unit ◽  
Barrier Properties ◽  
Dependent Manner ◽  
Specific Expression ◽  
Edema Formation ◽  
Junction Protein ◽  
Vascular Expression

Diabetic retinopathy is one of the leading causes of vision loss and blindness. Extensive pre-clinical and clinical evidence exists for both vascular and neuronal pathology. However, the relationship of these changes in the neurovascular unit and impact on vision remains to be determined. Here, we investigate the role of tight junction protein occludin phosphorylation at S490 in modulating barrier properties and its impact on visual function. Conditional vascular expression of the phosphorylation resistant Ser490 to Ala (S490A) form of occludin preserved tight junction organization and reduced VEGF-induced permeability and edema formation after intra-ocular injection. In the retinas of streptozotocin-induced diabetic mice, endothelial specific expression of the S490A form of occludin completely prevented diabetes-induced permeability to labeled dextran and inhibited leukostasis. Importantly, vascular-specific expression of the occludin mutant completely blocked the diabetes-induced decrease in visual acuity and contrast sensitivity. Together, these results reveal that occludin acts to regulate barrier properties downstream of VEGF in a phosphorylation dependent manner and that loss of inner blood-retinal barrier (iBRB) integrity induced by diabetes contributes to vision loss.

Cognitive competence at the onset of West syndrome: correlation with EEG patterns and visual function

2005 ◽  
Vol 47 (11) ◽  
pp. 760 ◽  
Author(s):  
Teresa Randò ◽  
Giovanni Baranello ◽  
Daniela Ricci ◽  
Andrea Guzzetta ◽  
Francesca Tinelli ◽  
...  
Keyword(s):  
Visual Function ◽  
West Syndrome ◽  
Cognitive Competence

Prevention of Visual Function in the Direct Surgery of Paraclinoid Aneurysms

2014 ◽  
Vol 75 (S 02) ◽  
Author(s):  
Yutaka Mine ◽  
H. Ishimori ◽  
H. Murakami ◽  
T. Horikoshi ◽  
M. Ishihara
Keyword(s):  
Visual Function ◽  
Paraclinoid Aneurysms

Diagnosis of visual function in patients with loss of central vision

2019 ◽  
pp. 225-228
Author(s):  
A.P. Ermolaev ◽  
◽  
V.P. Erichev ◽  
A.A. Antonov ◽  
Y.V. Levitsky ◽  
...  
Keyword(s):  
Visual Function ◽  
Central Vision
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