scholarly journals H3K4 Trimethylation is Required for Postnatal Pancreatic Endocrine Cell Functional Maturation

Diabetes ◽  
2021 ◽  
pp. db201214
Author(s):  
Stephanie A. Campbell ◽  
Jocelyn Bégin ◽  
Cassandra L. McDonald ◽  
Ben Vanderkruk ◽  
Tabea L. Stephan ◽  
...  
Keyword(s):  
Endocrine Cell ◽  
Pancreatic Endocrine Cell ◽  
H3k4 Trimethylation ◽  
Functional Maturation

scholarly journals H3K4 Trimethylation is Required for Postnatal Pancreatic Endocrine Cell Functional Maturation

2021 ◽  
Author(s):  
Stephanie A. Campbell ◽  
Jocelyn Bégin ◽  
Cassandra L. McDonald ◽  
Ben Vanderkruk ◽  
Tabea L. Stephan ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Cell Differentiation ◽  
B Cells ◽  
Endocrine Cell ◽  
Insulin Response ◽  
H3k4 Trimethylation ◽  
Fasting Glycemia ◽  
Functional Maturation ◽  
Glucose Stimulated Insulin Secretion ◽  
Endocrine Progenitors

During pancreas development, endocrine progenitors differentiate into the islet-cell subtypes, which undergo further functional maturation in postnatal islet development. In islet b-cells, genes involved in glucose-stimulated insulin secretion are activated and glucose exposure increases the insulin response as b-cells mature. Here, we investigated the role of H3K4 trimethylation in endocrine cell differentiation and functional maturation by disrupting TrxG complex histone methyltransferase activity in mouse endocrine progenitors. In the embryo, genetic inactivation of TrxG component <i>Dpy30</i> in NEUROG3+ cells did not affect the number of endocrine progenitors or endocrine cell differentiation. H3K4 trimethylation was progressively lost in postnatal islets and the mice displayed elevated non-fasting and fasting glycemia, as well as impaired glucose tolerance by postnatal day 24. Although postnatal endocrine cell proportions were equivalent to controls, islet RNA-sequencing revealed a downregulation of genes involved in glucose-stimulated insulin secretion and an upregulation of immature b-cell genes. Comparison of histone modification enrichment profiles in NEUROG3+ endocrine progenitors and mature islets suggested that genes downregulated by loss of H3K4 trimethylation more frequently acquire active histone modifications during maturation. Taken together, these findings suggest that H3K4 trimethylation is required for the activation of genes involved in the functional maturation of pancreatic islet endocrine cells.

scholarly journals H3K4 trimethylation is required for postnatal pancreatic endocrine cell functional maturation

2020 ◽  
Author(s):  
Stephanie A. Campbell ◽  
Jocelyn Bégin ◽  
Cassandra L. McDonald ◽  
Ben Vanderkruk ◽  
Tabea L. Stephan ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Cell Differentiation ◽  
Endocrine Cell ◽  
Insulin Response ◽  
Β Cells ◽  
H3k4 Trimethylation ◽  
Fasting Glycemia ◽  
Functional Maturation ◽  
Glucose Stimulated Insulin Secretion ◽  
Endocrine Progenitors

SummaryDuring pancreas development, endocrine progenitors differentiate into the islet-cell subtypes, which undergo further functional maturation in postnatal islet development. In islet β-cells, genes involved in glucose-stimulated insulin secretion are activated and glucose exposure increases the insulin response as β-cells mature. Here, we investigated the role of H3K4 trimethylation in endocrine cell differentiation and functional maturation by disrupting TrxG complex histone methyltransferase activity in mouse endocrine progenitors. In the embryo, genetic inactivation of TrxG component Dpy30 in NEUROG3+ cells did not affect the number of endocrine progenitors or endocrine cell differentiation. H3K4 trimethylation was progressively lost in postnatal islets and the mice displayed elevated random and fasting glycemia, as well as impaired glucose tolerance by postnatal day 24. Although postnatal endocrine cell proportions were equivalent to controls, islet RNA-sequencing revealed a downregulation of genes involved in glucose-stimulated insulin secretion and an upregulation of immature β-cell genes. Comparison of histone modification enrichment profiles in NEUROG3+ endocrine progenitors and mature islets suggested that genes downregulated by loss of H3K4 trimethylation more frequently acquire active histone modifications during maturation. Taken together, these findings suggest that H3K4 trimethylation is required for the activation of genes involved in the functional maturation of pancreatic islet endocrine cells.

scholarly journals Dynamics of chromatin marks and the role of JMJD3 during pancreatic endocrine cell fate commitment

Development ◽  
2018 ◽  
Vol 145 (6) ◽  
pp. dev163162 ◽  
Author(s):  
Xin-Xin Yu ◽  
Wei-Lin Qiu ◽  
Liu Yang ◽  
Lin-Chen Li ◽  
Yu-Wei Zhang ◽  
...  
Keyword(s):  
Cell Fate ◽  
Endocrine Cell ◽  
Pancreatic Endocrine Cell

scholarly journals Specifying pancreatic endocrine cell fates

2006 ◽  
Vol 123 (7) ◽  
pp. 501-512 ◽  
Author(s):  
Patrick Collombat ◽  
Jacob Hecksher-Sørensen ◽  
Palle Serup ◽  
Ahmed Mansouri
Keyword(s):  
Endocrine Cell ◽  
Cell Fates ◽  
Pancreatic Endocrine Cell

Pancreatic Endocrine Cell Fractions in Erythroblastosis Fetalis

Diabetes ◽  
1983 ◽  
Vol 32 (4) ◽  
pp. 313-315 ◽  
Author(s):  
R. D. G. Milner ◽  
F. Dinsdale ◽  
P. K. Wirdnam ◽  
F. A. van Assche
Keyword(s):  
Endocrine Cell ◽  
Pancreatic Endocrine Cell ◽  
Cell Fractions

scholarly journals Effect of the Extracellular Matrix on Pancreatic Endocrine Cell Function and its Biocompatibility in Dogs

2001 ◽  
Vol 10 (4-5) ◽  
pp. 493-498 ◽  
Author(s):  
Kazuya Edamura ◽  
Hisako Ohgawara ◽  
Koko Nasu ◽  
Yukiko Iwami ◽  
Ayako Sato ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endocrine Cell ◽  
Cell Function ◽  
Pancreatic Endocrine Cell

scholarly journals Mouse Pancreatic Endocrine Cell Transcriptome Defined in the Embryonic Ngn3-Null Mouse

Diabetes ◽  
2008 ◽  
Vol 57 (10) ◽  
pp. 2755-2761 ◽  
Author(s):  
K. Juhl ◽  
S. A. Sarkar ◽  
R. Wong ◽  
J. Jensen ◽  
J. C. Hutton
Keyword(s):  
Endocrine Cell ◽  
Null Mouse ◽  
Pancreatic Endocrine Cell ◽  
Cell Transcriptome

Localization of APP, a postulated new hormone, to a pancreatic endocrine cell type

1974 ◽  
Vol 42 (4) ◽  
pp. 377-382 ◽  
Author(s):  
L. -I. Larsson ◽  
F. Sundler ◽  
R. H�kanson ◽  
H. G. Pollock ◽  
J. R. Kimmel
Keyword(s):  
Endocrine Cell ◽  
Cell Type ◽  
Pancreatic Endocrine Cell ◽  
Endocrine Cell Type
Sign in / Sign up

Export Citation Format

Share Document