scholarly journals Endothelial SHIP2 Suppresses Nox2 NADPH Oxidase–Dependent Vascular Oxidative Stress, Endothelial Dysfunction, and Systemic Insulin Resistance

Diabetes ◽  
2017 ◽  
Vol 66 (11) ◽  
pp. 2808-2821 ◽  
Author(s):  
Nicole T. Watt ◽  
Matthew C. Gage ◽  
Peysh A. Patel ◽  
Hema Viswambharan ◽  
Piruthivi Sukumar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Endothelial Dysfunction ◽  
Nadph Oxidase ◽  
Systemic Insulin Resistance ◽  
Vascular Oxidative Stress

Abstract 357: Hepatic ERK2 Suppresses Endoplasmic Reticulum Stress, Leading to Protection from Oxidative Stress and Endothelial Dysfunction

2013 ◽  
Vol 33 (suppl_1) ◽  
Author(s):  
Takehiko Kujiraoka ◽  
Yasushi Satoh ◽  
Makoto Ayaori ◽  
Yasunaga Shiraishi ◽  
Yuko Arai-Nakaya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Endoplasmic Reticulum ◽  
Fatty Acid ◽  
Endothelial Dysfunction ◽  
Er Stress ◽  
Vascular Complications ◽  
Metabolic Remodeling ◽  
And Oxidative Stress

Background Insulin signaling comprises 2 major cascades, the IRS/PI3K/Akt and Ras/Raf/MEK/ERK pathways. Many studies on the tissue-specific effects of the former pathway had been conducted, however, the role of the latter cascade in tissue-specific insulin resistance had not been investigated. High glucose/fatty acid toxicity, inflammation and oxidative stress, all of which are associated with insulin resistance, can activate ERK. Liver plays a central role of metabolism and hepatosteatosis (HST) is associated with vascular diseases. The aim of this study is to elucidate the role of hepatic ERK2 in HST, metabolic remodeling and endothelial dysfunction. Methods Serum biomarkers of vascular complications in human were compared between subjects with and without HST diagnosed by echography for regular medical checkup. Next, we created liver-specific ERK2 knockout mice (LE2KO) and fed them with a high-fat/high-sucrose diet (HFHSD) for 20 weeks. The histological analysis, the expression of hepatic sarco/endoplasmic reticulum (ER) Ca 2+ -ATPase 2 (SERCA2) and glucose-tolerance/insulin-sensitivity (GT/IS) were tested. Vascular superoxide production and endothelial function were evaluated with dihydroethidium staining and isometric tension measurement of aorta. Results The presence of HST significantly increased HOMA-IR, an indicator of insulin resistance or atherosclerotic index in human. HFHSD-fed LE2KO revealed a marked exacerbation in HST and metabolic remodeling represented by the impairment of GT/IS, elevated serum free fatty acid and hyperhomocysteinemia without changes in body weight, blood pressure and serum cholesterol/triglyceride levels. In the HFHSD-fed LE2KO, mRNA and protein expressions of hepatic SERCA2 were significantly decreased, which resulted in hepatic ER stress. Induction of vascular superoxide production and remarkable endothelial dysfunction were also observed in them. Conclusions Hepatic ERK2 revealed the suppression of hepatic ER stress and HST in vivo , which resulted in protection from vascular oxidative stress and endothelial dysfunction. HST with hepatic ER stress can be a prominent risk of vascular complications by metabolic remodeling and oxidative stress in obese-related diseases.

Daily Treatment with Sildenafil Reverses Endothelial Dysfunction and Oxidative Stress in an Animal Model of Insulin Resistance

2008 ◽  
Vol 53 (6) ◽  
pp. 1272-1281 ◽  
Author(s):  
Delphine Behr-Roussel ◽  
Alexandra Oudot ◽  
Stéphanie Caisey ◽  
Olivier L.E. Coz ◽  
Diane Gorny ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Animal Model ◽  
Endothelial Dysfunction ◽  
Daily Treatment ◽  
And Oxidative Stress

Oxidative stress and endothelial dysfunction

2007 ◽  
Vol 27 (01) ◽  
pp. 5-12 ◽  
Author(s):  
G. Muller ◽  
C. Goettsch ◽  
H. Morawietz
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
Vascular Function ◽  
Vessel Wall ◽  
Clinical Implications ◽  
Oxygen Species ◽  
Vascular Oxidative Stress ◽  
Arteries And Veins ◽  
Different Sources

SummaryThis review focuses on the role of vascular oxidative stress in the development and progression of endothelial dysfunction. We discuss different sources of oxidative stress in the vessel wall, oxidative stress and coagulation, the role of oxidative stress and vascular function in arteries and veins, the flow-dependent regulation of reactive oxygen species, the putative impact of oxidative stress on atherosclerosis, the interaction of angiotensin II, oxidative stress and endothelial dysfunction, and clinical implications.

scholarly journals Differential contribution of Nox1, Nox2 and Nox4 to kidney vascular oxidative stress and endothelial dysfunction in obesity

Redox Biology ◽  
2020 ◽  
Vol 28 ◽  
pp. 101330 ◽  
Author(s):  
Mercedes Muñoz ◽  
Maria Elvira López-Oliva ◽  
Claudia Rodríguez ◽  
María Pilar Martínez ◽  
Javier Sáenz-Medina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
Vascular Oxidative Stress ◽  
Differential Contribution

The PPARγ ligand, rosiglitazone, reduces vascular oxidative stress and NADPH oxidase expression in diabetic mice

2007 ◽  
Vol 46 (6) ◽  
pp. 456-462 ◽  
Author(s):  
Jinah Hwang ◽  
Dean J. Kleinhenz ◽  
Heidi L. Rupnow ◽  
Adam G. Campbell ◽  
Peter M. Thulé ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nadph Oxidase ◽  
Diabetic Mice ◽  
Vascular Oxidative Stress

scholarly journals 232 Endothelial ship2 knockout causes nox2 nadph oxidase-dependent oxidative stress and endothelial dysfunction

Heart ◽  
2017 ◽  
Vol 103 (Suppl 5) ◽  
pp. A149.1-A149
Author(s):  
Peysh Patel ◽  
Nicole Watt ◽  
Matthew Gage ◽  
Nadira Yuldasheva ◽  
Hema Viswambharan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
Nadph Oxidase

scholarly journals Contribution of monoamine oxidases to vascular oxidative stress in patients with end-stage renal disease requiring hemodialysis

2017 ◽  
Vol 95 (11) ◽  
pp. 1383-1388 ◽  
Author(s):  
Diana Uțu ◽  
Stelian Pantea ◽  
Oana M. Duicu ◽  
Danina M. Muntean ◽  
Adrian Sturza
Keyword(s):  
Oxidative Stress ◽  
Endothelial Dysfunction ◽  
Renal Disease ◽  
Vascular Access ◽  
End Stage Renal Disease ◽  
Mao Inhibitors ◽  
Monoamine Oxidases ◽  
Stage Renal Disease ◽  
End Stage ◽  
Vascular Oxidative Stress

Arteriovenous fistula (AVF) is the “lifeline” for patients with end-stage renal disease (ESRD) undergoing hemodialysis. AVF maturation failure is a poorly understood process, one of the contributors being endothelial dysfunction due to oxidative stress. Monoamine oxidases (MAOs) A and B were recently identified as novel sources of vascular oxidative stress. The aim of the present study was to assess the contribution of MAOs to the endothelial dysfunction in patients with ESDR with indication of hemodialysis. Fragments of brachial artery collaterals were harvested from ESRD patients during the surgical procedure aimed at creating the vascular access in the cubital fossa. The effect of increasing concentrations (10, 30, 100 μmol/L) of the irreversible MAO-A inhibitor, clorgyline, and MAO-B inhibitor, selegiline, on endothelial-dependent relaxation (EDR) in response to cumulative doses of acetylcholine was studied in isolated phenylephrine-preconstricted vascular rings. Hydrogen peroxide (H2O2) production was assessed using ferrous oxidation xylenol orange assay. We showed that incubation of brachial rings with MAO inhibitors significantly improved EDR and attenuated H2O2 generation in patients with ESRD. MAO-related oxidative stress might contribute to the primary dysfunction/non-maturation of the AVF and MAO inhibitors could improve maturation and long-term patency of the vascular access in dialysis patients.

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