scholarly journals  -Cell-Specific Protein Kinase A Activation Enhances the Efficiency of Glucose Control by Increasing Acute-Phase Insulin Secretion

Diabetes ◽  
2013 ◽  
Vol 62 (5) ◽  
pp. 1527-1536 ◽  
Author(s):  
K. A. Kaihara ◽  
L. M. Dickson ◽  
D. A. Jacobson ◽  
N. Tamarina ◽  
M. W. Roe ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Acute Phase ◽  
Glucose Control ◽  
Specific Protein ◽  
Kinase A ◽  
Specific Protein Kinase

scholarly journals Matrix-Specific Protein Kinase A Signaling Regulates p21-Activated Kinase Activation by Flow in Endothelial Cells

2010 ◽  
Vol 106 (8) ◽  
pp. 1394-1403 ◽  
Author(s):  
Steven Daniel Funk ◽  
Arif Yurdagul ◽  
Jonette M. Green ◽  
Krishna A. Jhaveri ◽  
Martin Alexander Schwartz ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Specific Protein ◽  
Kinase Activation ◽  
P21 Activated Kinase ◽  
Kinase A ◽  
Specific Protein Kinase

Regulation of NHE3 by nitric oxide in Caco-2 cells

2002 ◽  
Vol 283 (3) ◽  
pp. G747-G756 ◽  
Author(s):  
Ravinder K. Gill ◽  
Seema Saksena ◽  
Irfan Ali Syed ◽  
Sangeeta Tyagi ◽  
Waddah A. Alrefai ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Guanylate Cyclase ◽  
Soluble Guanylate Cyclase ◽  
Signal Transduction Pathway ◽  
Specific Protein ◽  
Protein Kinase G ◽  
Kinase A ◽  
Specific Protein Kinase

The effect of nitric oxide (NO) on Na+/H+ exchange (NHE) activity was investigated utilizing Caco-2 cells as an experimental model. Incubation of Caco-2 cells with 10−3 M S-nitroso- N-acetylpenicillamine (SNAP), a conventional donor of NO, for 20 min resulted in a ∼45% dose-dependent decrease in NHE activity, as determined by assay of ethylisopropylamiloride-sensitive 22Na uptake. A similar decrease in NHE activity was observed utilizing another NO-specific donor, sodium nitroprusside. SNAP-mediated inhibition of NHE activity was not secondary to a loss of cell viability. NHE3 activity was significantly reduced by SNAP ( P < 0.05), whereas NHE2 activity was essentially unaltered. The effects of SNAP were mediated by the cGMP-dependent signal transduction pathway as follows: 1) LY-83583 and 1 H-(1,2,4)oxadiazolo(4,3- a)quinoxalin-1-one (ODQ), specific inhibitors of soluble guanylate cyclase, blocked the inhibitory effect of SNAP on NHE; 2) 8-bromo-cGMP mimicked the effects of SNAP on NHE activity; 3) the SNAP-induced decrease in NHE activity was counteracted by a specific protein kinase G inhibitor, KT-5823 (1 μM); 4) chelerythrine chloride (2 μM) or calphostin C (200 nM), specific protein kinase C inhibitors, did not affect inhibition of NHE activity by SNAP; 5) there was no cross activation by the protein kinase A-dependent pathway, as the inhibitory effects of SNAP were not blocked by Rp-cAMPS (25 μM), a specific protein kinase A inhibitor. These data provide novel evidence that NO inhibits NHE3 activity via activation of soluble guanylate cyclase, resulting in an increase in intracellular cGMP levels and activation of protein kinase G.

scholarly journals Isolation and Molecular Characterization of AKAP110, a Novel, Sperm-Specific Protein Kinase A-Anchoring Protein

1999 ◽  
Vol 13 (5) ◽  
pp. 705-717 ◽  
Author(s):  
Srinivasan Vijayaraghavan ◽  
Greg A. Liberty ◽  
Jag Mohan ◽  
Virginia P. Winfrey ◽  
Gary E. Olson ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Molecular Characterization ◽  
Specific Protein ◽  
Anchoring Protein ◽  
Kinase A ◽  
Specific Protein Kinase

scholarly journals Sperm-specific protein kinase A catalytic subunit C 2 orchestrates cAMP signaling for male fertility

2004 ◽  
Vol 101 (37) ◽  
pp. 13483-13488 ◽  
Author(s):  
M. A. Nolan ◽  
D. F. Babcock ◽  
G. Wennemuth ◽  
W. Brown ◽  
K. A. Burton ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Male Fertility ◽  
Specific Protein ◽  
Catalytic Subunit ◽  
Camp Signaling ◽  
Subunit C ◽  
Kinase A ◽  
Specific Protein Kinase
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