scholarly journals Involvement of Per-Arnt-Sim Kinase and Extracellular-Regulated Kinases-1/2 in Palmitate Inhibition of Insulin Gene Expression in Pancreatic  -Cells

Diabetes ◽  
2009 ◽  
Vol 58 (9) ◽  
pp. 2048-2058 ◽  
Author(s):  
G. Fontes ◽  
M. Semache ◽  
D. K. Hagman ◽  
C. Tremblay ◽  
R. Shah ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Gene ◽  
Pancreatic Cells ◽  
Insulin Gene Expression

scholarly journals Negative regulation of human insulin gene expression by the 5′-flanking region in non-pancreatic cells

FEBS Letters ◽  
1989 ◽  
Vol 247 (1) ◽  
pp. 41-45 ◽  
Author(s):  
Jun Takeda ◽  
Shunsuke Ishii ◽  
Yutaka Seino ◽  
Fumio Imamoto ◽  
Hiroo Imura
Keyword(s):  
Gene Expression ◽  
Human Insulin ◽  
Negative Regulation ◽  
Insulin Gene ◽  
Pancreatic Cells ◽  
Human Insulin Gene ◽  
Insulin Gene Expression ◽  
Flanking Region

Extinction of insulin gene expression in hybrids between beta cells and fibroblasts is accompanied by loss of the putative beta-cell-specific transcription factor IEF1

1991 ◽  
Vol 11 (3) ◽  
pp. 1547-1552
Author(s):  
D Leshkowitz ◽  
M D Walker
Keyword(s):  
Gene Expression ◽  
Dna Binding ◽  
Binding Activity ◽  
Insulin Gene ◽  
Hybrid Cells ◽  
Dna Binding Activity ◽  
Insulin Producing Cells ◽  
Insulin Gene Expression ◽  
Insulinoma Cells

Insulin-producing cells and fibroblasts were fused to produce hybrid lines. In hybrids derived from both hamster and rat insulinoma cells, no insulin mRNA could be detected in any of seven lines examined by Northern (RNA) analysis despite the presence in each line of the insulin genes of both parental cells. Hybrid cells were transfected with recombinant chloramphenicol acetyltransferase plasmids containing defined segments of the rat insulin I gene 5' flank. We observed no transcriptional activity of the intact insulin enhancer or of IEB2, a critical cis-acting element of the insulin enhancer. IEB2 has previously been shown to interact in vitro with IEF1, a DNA-binding activity observed selectively in insulin-producing cells. Hybrid cells showed no detectable IEF1 activity. Furthermore, the insulin enhancer was unable to reduce transcription directed by the Moloney sarcoma virus enhancer in a double-enhancer construct. Thus, extinction of insulin gene expression in the hybrids apparently does not operate through a direct action of repressors on the insulin enhancer; rather, extinction is accompanied by, and may be caused by, reduced DNA-binding activity of the putative transcriptional activator IEF1.

scholarly journals Palmitate Inhibition of Insulin Gene Expression Is Mediated at the Transcriptional Level via Ceramide Synthesis

2003 ◽  
Vol 278 (32) ◽  
pp. 30015-30021 ◽  
Author(s):  
Cynthia L. Kelpe ◽  
Patrick C. Moore ◽  
Susan D. Parazzoli ◽  
Barton Wicksteed ◽  
Christopher J. Rhodes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Gene ◽  
Transcriptional Level ◽  
Ceramide Synthesis ◽  
Insulin Gene Expression

Regulation of Insulin-Gene Expression

1987 ◽  
Vol 317 (17) ◽  
pp. 1067-1076 ◽  
Author(s):  
Jeffrey S. Flier ◽  
Lisa H. Underhill ◽  
Richard F. Selden ◽  
Marek J. Skośkiewicz ◽  
Paul S. Russell ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insulin Gene ◽  
Insulin Gene Expression
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