scholarly journals Complete genome sequencing of Red Sea Bream Iridovirus (RSIV)

2002 ◽  
Vol 68 (sup2) ◽  
pp. 1113-1115 ◽  
Author(s):  
JUN KURITA ◽  
KAZUHIRO NAKAJIMA ◽  
IKUO HIRONO ◽  
TAKASHI AOKI
Keyword(s):  
Genome Sequencing ◽  
Red Sea ◽  
Complete Genome ◽  
Sea Bream ◽  
Red Sea Bream ◽  
Complete Genome Sequencing

Isolation and characterisation of an ISKNV-genotype megalocytivirus from imported angelfish Pterophyllum scalare

2020 ◽  
Vol 140 ◽  
pp. 129-141
Author(s):  
Y Kawato ◽  
PG Mohr ◽  
MStJ Crane ◽  
LM Williams ◽  
MJ Neave ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Red Sea ◽  
Sea Bream ◽  
Whole Genome ◽  
Red Sea Bream ◽  
Capsid Protein Gene ◽  
Infected Cells ◽  
Clade 1 ◽  
Pterophyllum Scalare

Using cultures of the SKF-9 cell line, megalocytivirus AFIV-16 was isolated from imported angelfish Pterophyllum scalare held in quarantine at the Australian border. The cytopathic effect caused by isolate AFIV-16 presented as cell rounding and enlargement, but complete destruction of the infected cell cultures did not occur. The infected cells demonstrated immunocytochemical reactivity with monoclonal antibody M10, which is used for diagnosis of OIE-listed red sea bream iridoviral disease. Using electron microscopy, the virus particles, consisting of hexagonal nucleocapsids, were observed in the cytoplasm of SKF-9 cells. The replication of AFIV-16 in cultured SKF-9 cells was significantly greater at 28°C incubation than at 22 and 25°C incubation, whereas no difference in growth characteristics was observed for red sea bream iridovirus (RSIV) isolate KagYT-96 across this temperature range. Whole genome sequencing demonstrated that AFIV-16 has a 99.96% similarity to infectious spleen and kidney necrosis virus (ISKNV), the type species in the genus Megalocytivirus. AFIV-16 was classified into ISKNV genotype Clade 1 by phylogenetic analysis of the major capsid protein gene nucleotide sequence. This is the first report of whole genome sequencing of an ISKNV genotype megalocytivirus isolated from ornamental fish.

scholarly journals Whole genome sequencing reveals the impact of recent artificial selection on red sea bream reared in fish farms

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Bo-Hye Nam ◽  
DongAhn Yoo ◽  
Young-Ok Kim ◽  
Jung Youn Park ◽  
Younhee Shin ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Red Sea ◽  
Artificial Selection ◽  
Sea Bream ◽  
Whole Genome ◽  
Red Sea Bream ◽  
Fish Farms ◽  
The Impact

scholarly journals Complete Genome Sequences and Pathogenicity Analysis of Two Red Sea Bream Iridoviruses Isolated from Cultured Fish in Korea

Fishes ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 82
Author(s):  
Min-A Jeong ◽  
Ye-Jin Jeong ◽  
Kwang-Il Kim
Keyword(s):  
Red Sea ◽  
Complete Genome ◽  
Survival Rates ◽  
Open Reading Frames ◽  
Sea Bream ◽  
Red Sea Bream ◽  
Genome Sequences ◽  
Oplegnathus Fasciatus ◽  
Cultured Fish ◽  
Japanese Seabass

In Korea, red sea bream iridovirus (RSIV), especially subtype II, has been the main causative agent of red sea bream iridoviral disease since the 1990s. Herein, we report two Korean RSIV isolates with different subtypes based on the major capsid protein and adenosine triphosphatase genes: 17SbTy (RSIV mixed subtype I/II) from Japanese seabass (Lateolabrax japonicus) and 17RbGs (RSIV subtype II) from rock bream (Oplegnathus fasciatus). The complete genome sequences of 17SbTy and 17RbGs were 112,360 and 112,235 bp long, respectively (115 and 114 open reading frames [ORFs], respectively). Based on nucleotide sequence homology with sequences of representative RSIVs, 69 of 115 ORFs of 17SbTy were most closely related to subtype II (98.48–100% identity), and 46 were closely related to subtype I (98.77–100% identity). In comparison with RSIVs, 17SbTy and 17RbGs carried two insertion/deletion mutations (ORFs 014R and 102R on the basis of 17SbTy) in regions encoding functional proteins (a DNA-binding protein and a myristoylated membrane protein). Notably, survival rates differed significantly between 17SbTy-infected and 17RbGs-infected rock breams, indicating that the genomic characteristics and/or adaptations to their respective original hosts might influence pathogenicity. Thus, this study provides complete genome sequences and insights into the pathogenicity of two newly identified RSIV isolates classified as a mixed subtype I/II and subtype II.

scholarly journals Author Correction: Whole genome sequencing reveals the impact of recent artificial selection on red sea bream reared in fish farms

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Bo-Hye Nam ◽  
DongAhn Yoo ◽  
Young-Ok Kim ◽  
Jung Youn Park ◽  
Younhee Shin ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Red Sea ◽  
Artificial Selection ◽  
Sea Bream ◽  
Whole Genome ◽  
Red Sea Bream ◽  
Fish Farms ◽  
The Impact

Purification, characterization, and molecular cloning of group I phospholipases A2 from the gills of the red sea bream, Pagrus major

Lipids ◽  
2000 ◽  
Vol 35 (12) ◽  
pp. 1359-1371 ◽  
Author(s):  
Noriaki Iijima ◽  
Satoshi Uchiyama ◽  
Yukichi Fujikawa ◽  
Muneharu Esaka
Keyword(s):  
Molecular Cloning ◽  
Red Sea ◽  
Sea Bream ◽  
Red Sea Bream ◽  
Pagrus Major ◽  
Phospholipases A2 ◽  
Group I
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