Yolk proteins in the giant freshwater prawn, Macrobrachium rosenbergii: Determination of amino acid sequence and site of production

2002 ◽  
Vol 68 (sup1) ◽  
pp. 700-703
Author(s):  
MARCY WILDER ◽  
KATSUMI AIDA
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Yolk Proteins ◽  
Giant Freshwater Prawn

Determination of amino acid sequence and site of mRNA expression of four vitellins in the giant freshwater prawn,Macrobrachium rosenbergii

2000 ◽  
Vol 287 (6) ◽  
pp. 413-422 ◽  
Author(s):  
Wei-Jun Yang ◽  
Tsuyoshi Ohira ◽  
Naoaki Tsutsui ◽  
Thanumalayaperumal Subramoniam ◽  
Do Thi Thanh Huong ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Mrna Expression ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn

scholarly journals Amino acid compounds released by the giant freshwater prawn Macrobrachium rosenbergii during ecdysis: a factor attracting cannibalistic behaviour

2020 ◽  
Vol 21 (10) ◽  
pp. 823-834
Author(s):  
Abu Seman Juneta-Nor ◽  
Noordiyana Mat Noordin ◽  
Mohamad Nor Azra ◽  
Hong-yu Ma ◽  
Norainy Mohd Husin ◽  
...  
Keyword(s):  
Amino Acid ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn ◽  
Cannibalistic Behaviour

scholarly journals Osmoregulation of glutamine synthetase from Giant freshwater prawn (Macrobrachium rosenbergii) under osmotic stress

2019 ◽  
Author(s):  
Zhijie Lu ◽  
Zhendong Qin ◽  
V Sarath Babu ◽  
Chengkai Ye ◽  
Guomao Su ◽  
...  
Keyword(s):  
Amino Acid ◽  
Glutamine Synthetase ◽  
Osmotic Stress ◽  
Mrna Expression ◽  
Macrobrachium Rosenbergii ◽  
Amino Acid Sequences ◽  
Post Treatment ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn ◽  
Key Enzyme

AbstractGlutamine synthetase is a key enzyme that catalyzes the biosynthesis of glutamine (Gln) from glutamate and ammonia. Gln a vital amino acid acts as a precursor for protein synthesis and also assist in ammonia repressor and a key osmoregulators in aquatics. Here, we report the cloning and characterization of the GS gene from Macrobrachium rosenbergii (Mr-GS). The complete nucleotide and deduced amino acid sequences were determined that phylogenetically shared highest identity with other crustaceans. GS mRNA was differentially expressed in 6 different tissues, with high to low order as muscle > gills > heart > stomach > brain > haemolymph. Mr-GS expression and the glutamine concentrations were analyzed in the gills and muscle tissues of prawn under hyper/hypo-osmotic stress conditions. Under hyper-osmotic stress, the mRNA expression of Mr-GS was significantly increased in both gills and muscle at 3, 6 and 12 h post-treatment with 2.54, 4.21 and 10.83 folds, and 11.66, 17.97 and 45.92 folds, respectively. Protein analysis by western blot (WB) and Immunohistochemistry (IHC) further confirmed the Mr-GS expression was increased at 12 h post treatment. On the other hand, under hypo-osmotic stress, the mRNA expression of Mr-GS was also significantly increased in both gills and muscle at 3, 6 and 12 h post treatment with 1.63, 3.30 and 3.52 folds, and 4.06, 42.99 and 26.69 folds, respectively. Furthermore, under hyperosmotic stress, Gln concentration was increased in both gills and muscle at 6 and 12 h post treatment with 1.83, 2.02 folds, and 1.41, 1.29 folds, respectively. While, under hypo-osmotic stress, Gln concentration was increased in both gills and muscle at 3, 6 and 12 h post treatment with 3.99, 3.40, 2.59 folds, and 1.72, 1.83, 1.80 folds, respectively. Taken together, these results suggest that Mr-GS might play a key role in osmoregulation in M. rosenbergii.

Polypeptides Folding: Rules for the Calculation of the Backbone Dihedral Angles φ starting from the Amino Acid Sequence

2020 ◽  
Author(s):  
Michele Larocca
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Theoretical Approach ◽  
Polypeptide Chain ◽  
Hydrophobic Effect ◽  
Side Chain ◽  
Dihedral Angles ◽  
Mechanical Forces ◽  
Specific Chemical

<p>Protein folding is strictly related to the determination of the backbone dihedral angles and depends on the information contained in the amino acid sequence as well as on the hydrophobic effect. To date, the type of information embedded in the amino acid sequence has not yet been revealed. The present study deals with these problematics and aims to furnish a possible explanation of the information contained in the amino acid sequence, showing and reporting rules to calculate the backbone dihedral angles φ. The study is based on the development of mechanical forces once specific chemical interactions are established among the side chain of the residues in a polypeptide chain. It aims to furnish a theoretical approach to predict backbone dihedral angles which, in the future, may be applied to computational developments focused on the prediction of polypeptide structures.</p>

scholarly journals Characterization of prostanoid pathway and the control of its activity by the eyestalk optic ganglion in the female giant freshwater prawn, Macrobrachium rosenbergii

Heliyon ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. e05898
Author(s):  
Tipsuda Thongbuakaew ◽  
Chanudporn Sumpownon ◽  
Attakorn Engsusophon ◽  
Napamanee Kornthong ◽  
Charoonroj Chotwiwatthanakun ◽  
...  
Keyword(s):  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn ◽  
Optic Ganglion

Microcystin-LR disturbs testicular development of giant freshwater prawn Macrobrachium rosenbergii

Chemosphere ◽  
2019 ◽  
Vol 222 ◽  
pp. 584-592 ◽  
Author(s):  
Yingying Zhang ◽  
Hang Zhuang ◽  
Hui Yang ◽  
Wen Xue ◽  
Liufu Wang ◽  
...  
Keyword(s):  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Testicular Development ◽  
Giant Freshwater Prawn

scholarly journals Multi-biomarker assessment in the giant freshwater prawn Macrobrachium rosenbergii after deltamethrin exposure

2021 ◽  
Vol 214 ◽  
pp. 112067
Author(s):  
Qun Jiang ◽  
Ziyan Jiang ◽  
Shiqi Ao ◽  
Xiaojian Gao ◽  
Xinhai Zhu ◽  
...  
Keyword(s):  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
Giant Freshwater Prawn
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