Effect of Freezing and Thawing on Post-mortem Biochemical Changes in Scallop Adductor Muscle

1995 ◽  
Vol 61 (4) ◽  
pp. 691-695 ◽  
Author(s):  
Kaoru Kawashima ◽  
Hideaki Yamanaka
1994 ◽  
Vol 60 (4) ◽  
pp. 461-465 ◽  
Author(s):  
Kaoru Kawashima ◽  
Hideaki Yamanaka

1989 ◽  
Vol 68 (2) ◽  
pp. 249-257 ◽  
Author(s):  
C.E. LYON ◽  
C.E. DAVIS ◽  
J.A. DICKENS ◽  
C.M. PAPA ◽  
J.O. REAGAN

1968 ◽  
Vol 109 (2) ◽  
pp. 197-202 ◽  
Author(s):  
R. K. Scopes ◽  
R. P. Newbold

1. Ox sternomandibularis muscle was ‘slow-frozen’ by placing it in air at −22° or ‘fast-frozen’ by immersion in liquid air or acetone–solid carbon dioxide. In all cases muscles were frozen pre-rigor. Changes in length, pH and the concentrations of Pi, creatine phosphate, hexose monophosphate (glucose 1-phosphate+glucose 6-phosphate+fructose 6-phosphate), fructose diphosphate (fructose 1,6-diphosphate+½ triose phosphate), lactate, ATP, ADP, AMP and NAD+ during freezing and during subsequent thawing were determined. In addition some measurements were made of the changes in α-glycerophosphate, 3-phosphoglycerate, 2-phosphoglycerate, phosphoenolpyruvate and pyruvate concentrations during slow freezing. 2. Appreciable shortening and marked changes in chemical composition took place during slow freezing but not during fast freezing. 3. During slow freezing the hexose monophosphate concentration fell and fructose 1,6-diphosphate and triose phosphate increased substantially. Increases also took place in 3-phosphoglycerate, 2-phosphoglycerate and phosphoenolpyruvate, but not in pyruvate. 4. On thawing, most of the chemical changes were similar to those in unfrozen muscle post mortem, but took place much more rapidly; loss of NAD+ was particularly rapid. Fast-frozen muscle metabolized at a faster rate on thawing than did slow-frozen muscle. 5. The overall changes in length during freezing and thawing were about the same in slow-frozen as in fast-frozen muscle.


PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10134 ◽  
Author(s):  
He Wang ◽  
Mario Schädel ◽  
Benjamin Sames ◽  
David J. Horne

Burmese Cretaceous amber (∼99 Ma, Myanmar) is famous for the preservation of a wide range of fauna and flora, including representatives of marine, freshwater and terrestrial groups. Here, we report on three ostracod specimens, that came visible as syninclusions to an aquatic isopod. The three specimens represent three different taxa, that were found preserved in a single piece of amber. One of the described specimens was studied using µCT scanning data. On the basis of general carapace morphology we assign all three to the group Podocopida, and (tentatively) its ingroup Cypridocopina. A lack of visibility of more particular diagnostic features such as adductor muscle scars and details of the marginal zone precludes a further identification, but we discuss possible affinities with either the marine-brackish group Pontocypridoidea or the non-marine group Cypridoidea. The taphonomy indicates that the studied ostracods had been subject to limited (if any) post-mortem transport, which could be consistent with marginal marine environments.


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