scholarly journals Rapid diagnosis of periodontitis based on enzymatic activity derived from periodontopathic bacteria. Evaluation of the efficacy of periodontal pocket curettage by SK-013.

1991 ◽  
Vol 33 (1) ◽  
pp. 164-171
Author(s):  
Noriko SUGITA ◽  
Etsuko SATOH ◽  
Hideaki TAI ◽  
Tetsuo KOBAYASHI ◽  
Hiromasa YOSHIE ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Rapid Diagnosis ◽  
Periodontal Pocket ◽  
Periodontopathic Bacteria

scholarly journals Enzymatic activity of bacteria from periodontal pocket with advancing periodontitis.

1988 ◽  
Vol 30 (2) ◽  
pp. 515-525
Author(s):  
Jun-ichi INOUE ◽  
Hisanori FUKUSHIMA ◽  
Yukari TOYOSHIMA ◽  
Takashi SASAKI ◽  
Kohji YAMAMOTO ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Periodontal Pocket

scholarly journals Relationship between oral status and prevalence of periodontopathic bacteria on the tongues of elderly individuals

2010 ◽  
Vol 59 (11) ◽  
pp. 1354-1359 ◽  
Author(s):  
Mitsuo Kishi ◽  
Yuko Ohara-Nemoto ◽  
Masahiro Takahashi ◽  
Kayo Kishi ◽  
Shigenobu Kimura ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Community Dwelling ◽  
Periodontal Pocket ◽  
Treponema Denticola ◽  
Prevotella Intermedia ◽  
Systemic Diseases ◽  
Tongue Coating ◽  
Elderly Individuals ◽  
Periodontopathic Bacteria ◽  
Increased Risk

Colonization of periodontopathic bacteria is associated with increased risk of systemic diseases. However, few studies have investigated the relationships between oral status factors and health-related quality of life (HR-QOL) and the prevalence of such bacteria in elderly individuals. This study investigated the prevalence of Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola and Tannerella forsythia in 165 community-dwelling functionally independent 85-year-old Japanese individuals (93 dentate, 72 edentulous) and the relationship to oral status, including oral malodour and HR-QOL. All four of the studied periodontopathic bacteria were found more frequently in tongue coating samples from dentate than edentulous subjects, and the prevalence of Porphyromonas gingivalis, Prevotella intermedia and Treponema denticola was significantly related to the number of teeth with a periodontal pocket depth ≥4 mm. These results suggest the existence of a stable circulation of periodontopathic bacteria between the gingival sulcus and tongue coating over time with teeth. In addition, the presence of teeth with a deep pocket and colonization of Treponema denticola were positively related to the level of CH3SH, whilst the number of present teeth contributed positively to HR-QOL, especially with regard to mental health. In conclusion, as the dentate state can retain colonization of periodontopathic pathogens in the oral cavity, both periodontal treatment and tongue care are important for maintaining a healthy oral status in the elderly, and possibly result in avoidance of risk for tooth loss and decline in HR-QOL, as well as protecting from systemic diseases.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

A Method for the Colorimetric Determination of β-Glucuronidase in Urine, Serum, Tissue; Assay of Enzymatic Activity in Health and Disease

1959 ◽  
Vol 36 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Julius A. Goldbarg ◽  
Esteban P. Pineda ◽  
Benjamin M. Banks ◽  
Alexander M. Rutenburg
Keyword(s):  
Enzymatic Activity ◽  
Colorimetric Determination ◽  
Health And Disease ◽  
Urine Serum
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