scholarly journals Molecular Insights of Natural Rubber Biosynthesis-An Approach from Prenyltransferase Gene Analysis

2003 ◽  
Vol 76 (12) ◽  
pp. 446-452
Author(s):  
Seiji TAKAHASHI ◽  
Tanetoshi KOYAMA
Keyword(s):  
Natural Rubber ◽  
Gene Analysis ◽  
Rubber Biosynthesis ◽  
Natural Rubber Biosynthesis

scholarly journals A Lettuce (Lactuca sativa) Homolog of Human Nogo-B Receptor Interacts withcis-Prenyltransferase and Is Necessary for Natural Rubber Biosynthesis

2014 ◽  
Vol 290 (4) ◽  
pp. 1898-1914 ◽  
Author(s):  
Yang Qu ◽  
Romit Chakrabarty ◽  
Hue T. Tran ◽  
Eun-Joo G. Kwon ◽  
Moonhyuk Kwon ◽  
...  
Keyword(s):  
Natural Rubber ◽  
Lactuca Sativa ◽  
Rubber Biosynthesis ◽  
Natural Rubber Biosynthesis

Molecular Genetic Analysis of Natural Rubber Biosynthesis

2021 ◽  
Vol 68 (1) ◽  
pp. 31-45
Author(s):  
A. Yu. Amerik ◽  
Yu. Ts. Martirosyan ◽  
L. Yu. Martirosyan ◽  
V. M. Goldberg ◽  
K. R. Uteulin ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Natural Rubber ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Rubber Biosynthesis ◽  
Natural Rubber Biosynthesis

scholarly journals Identification and Characterization of Glycoproteins and Their Responsive Patterns upon Ethylene Stimulation in the Rubber Latex

2020 ◽  
Vol 21 (15) ◽  
pp. 5282 ◽  
Author(s):  
Li Yu ◽  
Boxuan Yuan ◽  
Lingling Wang ◽  
Yong Sun ◽  
Guohua Ding ◽  
...  
Keyword(s):  
Natural Rubber ◽  
Mevalonate Pathway ◽  
Interaction Analysis ◽  
Rubber Tree ◽  
Protein Isoforms ◽  
Wild Type Plant ◽  
Rubber Latex ◽  
Rubber Biosynthesis ◽  
In The Wild ◽  
Natural Rubber Biosynthesis

Natural rubber is an important industrial material, which is obtained from the only commercially cultivated rubber tree, Hevea brasiliensis. In rubber latex production, ethylene has been extensively used as a stimulant. Recent research showed that post-translational modifications (PTMs) of latex proteins, such as phosphorylation, glycosylation and ubiquitination, are crucial in natural rubber biosynthesis. In this study, comparative proteomics was performed to identify the glycosylated proteins in rubber latex treated with ethylene for different days. Combined with Pro-Q Glycoprotein gel staining and mass spectrometry techniques, we provided the first visual profiling of glycoproteomics of rubber latex and finally identified 144 glycosylated protein species, including 65 differentially accumulated proteins (DAPs) after treating with ethylene for three and/or five days. Gene Ontology (GO) functional annotation showed that these ethylene-responsive glycoproteins are mainly involved in cell parts, membrane components and metabolism. Pathway analysis demonstrated that these glycosylated rubber latex proteins are mainly involved in carbohydrate metabolism, energy metabolism, degradation function and cellular processes in rubber latex metabolism. Protein–protein interaction analysis revealed that these DAPs are mainly centered on acetyl-CoA acetyltransferase and hydroxymethylglutaryl-CoA synthase (HMGS) in the mevalonate pathway for natural rubber biosynthesis. In our glycoproteomics, three protein isoforms of HMGS2 were identified from rubber latex, and only one HMGS2 isoform was sharply increased in rubber latex by ethylene treatment for five days. Furthermore, the HbHMGS2 gene was over-expressed in a model rubber-producing grass Taraxacum Kok-saghyz and rubber content in the roots of transgenic rubber grass was significantly increased over that in the wild type plant, indicating HMGS2 is the key component for natural rubber production.

Molecular Mechanisms of Natural Rubber Biosynthesis

2020 ◽  
Vol 89 (1) ◽  
pp. 821-851 ◽  
Author(s):  
Satoshi Yamashita ◽  
Seiji Takahashi
Keyword(s):  
Natural Rubber ◽  
Biosynthetic Pathway ◽  
Molecular Mechanisms ◽  
Rubber Tree ◽  
Lipid Monolayer ◽  
Rubber Biosynthesis ◽  
Key Enzyme ◽  
Membrane Bound ◽  
Natural Rubber Biosynthesis ◽  
Natural Hydrocarbon

Natural rubber (NR), principally comprising cis-1,4-polyisoprene, is an industrially important natural hydrocarbon polymer because of its unique physical properties, which render it suitable for manufacturing items such as tires. Presently, industrial NR production depends solely on latex obtained from the Pará rubber tree, Hevea brasiliensis. In latex, NR is enclosed in rubber particles, which are specialized organelles comprising a hydrophobic NR core surrounded by a lipid monolayer and membrane-bound proteins. The similarity of the basic carbon skeleton structure between NR and dolichols and polyprenols, which are found in most organisms, suggests that the NR biosynthetic pathway is related to the polyisoprenoid biosynthetic pathway and that rubber transferase, which is the key enzyme in NR biosynthesis, belongs to the cis-prenyltransferase family. Here, we review recent progress in the elucidation of molecular mechanisms underlying NR biosynthesis through the identification of the enzymes that are responsible for the formation of the NR backbone structure.

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