scholarly journals BACTERIAL FORMATION OF L-GLUTAMIC ACID FROM ACETIC ACID IN THE GROWING CULTURE MEDIUM

1961 ◽  
Vol 7 (1) ◽  
pp. 18-29 ◽  
Author(s):  
TOSHINAO TSUNODA ◽  
ISAMU SHIIO ◽  
KOJI MITSUGI
Keyword(s):  
Acetic Acid ◽  
Glutamic Acid ◽  
Culture Medium

scholarly journals BACTERIAL FORMATION OF L-GLUTAMIC ACID FROM ACETIC ACID IN THE GROWING CULTURE MEDIUM

1961 ◽  
Vol 7 (1) ◽  
pp. 30-40 ◽  
Author(s):  
TOSHINAO TSUNODA ◽  
ISAMU SHIIO ◽  
KOJI MITSUGI
Keyword(s):  
Acetic Acid ◽  
Glutamic Acid ◽  
Culture Medium

Thermal adaptation of acetic acid bacteria for practical high-temperature vinegar fermentation

2021 ◽  
Vol 85 (5) ◽  
pp. 1243-1251
Author(s):  
Nami Matsumoto ◽  
Naoki Osumi ◽  
Minenosuke Matsutani ◽  
Theerisara Phathanathavorn ◽  
Naoya Kataoka ◽  
...  
Keyword(s):  
Acetic Acid ◽  
High Temperature ◽  
Experimental Evolution ◽  
Culture Medium ◽  
Thermal Adaptation ◽  
Acetic Acid Bacteria ◽  
Acid Fermentation ◽  
Acetic Acid Production ◽  
Fermentation System ◽  
Fermentor Culture

ABSTRACT Thermotolerant microorganisms are useful for high-temperature fermentation. Several thermally adapted strains were previously obtained from Acetobacter pasteurianus in a nutrient-rich culture medium, while these adapted strains could not grow well at high temperature in the nutrient-poor practical culture medium, “rice moromi.” In this study, A. pasteurianus K-1034 originally capable of performing acetic acid fermentation in rice moromi was thermally adapted by experimental evolution using a “pseudo” rice moromi culture. The adapted strains thus obtained were confirmed to grow well in such the nutrient-poor media in flask or jar-fermentor culture up to 40 or 39 °C; the mutation sites of the strains were also determined. The high-temperature fermentation ability was also shown to be comparable with a low-nutrient adapted strain previously obtained. Using the practical fermentation system, “Acetofermenter,” acetic acid production was compared in the moromi culture; the results showed that the adapted strains efficiently perform practical vinegar production under high-temperature conditions.

scholarly journals A method for the separation of peptides and α-amino acids

1968 ◽  
Vol 107 (3) ◽  
pp. 335-340 ◽  
Author(s):  
D. K. J. Tommel ◽  
J. F. G. Vliegenthart ◽  
T. J. Penders ◽  
J F Arens
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Acetate Buffer ◽  
Acid Fraction ◽  
Peptide Fraction ◽  
Acidic Peptide ◽  
Peptide Fractions ◽  
Acetate Form

1. Peptides and α-amino acids, occurring in mixtures from various sources, can be separated into one fraction containing the amino acids and several peptide fractions. This is achieved by chelation of the mixture with Cu2+ ions and subsequent chromatography of these chelates over the acetate form of diethylaminoethylcellulose or triethylaminoethylcellulose. 2. The amino acid fraction is obtained by elution with 0·01m-collidine–acetate buffer, pH8·0. 3. Peptide fractions are eluted with 0·01m-collidine–acetate buffer, pH4·5, 0·17n-acetic acid and 0·1n-hydrochloric acid respectively. 4. With the exception of aspartic acid and glutamic acid, which are partly found in the acidic peptide fraction, the amino acids are completely separated from the peptides. 5. Contamination of the acidic peptide fraction with glutamic acid and aspartic acid can be largely avoided by previous addition of an excess of arginine. 6. Copper is removed from the eluates by extraction with 8-hydroxyquinoline in chloroform.

Métabolisme de l'acide indolyl-3-acétique dans les cultures de cellules d'Acer pseudoplatanus cultivés in vitro

1977 ◽  
Vol 55 (19) ◽  
pp. 2530-2534 ◽  
Author(s):  
F. Maillard ◽  
J.-P. Zrÿd
Keyword(s):  
Cell Wall ◽  
Acetic Acid ◽  
Culture Medium ◽  
Degradation Products ◽  
Cell Suspensions ◽  
The Other ◽  
Acer Pseudoplatanus ◽  
Kinetics Of

Incubation of cell suspensions of sycamore (Acer pseudoplatanus) with β-indoyl-3-acetic acid (IAA) first led to the formation of IAA-glycosides, then to that of IAA-aspartate. Great differences are observed between the kinetics of IAA transformed by two distinct strains: one, auxin dependent (S), the other, auxin independent (MB). Other degradation products are only found in the culture medium. The localization of IAA-degrading systems in the cell wall is postulated. The auxin requirement of the S strain is discussed.

Zur Genese Der Amidisch An Den Chromophor Von Pyoverdinen Gebundenen Dicarbonsäuren [1]

1991 ◽  
Vol 46 (5-6) ◽  
pp. 398-406 ◽  
Author(s):  
H. Schäfer ◽  
K. Taraz ◽  
H. Budzikiewicz
Keyword(s):  
Glutamic Acid ◽  
Dicarboxylic Acid ◽  
Succinic Acid ◽  
Pseudomonas Fluorescens ◽  
Exponential Growth ◽  
Culture Medium ◽  
Culture Time ◽  
Hydrolysis Of

Pseudomonas strains of the so-called fluorescent group usually produce several pyoverdins which differ only in the nature of a dicarboxylic acid bound amidically to the chromophor. For the pyoverdins isolated from the culture medium of Pseudomonas fluorescens 12 it is shown that succinic acid is an artefact formed by hydrolysis of succinic amide, and that a-ketoglutaric acid is transformed enzymatically to glutamic acid. This process is reversed after the phase of exponential growth of the bacteria. The ratio C4- vs. C5 -acids changes with the culture time and with increasing Fe3+ content of the medium in favor of the latter

scholarly journals Disease-related Metabolites in Culture Medium of Fibroblasts from Patients with d-2-Hydroxyglutaric Aciduria, l-2-Hydroxyglutaric Aciduria, and Combined d/l-2-Hydroxyglutaric Aciduria

2003 ◽  
Vol 49 (7) ◽  
pp. 1133-1138 ◽  
Author(s):  
Eduard A Struys ◽  
Nanda M Verhoeven ◽  
Birthe Roos ◽  
Cornelis Jakobs
Keyword(s):  
Cell Culture ◽  
Glutamic Acid ◽  
Cell Lines ◽  
Culture Medium ◽  
Culture Media ◽  
Control Cell ◽  
Fibroblast Cell ◽  
Gas Chromatography Mass Spectrometry ◽  
Organic Acidurias ◽  
Stable Isotope Dilution

Abstract Background: d-2-Hydroxyglutaric aciduria (D-2-HGA), l-2-hydroxyglutaric aciduria (L-2-HGA), and the combined d/l-2-hydroxyglutaric aciduria (D/L-2-HGA) are poorly understood organic acidurias. To investigate the usefulness of cultured human skin fibroblasts for both diagnostic and research purposes, we measured disease-related metabolites in the cell culture medium. Methods: We measured d-2-hydroxyglutarate (D-2-HG), l-2-hydroxyglutarate (L-2-HG), succinate, 2-ketoglutarate, and citrate in fibroblast cell medium by stable-isotope-dilution gas chromatography–mass spectrometry and glutamine, glutamic acid, and lysine with an amino acid analyzer. We used six cell lines from patients with D-2-HGA, two from patients with L-2-HGA, three from patients with D/L-2-HGA, and seven control cell lines. Culture medium was analyzed after a 96-h incubation period. Results: Culture media from cell lines from D-2-HGA patients contained D-2-HG at concentrations 5- to 30-fold higher than media from controls, whereas the concentration of L-2-HG in media was not increased. Media from L-2-HGA cell lines showed a fivefold increase in L-2-HG compared with controls. Media containing fibroblasts from D/L-2-HGA patients contained moderately increased amounts of both D-2-HG and L-2-HG. For all cell lines, succinate concentrations in the blank medium were higher than after 96 h of incubation with the exception of two of three D/L-2-HGA cell lines. Media of D-2-HGA cell lines had 2-ketoglutarate concentrations that were 40% of that for controls. Glutamic acid concentrations in media of these cell lines were 60% lower than in controls. Conclusions: Cell culture media from fibroblasts from patients with D-2-HGA, L-2-HGA, or D/L-2-HGA contain increased amounts the corresponding 2-HGs, demonstrating the suitability of fibroblasts for both diagnosis of and research concerning 2-HGAs.

scholarly journals Production of indole-3-acetic acid in the culture medium of microalga Scenedesmus obliquus (UTEX 393)

2011 ◽  
Vol 22 (12) ◽  
pp. 2355-2361 ◽  
Author(s):  
Rosa E. Prieto C. ◽  
Nancy M. Cordoba C. ◽  
Andres M. Montenegro J. ◽  
Gloria E. González-Mariño
Keyword(s):  
Acetic Acid ◽  
Culture Medium ◽  
Scenedesmus Obliquus ◽  
Indole 3 Acetic Acid
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