scholarly journals Bio-statistical enhancement of acyl transfer activity of amidase for biotransformation of N-substituted aromatic amides

2016 ◽  
Vol 62 (2) ◽  
pp. 90-97
Author(s):  
Ravi Kant Bhatia ◽  
Shashi Kant Bhatia ◽  
Praveen Kumar Mehta ◽  
Tek Chand Bhalla
Keyword(s):  
Acyl Transfer ◽  
Transfer Activity ◽  
Acyl Transfer Activity

scholarly journals A novel thermostable nitrilase superfamily amidase from Geobacillus pallidus showing acyl transfer activity

2007 ◽  
Vol 75 (4) ◽  
pp. 801-811 ◽  
Author(s):  
H. S. Makhongela ◽  
A. E. Glowacka ◽  
V. B. Agarkar ◽  
B. T. Sewell ◽  
B. Weber ◽  
...  
Keyword(s):  
Acyl Transfer ◽  
Transfer Activity ◽  
Acyl Transfer Activity ◽  
Geobacillus Pallidus

Bi-substrate Kinetic Analysis of Acyl Transfer Activity of Purified Amidase from Pseudomonas putida BR-1

2015 ◽  
Vol 145 (4) ◽  
pp. 1033-1040 ◽  
Author(s):  
Ravi Kant Bhatia ◽  
Shashi Kant Bhatia ◽  
Vijay Kumar ◽  
Tek Chand Bhalla
Keyword(s):  
Pseudomonas Putida ◽  
Kinetic Analysis ◽  
Acyl Transfer ◽  
Transfer Activity ◽  
Acyl Transfer Activity

scholarly journals Rational Design for Enhanced Acyltransferase Activity in Water Catalyzed by the Pyrobaculum calidifontis VA1 Esterase

2021 ◽  
Vol 9 (8) ◽  
pp. 1790
Author(s):  
Amanda Staudt ◽  
Henrik Terholsen ◽  
Jasmin Kaur ◽  
Henrik Müller ◽  
Simon P. Godehard ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Rational Design ◽  
Bulk Water ◽  
Acyl Transfer ◽  
Acyl Donor ◽  
Acyltransferase Activity ◽  
Pyrobaculum Calidifontis ◽  
Synthetic Strategy ◽  
Highly Active ◽  
Acyl Transfer Activity

Biocatalytic transesterification is commonly carried out employing lipases in anhydrous organic solvents since hydrolases usually prefer hydrolysis over acyl transfer in bulk water. However, some promiscuous acyltransferases can catalyze acylation in an aqueous solution. In this study, a rational design was performed to enhance the acyltransferase selectivity and substrate scope of the Pyrobaculum calidifontis VA1 esterase (PestE). PestE wild type and variants were applied for the acylation of monoterpene alcohols. The mutant PestE_I208A is selective for (–)-menthyl acetate (E-Value = 55). Highly active acyltransferases were designed, allowing for complete conversion of (–)-citronellol to citronellyl acetate. Additionally, carvacrol was acetylated but with lower conversions. To the best of our knowledge, this is the first example of the biocatalytic acylation of a phenolic alcohol in bulk water. In addition, a high citronellol conversion of 92% was achieved with the more environmentally friendly and inexpensive acyl donor ethyl acetate using PestE_N288F as a catalyst. PestE_N288F exhibits good acyl transfer activity in an aqueous medium and low hydrolysis activity at the same time. Thus, our study demonstrates an alternative synthetic strategy for acylation of compounds without organic solvents.

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