scholarly journals Microbial analysis of a composted product of marine animal resources and isolation of bacteria antagonistic to a plant pathogen from the compost

2008 ◽  
Vol 54 (3) ◽  
pp. 149-158 ◽  
Author(s):  
Chie Niisawa ◽  
Shin-ichiro Oka ◽  
Hiroaki Kodama ◽  
Mitsuyo Hirai ◽  
Yoshifumi Kumagai ◽  
...  
Keyword(s):  
Plant Pathogen ◽  
Marine Animal ◽  
Microbial Analysis ◽  
Animal Resources

scholarly journals Thermotolerant Bacillus kokeshiiformis sp. nov. isolated from marine animal resources compost

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2668-2674 ◽  
Author(s):  
Pramod Poudel ◽  
Hirokuni Miyamoto ◽  
Hisashi Miyamoto ◽  
Yuki Okugawa ◽  
Yukihiro Tashiro ◽  
...  
Keyword(s):  
Type Species ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Marine Animal ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Bacillus Niacini ◽  
Thermotolerant Bacterium ◽  
Animal Resources

A novel Gram-staining-positive, endospore-forming, rod-shaped, facultatively anaerobic, thermotolerant bacterium, designated strain MO-04T, was isolated from a marine animal resources (MAR) compost. The 16S rRNA gene sequence of strain MO-04T showed 99.4 % similarity with Bacillus thermolactis R-6488T, 94.1 % similarity with Bacillus thermoamylovorans CNCM I-1378T, 93.3 % similarity with Bacillus humi LMG 22167T, 93.2 % similarity with Bacillus niacini IFO 15566T and the similarities with other species were less than 93 %. DNA–DNA relatedness between strain MO-04T and B. thermolactis DSM 23332T was 45 %. The DNA G+C content of strain MO-04T was 33.4 mol%, comparatively lower than that of B. thermolactis R-6488T (35.0 mol%). Strain MO-04T grew at 35–61 °C (optimum 50 °C), pH 4.5–9.0 (optimum pH 7.2) and tolerated up to 8.0 % (w/v) NaCl (optimum 2 %). The MO-04T cell wall peptidoglycan type was meso-2,6-diaminopimelic acid, and the major fatty acids were C16 : 1, C14 : 1, C17 : 0 and C17 : 1. The major polar lipids were represented by diphosphatidylglycerol and phosphatidylglycerol and two unidentified phospholipids. The analysed polyphasic data presented here clearly indicate that the isolate MO-04T is considered to represent a novel species within the genus Bacillus for which the name Bacillus kokeshiiformis sp. nov. is proposed. The type strain of B. kokeshiiformis is MO-04T ( = JCM 19325T = KCTC 33163T).

Microbial Analysis and Test of Plant Pathogen Antagonism of Municipal and Farm Composts

2005 ◽  
Vol 22 (4) ◽  
pp. 349-367 ◽  
Author(s):  
Tanu Arora ◽  
Ylva Eklind ◽  
Birgitta Rämert ◽  
Sadhna Alström
Keyword(s):  
Plant Pathogen ◽  
Microbial Analysis

Analisis Kandungan Mikroba, Formalin, dan Timbal (Pb) pada Tahu Sumedang yang Dijual Di Daerah Macet Cicurug, Ciawi, dan Cisarua Jawa Barat

2020 ◽  
Vol 6 (1) ◽  
pp. 087
Author(s):  
Rosy Hutami ◽  
M Fakih Kurniawan ◽  
Henna Khoerunnisa
Keyword(s):  
Content Analysis ◽  
Microbial Contamination ◽  
Atomic Absorption Spectrophotometry ◽  
Plate Count ◽  
Traffic Jam ◽  
Absorption Spectrophotometry ◽  
Traffic Jams ◽  
Total Plate Count ◽  
Microbial Analysis ◽  
Aas Method

Sumedang tofu is one of favorite foods for Indonesian society. But many sellers or producers are not aware to the food safety of sumedang tofu. The aims of this study were to analyze the microbial, formalin, and lead (Pb) contents in ready-to-eat sumedang tofu which were sold in traffic jams area in Cicurug, Ciawi, and Cisarua. The analysis were carried out by Total Plate Count (TPC) testing for microbial analysis, potassium permanganate reaction (KMnO4) testing for formaldehyde analysis, and atomic absorption spectrophotometry (AAS) method for lead content analysis in the samples. The results obtained for the microbial analysis were sumedang tofu that were sold in the traffic jam areas of Cicurug, Ciawi, and Cisarua contained contaminant above the treshold (1.4 x 105 colonies / gram to 2.2 x 105 colonies / gram of microbes). All of the samples of sumedang tofu were positive containing formaldehyde. Otherwise, there were no lead (Pb) content in all samples regarding to AAS analysis. This study concluded that the ready-to-eat sumedang tofu those were sold in traffic jam area in the Cicurug, Ciawi, and Cisarua were not suitable for consumption because it contained exceed microbial contamination and formalin which are harmful for human health.Keywords : formalin, microbes, sumedang tofu, lead, traffic jam

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