scholarly journals Application of the hypervariable region of the 16S rDNA sequence as an index for the rapid identification of species in the genus Alicyclobacillus.

2002 ◽  
Vol 48 (5) ◽  
pp. 243-250 ◽  
Author(s):  
Keiichi Goto ◽  
Kaoru Mochida ◽  
Mika Asahara ◽  
Masayuki Suzuki ◽  
Akira Yokota
Keyword(s):  
16S Rdna ◽  
Hypervariable Region ◽  
Rapid Identification ◽  
Rdna Sequence ◽  
16S Rdna Sequence ◽  
Identification Of Species

scholarly journals Reclassification of Brevibacillus brevis strains NCIMB 13288 and DSM 6472 (=NRRL NRS-887) as Aneurinibacillus danicus sp. nov. and Brevibacillus limnophilus sp. nov.

2004 ◽  
Vol 54 (2) ◽  
pp. 419-427 ◽  
Author(s):  
Keiichi Goto ◽  
Rieko Fujita ◽  
Yuko Kato ◽  
Mika Asahara ◽  
Akira Yokota
Keyword(s):  
16S Rdna ◽  
Sequence Similarity ◽  
Hypervariable Region ◽  
Rdna Sequence ◽  
Bacterial Strains ◽  
16S Rdna Sequence ◽  
Dna Relatedness ◽  
Rdna Sequences ◽  
Bacillus Methanolicus ◽  
Brevibacillus Brevis

Comparison of the hypervariable region (269–279 bases in length) at the 5′ end of the 16S rDNA sequences of 29 bacterial strains that were identified previously as Brevibacillus brevis showed that 13 strains clustered with Aneurinibacillus species, eight strains clustered with Bacillus species and eight strains clustered with Brevibacillus species. Based on DNA–DNA hybridization results, 27 strains, not including [Brevibacillus brevis] NCIMB 13288 and [Brevibacillus brevis] DSM 6472, were reidentified as Aneurinibacillus migulanus, Aneurinibacillus thermoaerophilus, Bacillus methanolicus, Bacillus oleronius, Brevibacillus agri, Brevibacillus brevis and Brevibacillus parabrevis. [Brevibacillus brevis] NCIMB 13288, which was located in the Aneurinibacillus cluster, showed low DNA–DNA relatedness (<14 %) and low 16S rDNA sequence similarity (96·8–97·9 %) to other Aneurinibacillus species. [Brevibacillus brevis] DSM 6472, which was located in the Brevibacillus cluster, also showed low DNA–DNA relatedness (<12 %) and low 16S rDNA sequence similarity (95·4–98·8 %) to other Brevibacillus species. These genotypic and phylogenetic data, plus phenotypic and chemotaxonomic characteristics, suggest that [Brevibacillus brevis] NCIMB 13288 (=IAM 15048) and [Brevibacillus brevis] DSM 6472 (=NRRL NRS-887) represent novel species of the genera Aneurinibacillus and Brevibacillus, respectively, for which the names Aneurinibacillus danicus sp. nov. and Brevibacillus limnophilus sp. nov. are proposed.

16S rDNA Sequence based Phylogenetic Analysis of Some Bacterial Phytoplasma

2012 ◽  
Vol 3 (3) ◽  
pp. 302-304
Author(s):  
G. D.Sharma G. D.Sharma ◽  
◽  
* Dhritiman Chanda ◽  
D.K. Jha D.K. Jha
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rdna ◽  
Rdna Sequence ◽  
16S Rdna Sequence

scholarly journals Erratum to: Deodorization of pig slurry and characterization of bacterial diversity using 16S rDNA sequence analysis

2014 ◽  
Vol 52 (12) ◽  
pp. 1056-1056
Author(s):  
Ok-Hwa Hwang ◽  
Sebastian Raveendar ◽  
Young-Ju Kim ◽  
Ji-Hun Kim ◽  
Tae-Hun Kim ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Bacterial Diversity ◽  
16S Rdna ◽  
Rdna Sequence ◽  
Pig Slurry ◽  
16S Rdna Sequence ◽  
16S Rdna Sequence Analysis

scholarly journals Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of ‘Brevibacterium helvolum’ DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov.

2004 ◽  
Vol 54 (3) ◽  
pp. 783-789 ◽  
Author(s):  
Célia M. Manaia ◽  
Balbina Nogales ◽  
Norbert Weiss ◽  
Olga C. Nunes
Keyword(s):  
Cell Wall ◽  
16S Rdna ◽  
Sequence Similarity ◽  
Rdna Sequence ◽  
Strictly Aerobic ◽  
16S Rdna Sequence ◽  
Cell Wall Peptidoglycan ◽  
16S Rdna Sequence Analysis ◽  
Degrading Bacterium ◽  
Phylogenetic Level

A Gram-positive, molinate-degrading bacterium, strain ON4T (=DSM 13485T=LMG 21909T), was isolated from a mixed bacterial culture able to mineralize the herbicide molinate. The strain was strictly aerobic, oxidase- and catalase-positive and non-acid-fast, with a growth temperature of 10–41 °C. It contained the major menaquinone MK-9 and a cell-wall peptidoglycan based on d-ornithine. 16S rDNA sequence analysis revealed that the strain formed a distinct line of descent in the family Microbacteriaceae, showing the highest 16S rDNA similarity (∼95 %) to members of the genus Curtobacterium and ‘Brevibacterium helvolum’ DSM 20419 (=ATCC 13715). The latter was reported to have the cell-wall peptidoglycan type B2γ and the major menaquinone MK-9, which are typical of Clavibacter, but it is clearly separated from this genus at the phylogenetic level. Based on low values of 16S rDNA sequence similarity to previously described genera and their distinctive phenotypic characteristics, it is proposed that strains ON4T and ‘B. helvolum’ DSM 20419 be classified as two novel genera and species, with the respective names Gulosibacter molinativorax gen. nov., sp. nov. and Pseudoclavibater helvolus gen. nov., sp. nov.

scholarly journals Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir

2004 ◽  
Vol 54 (3) ◽  
pp. 827-835 ◽  
Author(s):  
Ying Li ◽  
Yoshiaki Kawamura ◽  
Nagatoshi Fujiwara ◽  
Takashi Naka ◽  
Hongsheng Liu ◽  
...  
Keyword(s):  
16S Rdna ◽  
Sequence Similarity ◽  
Rhodococcus Erythropolis ◽  
Rdna Sequence ◽  
Taxonomic Study ◽  
16S Rdna Sequence ◽  
Gram Positive Bacteria ◽  
16S Rdna Sequence Analysis ◽  
Rothia Mucilaginosa ◽  
Established Species

Four Gram-positive bacteria, strains A1-17BT, A1-22T, A1-3T and A1-8, isolated from the air in the Russian space laboratory Mir, were subjected to a polyphasic taxonomic study. Phylogenetic analysis of the bacteria based on their 16S rDNA sequence showed that they belong to the genera Rothia (A1-17BT), Rhodococcus (A1-22T) and Arthrobacter (A1-3T and A1-8). Morphological, physiological, chemotaxonomic and genomic characteristics supported the assignments of these strains to these genera, but they could not be classified as any existing species within each respective genus. 16S rDNA similarity values between strain A1-17BT and its neighbours, Rothia dentocariosa genomovar II, Rothia dentocariosa, Rothia mucilaginosa and Rothia nasimurium, were respectively 99·8, 98·0, 96·4 and 95·4 %. Polyphasic taxonomic evidence indicated that strain A1-17BT should be categorized together with the unofficially named Rothia dentocariosa genomovar II, but clearly differentiated them from the established species of the genus Rothia. Strain A1-22T formed a coherent cluster with Rhodococcus erythropolis, Rhodococcus globerulus, Rhodococcus marinonascens and Rhodococcus percolatus in 16S rDNA sequence analysis, but DNA–DNA relatedness values were only 45·5, 35·3, 18·9 and 21·9 %. Strains A1-3T and A1-8 shared 99·9 % 16S rDNA sequence similarity, and strain A1-3T showed the highest level of 16S rDNA similarity, 96·6 %, to Arthrobacter polychromogenes. Contrasting biochemical characteristics were also identified. Finally, as a result of the polyphasic taxonomic study, three of the strains are proposed as type strains of novel species: Rothia aeria sp. nov. (A1-17BT=GTC 867T=JCM 11412T=DSM 14556T), Rhodococcus baikonurensis sp. nov. (A1-22T=GTC 1041T=JCM 11411T=DSM 44587T) and Arthrobacter russicus sp. nov. (A1-3T=GTC 863T=JCM 11414T=DSM 14555T).

A new chlamydia-like 16S rDNA sequence from a clinical sample

Microbiology ◽  
2001 ◽  
Vol 147 (3) ◽  
pp. 515-516 ◽  
Author(s):  
Daniele Corsaro ◽  
Danielle Venditti ◽  
Alain Le Faou ◽  
Paolo Guglielmetti ◽  
Marcello Valassina
Keyword(s):  
16S Rdna ◽  
Clinical Sample ◽  
Rdna Sequence ◽  
16S Rdna Sequence
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