scholarly journals Vitamin requirement of sulfate-reducers isolated from sewage digestor fluids.

1981 ◽  
Vol 27 (3) ◽  
pp. 229-237 ◽  
Author(s):  
ATSUKO UEKI ◽  
TSUNEJI SUTO
Keyword(s):  
Sulfate Reducers ◽  
Vitamin Requirement

scholarly journals Two Marine Desulfotomaculum spp. of Different Origin are Capable of Utilizing Acetone and Higher Ketones

2021 ◽  
Author(s):  
Jasmin Frey ◽  
Sophie Kaßner ◽  
Bernhard Schink
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Thiamine Diphosphate ◽  
Sulfate Reducers ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria ◽  
Different Origin

AbstractDegradation of acetone and higher ketones has been described in detail for aerobic and nitrate-reducing bacteria. Among sulfate-reducing bacteria, degradation of acetone and other ketones is still an uncommon ability and has not been understood completely yet. In the present work, we show that Desulfotomaculum arcticum and Desulfotomaculum geothermicum are able to degrade acetone and butanone. Total proteomics of cell-free extracts of both organisms indicated an involvement of a thiamine diphosphate-dependent enzyme, a B12-dependent mutase, and a specific dehydrogenase during acetone degradation. Similar enzymes were recently described to be involved in acetone degradation by Desulfococcus biacutus. As there are so far only two described sulfate reducers able to degrade acetone, D. arcticum and D. geothermicum represent two further species with this capacity. All these bacteria appear to degrade acetone via the same set of enzymes and therefore via the same pathway.

scholarly journals An oligotrophic deep-subsurface community dependent on syntrophy is dominated by sulfur-driven autotrophic denitrifiers

2016 ◽  
Vol 113 (49) ◽  
pp. E7927-E7936 ◽  
Author(s):  
Maggie C. Y. Lau ◽  
Thomas L. Kieft ◽  
Olukayode Kuloyo ◽  
Borja Linage-Alvarez ◽  
Esta van Heerden ◽  
...  
Keyword(s):  
Deep Subsurface ◽  
Sulfate Reducers ◽  
Paradoxical Situation ◽  
Gain Energy ◽  
Total Community ◽  
Diverse Community ◽  
Sulfur Oxidizing ◽  
Total Dna ◽  
Microbial Groups ◽  
Energy Processes

Subsurface lithoautotrophic microbial ecosystems (SLiMEs) under oligotrophic conditions are typically supported by H2. Methanogens and sulfate reducers, and the respective energy processes, are thought to be the dominant players and have been the research foci. Recent investigations showed that, in some deep, fluid-filled fractures in the Witwatersrand Basin, South Africa, methanogens contribute <5% of the total DNA and appear to produce sufficient CH4to support the rest of the diverse community. This paradoxical situation reflects our lack of knowledge about the in situ metabolic diversity and the overall ecological trophic structure of SLiMEs. Here, we show the active metabolic processes and interactions in one of these communities by combining metatranscriptomic assemblies, metaproteomic and stable isotopic data, and thermodynamic modeling. Dominating the active community are four autotrophic β-proteobacterial genera that are capable of oxidizing sulfur by denitrification, a process that was previously unnoticed in the deep subsurface. They co-occur with sulfate reducers, anaerobic methane oxidizers, and methanogens, which each comprise <5% of the total community. Syntrophic interactions between these microbial groups remove thermodynamic bottlenecks and enable diverse metabolic reactions to occur under the oligotrophic conditions that dominate in the subsurface. The dominance of sulfur oxidizers is explained by the availability of electron donors and acceptors to these microorganisms and the ability of sulfur-oxidizing denitrifiers to gain energy through concomitant S and H2oxidation. We demonstrate that SLiMEs support taxonomically and metabolically diverse microorganisms, which, through developing syntrophic partnerships, overcome thermodynamic barriers imposed by the environmental conditions in the deep subsurface.

scholarly journals Increasing sulfate levels show a differential impact on synthetic communities comprising different methanogens and a sulfate reducer

2019 ◽  
Vol 16 (154) ◽  
pp. 20190129 ◽  
Author(s):  
Jing Chen ◽  
Matthew J. Wade ◽  
Jan Dolfing ◽  
Orkun S. Soyer
Keyword(s):  
Microbial Communities ◽  
Methane Production ◽  
System Stability ◽  
Sulfate Reducer ◽  
Differential Impact ◽  
Sulfate Reducers ◽  
Hydrogenotrophic Methanogens ◽  
The Stability ◽  
The Impact

Methane-producing microbial communities are of ecological and biotechnological interest. Syntrophic interactions among sulfate reducers and aceto/hydrogenotrophic and obligate hydrogenotrophic methanogens form a key component of these communities, yet, the impact of these different syntrophic routes on methane production and their stability against sulfate availability are not well understood. Here, we construct model synthetic communities using a sulfate reducer and two types of methanogens representing different methanogenesis routes. We find that tri-cultures with both routes increase methane production by almost twofold compared to co-cultures and are stable in the absence of sulfate. With increasing sulfate, system stability and productivity decreases and does so faster in communities with aceto/hydrogenotrophic methanogens despite the continued presence of acetate. We show that this is due to a shift in the metabolism of these methanogens towards co-utilization of hydrogen with acetate. These findings indicate the important role of hydrogen dynamics in the stability and productivity of syntrophic communities.

Bioelectrocatalyzed reduction of acetic and butyric acids via direct electron transfer using a mixed culture of sulfate-reducers drives electrosynthesis of alcohols and acetone

2013 ◽  
Vol 49 (58) ◽  
pp. 6495 ◽  
Author(s):  
Mohita Sharma ◽  
Nabin Aryal ◽  
Priyangshu M. Sarma ◽  
Karolien Vanbroekhoven ◽  
Banwari Lal ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Mixed Culture ◽  
Direct Electron Transfer ◽  
Sulfate Reducers
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