scholarly journals Viral Transgene Expression Delivered by Repeat Intraocular Adenoviral Vector Injection: in Vivo Live Imaging Study

2012 ◽  
Vol 11 (5) ◽  
pp. 7290.2011.00053 ◽  
Author(s):  
Peter L. Gehlbach ◽  
Roy S. Chuck ◽  
Chung-Gyu Park ◽  
Choul Yong Park
Keyword(s):  
Transgene Expression ◽  
Adenoviral Vector ◽  
Imaging Study ◽  
Live Imaging

Tumor-selective transgene expression in vivo mediated by an E2F-responsive adenoviral vector

1997 ◽  
Vol 3 (10) ◽  
pp. 1145-1149 ◽  
Author(s):  
Michael J. Parr ◽  
Yoshinobu Manome ◽  
Toshihide Tanaka ◽  
Patrick Wen ◽  
Donald W. Kufe ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Adenoviral Vector ◽  
Tumor Selective

scholarly journals Episomal Persistence of Recombinant Adenoviral Vector Genomes during the Cell Cycle In Vivo

2003 ◽  
Vol 77 (13) ◽  
pp. 7689-7695 ◽  
Author(s):  
Anja Ehrhardt ◽  
Hui Xu ◽  
Mark A. Kay
Keyword(s):  
Transgene Expression ◽  
Adenoviral Vector ◽  
First Generation ◽  
Expression Levels ◽  
Copy Numbers ◽  
Immune Mediated ◽  
Cell Cycling ◽  
Dna Copy Numbers

ABSTRACT Previously we showed that recombinant adenoviral helper-dependent (HD) vectors result in long-term transgene expression levels in vivo which slowly declined by 95% over a period of 1 year. In this study, we further establish that this was not predominantly immune mediated. To determine if cell turnover was responsible for the loss of transgene expression, we induced rapid hepatocyte cell cycling in mouse liver, by performing a surgical two-thirds partial hepatectomy. We observed a 55 and 65% reduction in transgene expression levels and a 50 and 71% loss of vector genomes for the HD vector and the first-generation adenoviral vector. In sharp contrast, in nonviral, episomal plasmid DNA-injected mice, transgene expression levels and DNA copy numbers decreased by 95 and 99%, respectively. These findings suggest that cell division alone was not the primary reason for the slow decrease in transgene expression levels and that recombinant adenoviral vectors have a more robust mechanism for maintaining persistence during cell cycling. Several potential mechanisms are proposed.

scholarly journals Optimization of adenoviral vector-mediated transgene expression in the canine brain in vivo, and in canine glioma cells in vitro

2007 ◽  
Vol 9 (3) ◽  
pp. 245-258 ◽  
Author(s):  
Marianela Candolfi ◽  
G. Elizabeth Pluhar ◽  
Kurt Kroeger ◽  
Mariana Puntel ◽  
James Curtin ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Adenoviral Vector ◽  
Glioma Cells ◽  
Canine Brain

scholarly journals In vivo transgene expression from an adenoviral vector is altered following a 6-OHDA lesion of the dopamine system

2005 ◽  
Vol 137 (1-2) ◽  
pp. 1-10 ◽  
Author(s):  
E.M. Torres ◽  
C. Monville ◽  
P.R. Lowenstein ◽  
M.G. Castro ◽  
S.B. Dunnett
Keyword(s):  
Transgene Expression ◽  
Adenoviral Vector ◽  
Dopamine System
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