Phosphate Analysis of Anthropic Soils

1976 ◽  
Vol 3 (4) ◽  
pp. 447 ◽  
Author(s):  
Alf Sjöberg ◽  
Alf Sjoberg
2020 ◽  
Vol 20 (2) ◽  
pp. 395
Author(s):  
Ardina Purnama Tirta ◽  
Asep Saefumillah ◽  
Foliatini Foliatini ◽  
Herawati Herawati

The phenomenon of phosphate release in sediments into water bodies under oxic environment has been investigated using the Diffusive Gradient in Thin Film (DGT) technique. This research consists of several stages: polymer synthesis and DGT probe assembly, sediment sampling, DGT deployment in oxic conditions, and phosphate analysis from DGT adsorption results. Acrylamide polymer was successfully synthesized with a composition 15% acrylamide; N-N'-methylenebisacrylamide 0.1% and ferrihydrite as binding gels. DGT probes were assembly by placing a 16 x 3.2 cm polyacrylamide gel, binding gels and filter membranes on the DGT probes. The sediment sample was taken from the Bogor Botanical Gardens at the coordinates 6°36’00.6” S; 106°47’51.0” E. The DGT probe was placed in sediment samples for 1, 3 and 7 days in oxic conditions. After the prescribed time, the binding gel was removed and cut every 1 cm depth, then eluted using 0.25 M H2SO4 and the phosphate concentration was measured using spectrophotometry method. The results showed that the phosphate concentration tends to be higher with the increasing incubation time and depth. Maximum CDGT phosphate released on day 1, day 3 and day 7 were 1.00 µg/L at a depth of 14 cm, 6.61 µg/L at a depth of 14 cm, and 20.92 µg/L at a depth of 11 cm respectively. This ensures that the phosphate in water bodies comes from biogeochemical processes that occur in sediments and is successfully measured through DGT techniques.


1967 ◽  
Vol 18 (2) ◽  
pp. 322-324 ◽  
Author(s):  
Carl Hirsch ◽  
Sherman Kupfer ◽  
Irwin Oreskes

Author(s):  
Allan E. Hewitt ◽  
Megan R. Balks ◽  
David J. Lowe
Keyword(s):  

1982 ◽  
Vol 48 (S1) ◽  
pp. 50-57
Author(s):  
N D Balaam ◽  
H M Porter

The principal method adopted for the soil phosphate analysis for the Shaugh Moor project was a slightly modified version of that published by Dick and Tabatabai (1977). This method involves extraction of soil phosphate by alkaline oxidation with Sodium hypobromite (NaOBr-NaOh). It has been calculated that this process extracts about 60-70% of total phosphate from soils such as those on Shaugh Moor (Ralph, pers comm). This was considered satisfactory foe the purposes of this project.It is thought that this method has not been used before in archaeological survey work but it is suggested that the simplicity of the technique and the relatively high level of phosphate extraction might prove of value in future work.


Author(s):  
Thea Kirana ◽  
Heriandi Sutadi ◽  
Sarworini B Budiardjo

 Objective: The objective of this study is to analyze the differences in dental calculus indices and calcium and phosphate levels in children with chronic kidney disease undergoing hemodialysis (HD) and peritoneal dialysis (PD) therapy.Methods: In this cross-sectional study, 15 children undergoing HD therapy and 15 undergoing PD therapy were subjected to intraoral examination for dental calculus indices. Whole unstimulated saliva samples of all subjects were collected and subjected to calcium and phosphate analysis. Statistical analyses were conducted using the Mann–Whitney and independent t-tests.Results: The dental calculus indices were significantly lower and salivary calcium level was significantly higher in the PD group than those in the HD group (p<0.05). The salivary phosphate level was lower in the PD group (p≥0.05) but without significance.Conclusion: The PD group had lower dental calculus indices and higher salivary calcium level. The higher salivary phosphate levels in the HD group may have contribute to the higher dental calculus indices, probably because phosphate plays an important role in dental calculus formation.


2020 ◽  
Vol 11 (5) ◽  
pp. 511-523
Author(s):  
Bruno Campos Mantovanelli ◽  
Leandro Coutinho Alho ◽  
Milton César Costa Campos ◽  
José Mauricio da Cunha ◽  
Ivanildo Amorim Oliveira
Keyword(s):  

2011 ◽  
Vol 10 (7) ◽  
pp. 985-997 ◽  
Author(s):  
Karina Mariño ◽  
M. Lucia Sampaio Güther ◽  
Amy K. Wernimont ◽  
Wei Qiu ◽  
Raymond Hui ◽  
...  

ABSTRACT A gene predicted to encode Trypanosoma brucei glucosamine 6-phosphate N -acetyltransferase ( TbGNA1 ; EC 2.3.1.4) was cloned and expressed in Escherichia coli . The recombinant protein was enzymatically active, and its high-resolution crystal structure was obtained at 1.86 Å. Endogenous TbGNA1 protein was localized to the peroxisome-like microbody, the glycosome. A bloodstream-form T. brucei GNA1 conditional null mutant was constructed and shown to be unable to sustain growth in vitro under nonpermissive conditions, demonstrating that there are no metabolic or nutritional routes to UDP-GlcNAc other than via GlcNAc-6-phosphate. Analysis of the protein glycosylation phenotype of the TbGNA1 mutant under nonpermissive conditions revealed that poly- N -acetyllactosamine structures were greatly reduced in the parasite and that the glycosylation profile of the principal parasite surface coat component, the variant surface glycoprotein (VSG), was modified. The significance of results and the potential of TbGNA1 as a novel drug target for African sleeping sickness are discussed.


1962 ◽  
Vol 4 (5) ◽  
pp. 420-421 ◽  
Author(s):  
Amedeo F. D'Adamo ◽  
John R. Broich

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