Human Serum as a Stabilizer of Scarlet Fever Streptococcus Toxin Diluted for the Dick Test

1939 ◽  
Vol 54 (48) ◽  
pp. 2131
Author(s):  
Milton V. Veldee
Keyword(s):  
Human Serum ◽  
Scarlet Fever

scholarly journals OBSERVATIONS ON THE PRESENCE OF A TOXIC SUBSTANCE IN THE BLOOD AND URINE OF PATIENTS WITH SCARLET FEVER

1924 ◽  
Vol 40 (3) ◽  
pp. 381-395 ◽  
Author(s):  
James D. Trask ◽  
Francis G. Blake
Keyword(s):  
Human Serum ◽  
Scarlet Fever ◽  
Horse Serum ◽  
Toxic Substance ◽  
Local Infection ◽  
Hemolytic Streptococcus ◽  
Normal Horse Serum

A series of observations on the blood of patients acutely ill with scarlet fever has shown that a toxic substance can be demonstrated in the serum by means of intracutaneous injections of the serum in persons who have not had scarlet fever and whose serums fail to blanch the rash in scarlet fever. The reaction caused by this substance consists of a bright red local erythema, varying from 20 to 70 mm. in diameter, of 1 to 4 days duration. The severer reactions are moderately indurated and tender, and are followed bypigmentation and desquamation. Control injections in persons whose serums blanch the rash in scarlet fever cause no reaction. The toxic substance is not neutralized by mixture with a human serum which gives a negative blanching test but is readily neutralized by a human serum which gives a positive blanching test. It is not neutralized by normal horse serum, but is completely neutralized by Dochez's scarlatinal antistreptococcic serum. In a limited number of observations on the urine of patients with scarlet fever a similar toxic substance has been found in two out of five cases studied. Since the toxic substance described appears to resemble the toxic substance found in the filtrates of scarlatinal hemolytic streptococcus cultures by Dick and Dick and since it is neutralized not only by a blanching human serum but also by Dochez's scarlatinal antistreptococcic horse serum, the experiments reported support the conception that scarlet fever is a local infection of the throat by a particular type of Streptococcus hæmolyticus capable of producing a toxin which is absorbed and is the cause of the general manifestations of the disease.

scholarly journals COMPLEMENT DEVIATION IN SCARLET FEVER WITH COMPARATIVE STUDIES OF THE WASSERMANN AND NOGUCHI SYSTEMS

1911 ◽  
Vol 14 (3) ◽  
pp. 235-243 ◽  
Author(s):  
John A. Kolmer
Keyword(s):  
Human Serum ◽  
Comparative Studies ◽  
Scarlet Fever ◽  
Per Se ◽  
Wassermann Reaction

The Wassermann reaction in scarlet fever per se is uniformly negative. The antigen of scarlet fever liver yields practically the same results as that of luetic liver, and both fail to deviate complement with scarlet fever antibodies. The Noguchi reaction in scarlet fever is practically negative. Sixteen cases, or 6.4 per cent. of 250 cases, were positive when active serum was used; with inactivated serum, but eleven, or 4.4 per cent., remained positive. Five of these eleven cases were also positive with the Wassermann system. In other words, sixteen cases, or 6.4 per cent., were positive according to the Noguchi system with active or inactivated serum or both, whereas with the Wassermann system only 2 per cent. were positive. The presence of anti-sheep hemolysin normally in human serum is one of the main disturbing factors in the Wassermann system; for this reason, complement and hemolysin (made by immunization of rabbits) require careful titration. A positive Wassermann reaction usually indicates the presence of syphilitic antibodies, and a negative Noguchi reaction, their absence, and both systems should be used in the examination of all cases.

Development of a method to determine caffeine in human serum by HPLC

2015 ◽  
Vol 48 (06) ◽  
Author(s):  
C Rothammer ◽  
E Haen
Keyword(s):  
Human Serum
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