Sawmills in the Redwoods: Logging on the San Francisco Peninsula, 1849–1967. By Frank M. Stanger. (San Mateo: San Mateo County Historical Association, 1967. vii, 160 pp. Illustrations, maps, bibliography, index $9.50)

1968 ◽  
Vol 12 (1) ◽  
pp. 24-24
Author(s):  
Thomas R. Cox
Keyword(s):  
San Francisco ◽  
San Mateo County

Demography of the San Francisco Gartersnake in Coastal San Mateo County, California

2011 ◽  
Vol 2 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Brian J. Halstead ◽  
Glenn D. Wylie ◽  
Melissa Amarello ◽  
Jeffrey J. Smith ◽  
Michelle E. Thompson ◽  
...  
Keyword(s):  
San Francisco ◽  
Survival Rates ◽  
Thamnophis Sirtalis ◽  
Annual Survival ◽  
Coastal Prairie ◽  
Probability Of Survival ◽  
Annual Probability ◽  
The Status ◽  
San Mateo County

Abstract The San Francisco gartersnake Thamnophis sirtalis tetrataenia has been federally listed as endangered since 1967, but little demographic information exists for this species. We examined the demography of a San Francisco gartersnake population on approximately 213 ha of California coastal prairie in San Mateo County, California, from 2007 to 2010. The best-supported mark–recapture model indicated annual variation in daily capture probabilities and annual survival rates. Abundance increased throughout the study period, with a mean total population from 2008 to 2010 of 443 (95% CI  =  313–646) individuals. Annual survival was slightly greater than that of most other gartersnakes, with an annual probability of survival of 0.78 (0.55–0.95) in 2008–2009 and 0.75 (0.49–0.93) in 2009–2010. Mean annual per capita recruitment rates were 0.73 (0.02–2.50) in 2008–2009 and 0.47 (0.02–1.42) in 2009–2010. From 2008 to 2010, the probability of an increase in abundance at this site was 0.873, with an estimated increase of 115 (−82 to 326) individuals. The estimated population growth rate in 2008–2009 was 1.52 (0.73–3.29) and in 2009–2010 was 1.21 (0.70–2.17). Although this population is probably stable or increasing in the short term, long-term studies of the status of the San Francisco gartersnake at other sites are required to estimate population trends and to elucidate mechanisms that promote the recovery of this charismatic member of our native herpetofauna.

Ultrastructural Localization of Antigens by Capillary Action Immunocytochemistry

1996 ◽  
Vol 54 ◽  
pp. 40-41
Author(s):  
László G. Kömüves
Keyword(s):  
San Francisco ◽  
Colloidal Gold ◽  
Specific Binding ◽  
Ultrastructural Localization ◽  
Ultrastructural Level ◽  
Uranyl Acetate ◽  
Adhesive Tape ◽  
Distilled Water ◽  
Capillary Action ◽  
Rabbit Igg

Light microscopic immunohistochemistry based on the principle of capillary action staining is a widely used method to localize antigens. Capillary action immunostaining, however, has not been tested or applied to detect antigens at the ultrastructural level. The aim of this work was to establish a capillary action staining method for localization of intracellular antigens, using colloidal gold probes.Post-embedding capillary action immunocytochemistry was used to detect maternal IgG in the small intestine of newborn suckling piglets. Pieces of the jejunum of newborn piglets suckled for 12 h were fixed and embedded into LR White resin. Sections on nickel grids were secured on a capillary action glass slide (100 μm wide capillary gap, Bio-Tek Solutions, Santa Barbara CA, distributed by CMS, Houston, TX) by double sided adhesive tape. Immunolabeling was performed by applying reagents over the grids using capillary action and removing reagents by blotting on filter paper. Reagents for capillary action staining were from Biomeda (Foster City, CA). The following steps were performed: 1) wet the surface of the sections with automation buffer twice, 5 min each; 2) block non-specific binding sites with tissue conditioner, 10 min; 3) apply first antibody (affinity-purified rabbit anti-porcine IgG, Sigma Chem. Co., St. Louis, MO), diluted in probe diluent, 1 hour; 4) wash with automation buffer three times, 5 min each; 5) apply gold probe (goat anti-rabbit IgG conjugated to 10 nm colloidal gold, Zymed Laboratories, South San Francisco, CA) diluted in probe diluent, 30 min; 6) wash with automation buffer three times, 5 min each; 7) post-fix with 5% glutaraldehyde in PBS for 10 min; 8) wash with PBS twice, 5 min each; 9) contrast with 1% OSO4 in PBS for 15 min; 10) wash with PBS followed by distilled water for5 min each; 11) stain with 2% uranyl acetate for 10 min; 12) stain with lead citrate for 2 min; 13) wash with distilled water three times, 1 min each. The glass slides were separated, and the grids were air-dried, then removed from the adhesive tape. The following controls were used to ensure the specificity of labeling: i) omission of the first antibody; ii) normal rabbit IgG in lieu of first antibody; iii) rabbit anti-porcine IgG absorbed with porcine IgG.

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