Vacuolar Storage Proteins and the Putative Vacuolar Sorting Receptor BP-80 Exit the Golgi Apparatus of Developing Pea Cotyledons in Different Transport Vesicles

1999 ◽  
Vol 11 (8) ◽  
pp. 1509 ◽  
Author(s):  
Giselbert Hinz ◽  
Stefan Hillmer ◽  
Matthias Baumer ◽  
Inge Hohl
Keyword(s):  
Golgi Apparatus ◽  
Storage Proteins ◽  
Transport Vesicles ◽  
Vacuolar Sorting Receptor ◽  
Vacuolar Sorting ◽  
Sorting Receptor

scholarly journals Vacuolar sorting receptor for seed storage proteins in Arabidopsis thaliana

2003 ◽  
Vol 100 (26) ◽  
pp. 16095-16100 ◽  
Author(s):  
T. Shimada ◽  
K. Fuji ◽  
K. Tamura ◽  
M. Kondo ◽  
M. Nishimura ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Vacuolar Sorting Receptor ◽  
Vacuolar Sorting ◽  
Sorting Receptor

scholarly journals Vacuolar Storage Proteins Are Sorted in the Cis-Cisternae of the Pea Cotyledon Golgi Apparatus

2001 ◽  
Vol 152 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Stefan Hillmer ◽  
Ali Movafeghi ◽  
David G. Robinson ◽  
Giselbert Hinz
Keyword(s):  
Golgi Apparatus ◽  
Storage Proteins ◽  
Spatial Separation ◽  
Golgi Stack ◽  
Protein Storage ◽  
Protein Storage Vacuole ◽  
Vacuolar Sorting ◽  
Sorting Receptor ◽  
Storage Vacuole ◽  
Dense Vesicles

Developing pea cotyledons contain functionally different vacuoles, a protein storage vacuole and a lytic vacuole. Lumenal as well as membrane proteins of the protein storage vacuole exit the Golgi apparatus in dense vesicles rather than in clathrin-coated vesicles (CCVs). Although the sorting receptor for vacuolar hydrolases BP-80 is present in CCVs, it is not detectable in dense vesicles. To localize these different vacuolar sorting events in the Golgi, we have compared the distribution of vacuolar storage proteins and of α-TIP, a membrane protein of the protein storage vacuole, with the distribution of the vacuolar sorting receptor BP-80 across the Golgi stack. Analysis of immunogold labeling from cryosections and from high pressure frozen samples has revealed a steep gradient in the distribution of the storage proteins within the Golgi stack. Intense labeling for storage proteins was registered for the cis-cisternae, contrasting with very low labeling for these antigens in the trans-cisternae. The distribution of BP-80 was the reverse, showing a peak in the trans-Golgi network with very low labeling of the cis-cisternae. These results indicate a spatial separation of different vacuolar sorting events in the Golgi apparatus of developing pea cotyledons.

scholarly journals A Vacuolar Sorting Receptor PV72 on the Membrane of Vesicles that Accumulate Precursors of Seed Storage Proteins (PAC Vesicles)

2002 ◽  
Vol 43 (10) ◽  
pp. 1086-1095 ◽  
Author(s):  
Tomoo Shimada ◽  
Etsuko Watanabe ◽  
Kentaro Tamura ◽  
Yasuko Hayashi ◽  
Mikio Nishimura ◽  
...  
Keyword(s):  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Vacuolar Sorting Receptor ◽  
Vacuolar Sorting ◽  
Sorting Receptor

scholarly journals Demonstration in Yeast of the Function of BP-80, a Putative Plant Vacuolar Sorting Receptor

2001 ◽  
Vol 13 (4) ◽  
pp. 781-792 ◽  
Author(s):  
David Humair ◽  
Doramys Hernández Felipe ◽  
Jean-Marc Neuhaus ◽  
Nadine Paris
Keyword(s):  
Vacuolar Sorting Receptor ◽  
Vacuolar Sorting ◽  
Sorting Receptor
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