Genetic Interactions between the Chlorate-Resistant Mutant cr88 and the Photomorphogenic Mutants cop1 and hy5

2000 ◽  
Vol 12 (2) ◽  
pp. 199
Author(s):  
Dongsun Cao ◽  
Yun Lin ◽  
Chi-Lien Cheng
Keyword(s):  
Resistant Mutant ◽  
Genetic Interactions ◽  
Photomorphogenic Mutants ◽  
Chlorate Resistant Mutant

scholarly journals Nitrate reduction mutants of fusarium moniliforme (gibberella fujikuroi).

Genetics ◽  
1988 ◽  
Vol 118 (3) ◽  
pp. 417-423
Author(s):  
C Klittich ◽  
J F Leslie
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reduction ◽  
Fusarium Moniliforme ◽  
Reductase Activity ◽  
Regulatory Gene ◽  
Resistant Mutant ◽  
Gibberella Fujikuroi ◽  
Mutant Type ◽  
Nit Mutants ◽  
Chlorate Resistant Mutant

Abstract Twelve strains of Fusarium moniliforme were examined for their ability to sector spontaneously on toxic chlorate medium. All strains sectored frequently; 91% of over 1200 colonies examined formed chlorate-resistant, mutant sectors. Most of these mutants had lesions in the nitrate reduction pathway and were unable to utilize nitrate (nit mutants). nit mutations occurred in seven loci: a structural gene for nitrate reductase (nit1), a regulatory gene specific for the nitrate reduction pathway (nit3), and five genes controlling the production of a molybdenum-containing cofactor that is necessary for nitrate reductase activity (nit2, nit4, nit5, nit6, nit7). No mutations affecting nitrite reductase or a major nitrogen regulatory locus were found among over 1000 nit mutants. Mutations of nit1 were recovered most frequently (39-66%, depending on the strain) followed by nit3 mutations (23-42%). The frequency of isolation of each mutant type could be altered, however, by changing the source of nitrogen in the chlorate medium. We concluded that genetic control of nitrate reduction in F. moniliforme is similar to that in Aspergillus and Neurospora, but that the overall regulation of nitrogen metabolism may be different.

Sign in / Sign up

Export Citation Format

Share Document