Disruption of Interfascicular Fiber Differentiation in an Arabidopsis Mutant

Abstract Salicylic acid (SA) is required for resistance to many diseases in higher plants. SA-dependent cell death and defense-related responses have been correlated with disease resistance. The accelerated cell death 5 mutant of Arabidopsis provides additional genetic evidence that SA regulates cell death and defense-related responses. However, in acd5, these events are uncoupled from disease resistance. acd5 plants are more susceptible to Pseudomonas syringae early in development and show spontaneous SA accumulation, cell death, and defense-related markers later in development. In acd5 plants, cell death and defense-related responses are SA dependent but they do not confer disease resistance. Double mutants with acd5 and nonexpressor of PR1, in which SA signaling is partially blocked, show greatly attenuated cell death, indicating a role for NPR1 in controlling cell death. The hormone ethylene potentiates the effects of SA and is important for disease symptom development in Arabidopsis. Double mutants of acd5 and ethylene insensitive 2, in which ethylene signaling is blocked, show decreased cell death, supporting a role for ethylene in cell death control. We propose that acd5 plants mimic P. syringae-infected wild-type plants and that both SA and ethylene are normally involved in regulating cell death during some susceptible pathogen infections.

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Production of hydroxy fatty acids in the seeds of Arabidopsis thaliana

Biochemical Society Transactions ◽

10.1042/bst0280947 ◽

2000 ◽

Vol 28 (6) ◽

pp. 947-950 ◽

Cited By ~ 12

Author(s):

M. Smith ◽

H. Moon ◽

L. Kunst

Keyword(s):

Fatty Acids ◽

Arabidopsis Thaliana ◽

Seed Oil ◽

Desaturase Activity ◽

Hydroxy Fatty Acids ◽

Specific Expression ◽

Oleate Desaturase ◽

Arabidopsis Mutant ◽

Mutant Lines ◽

Seed Specific Expression

Seed-specific expression in Arabidopsis thaliana of oleate hydroxylase enzymes from castor bean and Lesquerella fendleri resulted in the accumulation of hydroxy fatty acids in the seed oil. By using various Arabidopsis mutant lines it was shown that the endoplasmic reticulum (ER) n–-3 desaturase (FAD3) and the FAE1 condensing enzyme are involved in the synthesis of polyunsaturated and very-long-chain hydroxy fatty acids, respectively. In Arabidopsis plants with an active ER Δ12-oleate desaturase the presence of hydroxy fatty acids corresponded to an increase in the levels of 18:1 and a decrease in 18:2 levels. Expression in yeast indicates that the castor hydroxylase also has a low level of desaturase activity.

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Improvement of arabidopsis mutant screens based on luciferase imagingin planta

Plant Molecular Biology Reporter ◽

10.1007/bf02673364 ◽

1996 ◽

Vol 14 (4) ◽

pp. 320-329 ◽

Cited By ~ 7

Author(s):

Baudouin Michelet ◽

Nam-Hai Chua

Keyword(s):

Arabidopsis Mutant

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Phosphoproteomic analysis of ethylene-regulated protein phosphorylation in etiolated seedlings of Arabidopsis mutant ein2 using two-dimensional separations coupled with a hybrid quadrupole time-of-flight mass spectrometer

PROTEOMICS ◽

10.1002/pmic.200800420 ◽

2009 ◽

Vol 9 (6) ◽

pp. 1646-1661 ◽

Cited By ~ 43

Author(s):

Hao Li ◽

Wai Shing Wong ◽

Lin Zhu ◽

Hong Wei Guo ◽

Joseph Ecker ◽

...

Keyword(s):

Protein Phosphorylation ◽

Mass Spectrometer ◽

Time Of Flight ◽

Two Dimensional ◽

Flight Mass Spectrometer ◽

Etiolated Seedlings ◽

Arabidopsis Mutant

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Transcriptome profiling and methyl homeostasis of an Arabidopsis mutant deficient in S-adenosylhomocysteine hydrolase1 (SAHH1)

Plant Molecular Biology ◽

10.1007/s11103-012-9914-1 ◽

2012 ◽

Vol 79 (4-5) ◽

pp. 315-331 ◽

Cited By ~ 14

Author(s):

Bo Ouyang ◽

Zhangjun Fei ◽

Je-Gun Joung ◽

Allan Kolenovsky ◽

Chushin Koh ◽

...

Keyword(s):

Transcriptome Profiling ◽

Arabidopsis Mutant

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Phosphorus and nitrogen interaction: loss of QC identity in response to P or N limitation is antecipated in pdr23 mutant

Brazilian Journal of Plant Physiology ◽

10.1590/s1677-04202011000300006 ◽

2011 ◽

Vol 23 (3) ◽

pp. 219-229 ◽

Cited By ~ 5

Author(s):

Cibele T. Costa ◽

Mércio L. Strieder ◽

Stephen Abel ◽

Carla A. Delatorre

Keyword(s):

Nucleic Acids ◽

Lateral Roots ◽

Adaptive Strategy ◽

Quiescent Center ◽

Wild Type ◽

Short Root ◽

Alternative Source ◽

Low Phosphorus ◽

Determinate Growth ◽

Arabidopsis Mutant

Changes in root architecture are an important adaptive strategy used by plants in response to limited nutrient availability to increase the odds of acquiring them. The quiescent center (QC) plays an important role by altering the meristem activity causing differentiation and therefore, inducing a determinate growth program. The arabidopsis mutant pdr23 presents primary short root in the presence of nitrate and is inefficient in the use of nucleic acids as a source of phosphorus. In this study the effect of the pdr23 mutation on the QC maintenance under low phosphorus (P) and/or nitrogen is evaluated. QC identity is maintained in wild-type in the absence of nitrate and/or phosphate if nucleic acids can be used as an alternative source of these nutrients, but not in pdr23. The mutant is not able to use nucleic acids efficiently for substitute Pi, determinate growth is observed, similar to wild-type in the total absence of P. In the absence of N pdr23 loses the expression of QC identity marker earlier than wild-type, indicating that not only the response to P is altered, but also to N. The data suggest that the mutation affects a gene involved either in the crosstalk between these nutrients or in a pathway shared by both nutrients limitation response. Moreover loss of QC identity is also observed in wild-type in the absence of N at longer limitation. Less drastic symptoms are observed in lateral roots of both genotypes.

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Altered metal distribution in the sr45-1 Arabidopsis mutant causes developmental defects

10.1101/2021.04.02.438214 ◽

2021 ◽

Author(s):

Steven Fanara ◽

Marie Schloesser ◽

Marc Hanikenne ◽

Patrick Motte

Keyword(s):

Stress Responses ◽

Molecular Mechanisms ◽

Phenylpropanoid Pathway ◽

Histochemical Staining ◽

Metal Distribution ◽

Iron Starvation ◽

Developmental Defects ◽

Developmental Abnormalities ◽

Arabidopsis Mutant ◽

Root Tissues

The plant SR (serine/arginine-rich) splicing factor SR45 plays important roles in several biological processes, such as splicing, DNA methylation, innate immunity, glucose regulation and ABA signaling. A homozygous Arabidopsis sr45-1 null mutant is viable, but exhibits diverse phenotypic alterations, including delayed root development, late flowering, shorter siliques with fewer seeds, narrower leaves and petals, and unusual numbers of floral organs. Here, we report that the sr45-1 mutant presents an unexpected constitutive iron deficiency phenotype characterized by altered metal distribution in the plant. RNA-Sequencing highlighted severe perturbations in metal homeostasis, phenylpropanoid pathway, oxidative stress responses, and reproductive development. Ionomic quantification and histochemical staining revealed strong iron accumulation in the sr45-1 root tissues accompanied by an iron starvation in aerial parts. We showed that some sr45-1 developmental abnormalities can be complemented by exogenous iron supply. Our findings provide new insight into the molecular mechanisms governing the phenotypes of the sr45-1 mutant.

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