EF-1a Is Associated with a Cytoskeletal Network Surrounding Protein Bodies in Maize Endosperm Cells

1996 ◽  
Vol 8 (11) ◽  
pp. 2003 ◽  
Author(s):  
Amy M. Clore ◽  
Joanne M. Dannenhoffer ◽  
Brian A. Larkins
Keyword(s):  
Protein Bodies ◽  
Maize Endosperm ◽  
Cytoskeletal Network

scholarly journals Synthesis and Deposition of Zein in Protein Bodies of Maize Endosperm

1978 ◽  
Vol 62 (2) ◽  
pp. 256-263 ◽  
Author(s):  
Brian A. Larkins ◽  
William J. Hurkman
Keyword(s):  
Protein Bodies ◽  
Maize Endosperm

Reduced Soluble Proteins Associated with Maize Endosperm Protein Bodies

1982 ◽  
Vol 33 (3) ◽  
pp. 439-448 ◽  
Author(s):  
ALESSANDRO VITALE ◽  
ELISA SMANIOTTO ◽  
RENATO LONGHI ◽  
ENNIO GALANTE
Keyword(s):  
Soluble Proteins ◽  
Protein Bodies ◽  
Maize Endosperm ◽  
Endosperm Protein

Three-Dimensional reconstruction of protein bodies in developing maize endosperm using immunocytochemical techniques

1994 ◽  
Vol 52 ◽  
pp. 330-331
Author(s):  
C. Lending ◽  
S. Spinelli
Keyword(s):  
Storage Proteins ◽  
Three Dimensional ◽  
Protein Body ◽  
Uranyl Acetate ◽  
Protein Bodies ◽  
Immunocytochemical Staining ◽  
Maize Endosperm ◽  
Dimensional Reconstruction ◽  
Immunocytochemical Techniques ◽  
Ultrathin Sections

The storage proteins of maize (Zea mays L.), like many other cereals, are a group of alcohol-soluble proteins classified as prolamines. In maize these proteins comprise four structurally distinct types and are termed zeins. Zein synthesis is initiated in developing maize endosperm and continues until the seed reaches maturity (between approximately 12 to 50 days after pollination). Zeins are synthesized by polysomes bound to the rough endoplasmic reticulum (RER) and they accumulate as insoluble aggregates called protein bodies. Our previous studies have shown that the zeins are deposited within protein bodies in a defined order in normal genotypes. However, the actual three-dimensional organization of the zeins within protein bodies and the interactions that occur between the various zeins is not known. Our goal is to understand protein body organization and the interactions that occur between the various proteins.The four types of zeins are identified by their molecular weight after separation by SDS-PAGE, and are designated α-, β-, γ- and 6-zeins. The β-, γ-, and δ-zeins are all sulfur-rich proteins, while the α-zeins contain only low amounts of cysteine and methionine. These proteins can be individually detected by immunocytochemical staining of ultrathin sections. Additionally, ultrathin sections poststained with lead citrate and uranyl acetate demonstrate that there are two distinct regions that correlate with the immunocytochemical staining patterns.

THE MECHANISM OF ZEIN SYNTHESIS AND DEPOSITION IN PROTEIN BODIES OF MAIZE ENDOSPERM

1979 ◽  
pp. 49-65 ◽  
Author(s):  
Brian A. Larkins ◽  
Nina L. Pearlmutter ◽  
William J. Hurkman
Keyword(s):  
Protein Bodies ◽  
Maize Endosperm

Changes in the Zein Composition of Protein Bodies during Maize Endosperm Development

1989 ◽  
Vol 1 (10) ◽  
pp. 1011 ◽  
Author(s):  
Craig R. Lending ◽  
Brian A. Larkins
Keyword(s):  
Endosperm Development ◽  
Protein Bodies ◽  
Maize Endosperm

scholarly journals Purification and translation of zein messenger RNA from maize endosperm protein bodies

1978 ◽  
Vol 75 (2) ◽  
pp. 696-700 ◽  
Author(s):  
B. Burr ◽  
F. A. Burr ◽  
I. Rubenstein ◽  
M. N. Simon
Keyword(s):  
Messenger Rna ◽  
Protein Bodies ◽  
Maize Endosperm ◽  
Endosperm Protein
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