Functional Domains of the Floral Regulator AGAMOUS: Characterization of the DNA Binding Domain and Analysis of Dominant Negative Mutations

1996 ◽  
Vol 8 (5) ◽  
pp. 831 ◽  
Author(s):  
Yukiko Mizukami ◽  
Hai Huang ◽  
Matthew Tudor ◽  
Yi Hu ◽  
Hong Ma
Keyword(s):  
Dna Binding ◽  
Binding Domain ◽  
Dominant Negative ◽  
Functional Domains ◽  
Dna Binding Domain ◽  
Dominant Negative Mutations

Dominant-negative mutations in the DNA-binding domain of STAT3 cause hyper-IgE syndrome

Nature ◽  
2007 ◽  
Vol 448 (7157) ◽  
pp. 1058-1062 ◽  
Author(s):  
Yoshiyuki Minegishi ◽  
Masako Saito ◽  
Shigeru Tsuchiya ◽  
Ikuya Tsuge ◽  
Hidetoshi Takada ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Domain ◽  
Dominant Negative ◽  
Dna Binding Domain ◽  
Hyper Ige Syndrome ◽  
Dominant Negative Mutations

scholarly journals Characterization of the Elk-1 ETS DNA-binding Domain

1995 ◽  
Vol 270 (11) ◽  
pp. 5805-5811 ◽  
Author(s):  
Paul Shore ◽  
Louise Bisset ◽  
Jeremy Lakey ◽  
Jonathan P. Waltho ◽  
Richard Virden ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Domain ◽  
Dna Binding Domain

Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1

1992 ◽  
Vol 12 (10) ◽  
pp. 4297-4304 ◽  
Author(s):  
D Ray ◽  
R Bosselut ◽  
J Ghysdael ◽  
M G Mattei ◽  
A Tavitian ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cell Lines ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
New Gene ◽  
Carboxy Terminal Region ◽  
Carboxy Terminal ◽  
Hematopoietic Cell Lines

We have cloned a human cDNA from a new gene, spi-B, on the basis of its homology with the DNA-binding domain of the Spi-1/PU.1 putative oncogene product. spi-B codes for a protein of 262 amino acids presenting 43% overall identity with Spi-1. Its highly basic carboxy-terminal region exhibits 34% sequence identity with the DNA-binding domain of the Ets-1 protein. We showed that the Spi-B protein is able to bind the purine-rich sequence (PU box) recognized by Spi-1/PU.1 and to activate transcription of a reporter plasmid containing PU boxes. Chromosome in situ hybridization allowed us to map spi-B to the 19q13.3-19q13.4 region of the human genome. spi-B, like spi-1, was found to be expressed in various murine and human hematopoietic cell lines except T lymphoid cell lines.

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