Inactivation of a Synechocystis sp Strain PCC 6803 Gene with Homology to Conserved Chloroplast Open Reading Frame 184 Increases the Photosystem II-to-Photosystem I Ratio

1995 ◽  
Vol 7 (5) ◽  
pp. 649
Author(s):  
Annegret Wilde ◽  
Heiko Hartel ◽  
Thomas Hubschmann ◽  
Paul Hoffmann ◽  
Sergey V. Shestakov ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Photosystem I ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Open Reading Frame ssr2016 is Required for Antimycin A-sensitive Photosystem I-driven Cyclic Electron Flow in the Cyanobacterium Synechocystis sp. PCC 6803

2005 ◽  
Vol 46 (8) ◽  
pp. 1433-1436 ◽  
Author(s):  
Nataliya Yeremenko ◽  
Robert Jeanjean ◽  
Peerada Prommeenate ◽  
Vladimir Krasikov ◽  
Peter J. Nixon ◽  
...  
Keyword(s):  
Photosystem I ◽  
Electron Flow ◽  
Cyclic Electron Flow ◽  
Open Reading Frame ◽  
Antimycin A ◽  
Reading Frame ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Type II Isopentenyl Diphosphate Isomerase from Synechocystis sp. Strain PCC 6803

2004 ◽  
Vol 186 (23) ◽  
pp. 8156-8158 ◽  
Author(s):  
Sam J. Barkley ◽  
Shrivallabh B. Desai ◽  
C. Dale Poulter
Keyword(s):  
Escherichia Coli ◽  
Open Reading Frame ◽  
Flavin Mononucleotide ◽  
Isopentenyl Diphosphate ◽  
Type Ii ◽  
Isopentenyl Diphosphate Isomerase ◽  
Reading Frame ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACT Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His6-tagged protein was produced in Escherichia coli and purified by Ni2+ chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg2+ for activity; K m IPP was 52 μM, and k cat IPP was 0.23 s−1.

scholarly journals The Synechocystis sp. PCC 6803 open reading frame slr0201 that is homologous to sdhC from Archaea codes for a [2Fe-2S] protein

2021 ◽  
Author(s):  
Fusheng Xiong ◽  
Russell LoBrutto ◽  
Wim F. J. Vermaas
Keyword(s):  
Sequence Similarity ◽  
Immunoblot Analysis ◽  
Open Reading Frame ◽  
Heterodisulfide Reductase ◽  
Redox Titration ◽  
High Sequence Similarity ◽  
Reading Frame ◽  
Midpoint Potential ◽  
Synechocystis Sp ◽  
Pcc 6803

A hypothetical protein encoded by Synechocystis sp. PCC 6803 open reading frame slr0201 shows high sequence similarity to the C subunit of a group of unusual succinate dehydrogenases found in some archaeal species. Slr0201 was originally annotated as HdrB, the B subunit of heterodisulfide reductase, but appears to be SdhC instead. This protein was overexpressed in E. coli by cloning the PCR-derived slr0201 open reading frame into a pET16b-based expression vector. The overproduced Slr0201 accumulated predominantly in inclusion bodies with an apparent molecular mass of 33 kDa. The protein contained at least one [2Fe-2S] cluster based on UV-visible absorbance and CD spectra and EPR spectroscopy, in conjunction with stoichiometric analysis of protein-bound iron and sulfur content. Redox titration showed a midpoint potential (Em) of + 17 mV at pH 7.0, which is consistent with Slr0201 serving a role in transferring electrons between succinate and plastoquinone. Slr0201 was also overproduced in Synechocystis sp. PCC 6803 by introducing an additional, His-tagged slr0201 into the Synechocystis genome replacing psbA3, creating the slr0201+-His overexpression strain. Immunoblot analysis shows that Slr0201 is membrane-associated in the wild type. However, in the Slr0201+-His strain, immunoreaction occurred in both the membrane and soluble fractions, possibly as a consequence of processing near the N-terminus. The results obtained with Slr0201 are discussed in the light of one of the cyanobacterial SdhB subunits, which shares redox commonalities with archaeal SdhB.

scholarly journals Complementation of a phycocyanin-bilin lyase from Synechocystis sp. PCC 6803 with a nucleomorph-encoded open reading frame from the cryptophyte Guillardia theta

2008 ◽  
Vol 8 (1) ◽  
pp. 56 ◽  
Author(s):  
Kathrin Bolte ◽  
Oliver Kawach ◽  
Julia Prechtl ◽  
Nicole Gruenheit ◽  
Julius Nyalwidhe ◽  
...  
Keyword(s):  
Open Reading Frame ◽  
Reading Frame ◽  
Guillardia Theta ◽  
Synechocystis Sp ◽  
Pcc 6803
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