A trans-Splicing Model for the Expression of the Tripartite nad5 Gene in Wheat and Maize Mitochondria

1991 ◽  
Vol 3 (12) ◽  
pp. 1363 ◽  
Author(s):  
Anete Pereira de Souza ◽  
Marie-France Jubier ◽  
Evelyne Delcher ◽  
Dominique Lancelin ◽  
Bernard Lejeune
Keyword(s):  
Trans Splicing ◽  
Nad5 Gene

scholarly journals A trans-splicing model for the expression of the tripartite nad5 gene in wheat and maize mitochondria.

1991 ◽  
Vol 3 (12) ◽  
pp. 1363-1378 ◽  
Author(s):  
A Pereira de Souza ◽  
M F Jubier ◽  
E Delcher ◽  
D Lancelin ◽  
B Lejeune
Keyword(s):  
Trans Splicing ◽  
Nad5 Gene

scholarly journals Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 125
Author(s):  
Piero Bonelli ◽  
Silvia Dei Giudici ◽  
Angela Peruzzu ◽  
Lorena Mura ◽  
Cinzia Santucciu ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Animal Species ◽  
Cost Effective ◽  
Sensu Stricto ◽  
Sybr Green ◽  
Hydatid Cysts ◽  
Genotype Identification ◽  
Host Selectivity ◽  
Nad5 Gene ◽  
Echinococcus Granulosus Sensu Lato

Echinococcus granulosus sensu lato (s.l.) is the causative agent of cystic echinococcosis in animals and humans. Different E. granulosuss.l. genotypes exhibit great diversity in their life cycle, host selectivity and pathogenicity. For this reason, the study of genetic variation within Echinococcus species is of importance for their epidemiological implication. We employed two SNP genotyping technologies to distinguish G1 and G3 E. granulosus sensu stricto (s.s.). genotypes. The genotypes of DNA samples (n = 28) extracted from hydatid cysts of different animal species were identified by amplification and sequencing of a fragment of the mitochondrial nad5 gene. Two SYBR green and three TaqMan real time PCR assays were developed for targeting of three nad5 informative positions (SNP758, 1123, and 1380) known to be able to discriminate G1 from G3. Genotyping by SYBR Green PCR based on cycle threshold (Ct) with melting temperature (Tm) analysis and performed on SNP1123 and SNP1380 failed to identify one DNA sample. TaqMan assays for SNP758, 1123 and 1380 effectively confirmed genotype identification obtained by Sanger sequencing. Our results demonstrated that the combination of the three Taqman assays developed in this study represents a valuable and cost effective tool alternative to DNA sequencing for E. granulosus s.s. genotyping.

scholarly journals Group II intron domain 5 facilitates a trans-splicing reaction.

1988 ◽  
Vol 8 (6) ◽  
pp. 2361-2366 ◽  
Author(s):  
K A Jarrell ◽  
R C Dietrich ◽  
P S Perlman
Keyword(s):  
Mitochondrial Dna ◽  
Group Ii Intron ◽  
Intron Sequence ◽  
Trans Splicing ◽  
Group Ii ◽  
Self Splicing ◽  
Yeast Mitochondrial Dna ◽  
Domain 4

A self-splicing group II intron of yeast mitochondrial DNA (aI5g) was divided within intron domain 4 to yield two RNAs that trans-spliced in vitro with associated trans-branching of excised intron fragments. Reformation of the domain 4 secondary structure was not necessary for the trans reaction, since domain 4 sequences were shown to be dispensable. Instead, the trans reaction depended on a previously unpredicted interaction between intron domain 5, the most highly conserved region of group II introns, and another region of the RNA. Domain 5 was shown to be essential for cleavage at the 5' splice site. It stimulated that cleavage when supplied as a trans-acting RNA containing only 42 nucleotides of intron sequence. The relevance of our findings to in vivo trans-splicing mechanisms is discussed.

Ion channel engineering using protein trans-splicing

2021 ◽  
Author(s):  
Debayan Sarkar ◽  
Hendrik Harms ◽  
Iacopo Galleano ◽  
Zeshan Pervez Sheikh ◽  
Stephan Alexander Pless
Keyword(s):  
Ion Channel ◽  
Trans Splicing ◽  
Channel Engineering

Repair of mutant CFTR mRNA by targeted RNA trans-splicing

2000 ◽  
Vol 118 (4) ◽  
pp. A185
Author(s):  
S.G. Mansfield ◽  
J. Kole ◽  
M. Puttaragu ◽  
C.C. Yang ◽  
M.A. Garcio-Blanco ◽  
...  
Keyword(s):  
Trans Splicing
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