Localization of Elements Important for the Wound-Inducible Expression of a Chimeric Potato Proteinase Inhibitor II-CAT Gene in Transgenic Tobacco Plants

1990 ◽  
Vol 2 (1) ◽  
pp. 61 ◽  
Author(s):  
Michael Keil ◽  
Jose Sanchez-Serrano ◽  
Jeff Schell ◽  
Lothar Willmitzer
Keyword(s):  
Transgenic Tobacco ◽  
Proteinase Inhibitor ◽  
Inducible Expression ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Proteinase Inhibitor Ii ◽  
Cat Gene ◽  
Potato Proteinase Inhibitor Ii

scholarly journals Wound-induced expression of a potato proteinase inhibitor II gene in transgenic tobacco plants

1987 ◽  
Vol 6 (2) ◽  
pp. 303-306 ◽  
Author(s):  
Jose J. Sanchez-Serrano ◽  
Michael Keil ◽  
Aileen O'Connor ◽  
Jeff Schell ◽  
Lothar Willmitzer
Keyword(s):  
Transgenic Tobacco ◽  
Proteinase Inhibitor ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Induced Expression ◽  
Proteinase Inhibitor Ii ◽  
Potato Proteinase Inhibitor Ii

scholarly journals Signal Peptide of Potato PinII Enhances the Expression of Cry1Ac in Transgenic Tobacco

2004 ◽  
Vol 36 (8) ◽  
pp. 553-558 ◽  
Author(s):  
Yun-Jun Liu ◽  
Yuan Yuan ◽  
Jun Zheng ◽  
Ya-Zhong Tao ◽  
Zhi-Gang Dong ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Signal Peptide ◽  
Proteinase Inhibitor ◽  
Peptide Sequence ◽  
Fluorescent Detection ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Signal Peptide Sequence ◽  
Proteinase Inhibitor Ii ◽  
Cry1ac Protein

Abstract The modified Cry1Ac was expressed in transgenic tobacco plants. To allow secretion of the Cry1Ac protein into the intercellular space, the signal peptide sequence of potato proteinase inhibitor II (pinII) was N-terminally fused to the Cry1Ac encoding region. Expression of Cry1Ac in transgenic tobacco plants was assayed with ELISA. The results showed that pinII signal peptide sequence enhanced the expression of Cry1Ac protein and led to the secretion of the Cry1Ac protein in transgenic tobacco plants. GFP gene was also fused to the signal peptide sequence and transformed to tobacco. The results of fluorescent detection showed that GFP had localized in the apoplast of transgenic plants.

Expression of a cysteine proteinase inhibitor (oryzacystatin-I) in transgenic tobacco plants

1993 ◽  
Vol 21 (4) ◽  
pp. 655-663 ◽  
Author(s):  
Sameer A. Masoud ◽  
Lowell B. Johnson ◽  
Frank F. White ◽  
Gerald R. Reeck
Keyword(s):  
Transgenic Tobacco ◽  
Proteinase Inhibitor ◽  
Cysteine Proteinase ◽  
Cysteine Proteinase Inhibitor ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants
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