Characterization of Fusarium graminearum from Acacia and Eucalyptus Using [beta]-Tubulin and Histone Gene Sequences

Mycologia ◽  
2001 ◽  
Vol 93 (4) ◽  
pp. 704 ◽  
Author(s):  
Jolanda Roux ◽  
Emma T. Steenkamp ◽  
Wally F. O. Marasas ◽  
Michael J. Wingfield ◽  
Brenda D. Wingfield
Keyword(s):  
Fusarium Graminearum ◽  
Histone Gene ◽  
Gene Sequences ◽  
Beta Tubulin

scholarly journals GENETIC CHARACTERIZATION OF THE REGION OF THE DROSOPHILA GENOME KNOWN TO INCLUDE THE HISTONE STRUCTURAL GENE SEQUENCES

Genetics ◽  
1981 ◽  
Vol 98 (3) ◽  
pp. 505-527
Author(s):  
Jacqueline G Siegel
Keyword(s):  
Structural Gene ◽  
Genetic Study ◽  
Genetic Characterization ◽  
Histone Gene ◽  
Drosophila Genome ◽  
Gene Sequences ◽  
Lethal Mutations ◽  
Gene Functions ◽  
Complementation Groups

ABSTRACT This report describes a genetic study of salivary map region 39DE of the Drosophila genome, which is known to include the histone gene sequences (Pardue 1975; Lifton et al. 1977). Small deficiences extending proximally into 39DE were constructed by the segmental aneuploid method of Lindsley et al. (1972). The translocational deficiencies obtained in this manner were γ-irradiated to remove the Y translocational arms. One of these newly reconstituted deficiencies was then used to screen 10,000 γ-irradiated second chromosomes for lethal mutations. The 32 lethals recovered from the screen were tested against several deficiencies and markers, crossed inter se and categorized according to their genetic properties. From these data, a preliminary complementation map was constructed of salivary region 39A-39F. The salivary map positions of certain of the complementation groups suggest that the mutants in these groups may affect histone gene functions.

Molecular genetic characterization of rabies virus glycoprotein gene sequences from rabid dogs in Bangkok and neighboring provinces in Thailand, 2013–2014

2016 ◽  
Vol 161 (5) ◽  
pp. 1261-1271 ◽  
Author(s):  
Surachet Benjathummarak ◽  
Chanon Fa-ngoen ◽  
Chonlatip Pipattanaboon ◽  
Khwanchit Boonha ◽  
Pongrama Ramasoota ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Genetic Characterization ◽  
Molecular Genetic ◽  
Gene Sequences ◽  
Rabies Virus Glycoprotein ◽  
Virus Glycoprotein ◽  
Glycoprotein Gene ◽  
Molecular Genetic Characterization

scholarly journals Molecular Characterization of anEndozoicomonas-Like Organism Causing Infection in the King Scallop (Pecten maximusL.)

2017 ◽  
Vol 84 (3) ◽  
Author(s):  
Irene Cano ◽  
Ronny van Aerle ◽  
Stuart Ross ◽  
David W. Verner-Jeffreys ◽  
Richard K. Paley ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Mass Mortality ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
The 16S Rrna Gene

ABSTRACTOne of the fastest growing fisheries in the UK is the king scallop (Pecten maximusL.), also currently rated as the second most valuable fishery. Mass mortality events in scallops have been reported worldwide, often with the causative agent(s) remaining uncharacterized. In May 2013 and 2014, two mass mortality events affecting king scallops were recorded in the Lyme Bay marine protected area (MPA) in Southwest England. Histopathological examination showed gill epithelial tissues infected with intracellular microcolonies (IMCs) of bacteria resemblingRickettsia-like organisms (RLOs), often with bacteria released in vascular spaces. Large colonies were associated with cellular and tissue disruption of the gills. Ultrastructural examination confirmed the intracellular location of these organisms in affected epithelial cells. The 16S rRNA gene sequences of the putative IMCs obtained from infected king scallop gill samples, collected from both mortality events, were identical and had a 99.4% identity to 16S rRNA gene sequences obtained from “CandidatusEndonucleobacter bathymodioli” and 95% withEndozoicomonasspecies.In situhybridization assays using 16S rRNA gene probes confirmed the presence of the sequenced IMC gene in the gill tissues. Additional DNA sequences of the bacterium were obtained using high-throughput (Illumina) sequencing, and bioinformatic analysis identified over 1,000 genes with high similarity to protein sequences fromEndozoicomonasspp. (ranging from 77 to 87% identity). Specific PCR assays were developed and applied to screen for the presence of IMC 16S rRNA gene sequences in king scallop gill tissues collected at the Lyme Bay MPA during 2015 and 2016. There was 100% prevalence of the IMCs in these gill tissues, and the 16S rRNA gene sequences identified were identical to the sequence found during the previous mortality event.IMPORTANCEMolluscan mass mortalities associated with IMCs have been reported worldwide for many years; however, apart from histological and ultrastructural characterization, characterization of the etiological agents is limited. In the present work, we provide detailed molecular characterization of anEndozoicomonas-like organism (ELO) associated with an important commercial scallop species.

Isolation and characterization of eight tumor necrosis factor-induced gene sequences from human fibroblasts

1990 ◽  
Vol 10 (5) ◽  
pp. 1982-1988
Author(s):  
T H Lee ◽  
G W Lee ◽  
E B Ziff ◽  
J Vilcek
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Human Fibroblasts ◽  
Gene Sequences ◽  
Cdna Sequences ◽  
Isolation And Characterization ◽  
Polyadenylated Rna ◽  
Second Messenger Pathways ◽  
Necrosis Factor

A lambda cDNA library was prepared from polyadenylated RNA isolated from quiescent human diploid FS-4 fibroblasts stimulated with tumor necrosis factor for 3 h. Differential screening was used to isolate cDNA sequences that are stimulated by tumor necrosis factor. Eight distinct tumor necrosis factor-stimulated gene sequences (designated TSG-1, -6, -8, -12, -14, -21, -27, and -37) were partially sequenced and compared with known sequences from GenBank. TSG-1 was identical to the gene for interleukin-8. TSG-8 corresponded to the gene for monocyte chemotactic and activating factor. TSG-21 and -27 were identical to the genes for collagenase and stromelysin, respectively. The other four sequences showed no homologies with known genes. Patterns of induction of mRNAs corresponding to the eight cloned cDNAs by various cytokines, growth factors, and activators of second messenger pathways were analyzed in FS-4 cells.

scholarly journals Prevalence and Genetic Characterization of Two Mitochondrial Gene Sequences of Strobilocercus Fasciolaris in the Livers of Brown Rats (Rattus norvegicus) in Heilongjiang Province in Northeastern China

2020 ◽  
Vol 10 ◽  
Author(s):  
Fengnian Zhao ◽  
Yun Zhou ◽  
Yanchen Wu ◽  
Kexin Zhou ◽  
Aiqin Liu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Intraspecific Variation ◽  
Rattus Norvegicus ◽  
Genetic Characterization ◽  
Northeastern China ◽  
Amino Acid Sequences ◽  
Morphological Observation ◽  
Heilongjiang Province ◽  
Gene Sequences

Rodents constitute the largest and most successful group of mammals worldwide. Brown rats (Rattus norvegicus) are one of the most common rodent species, and they serve as intermediate hosts of Hydatigera taeniaeformis. Although there have been a few studies reporting on the presence of the larval form of H. taeniaeformis (strobilocercus fasciolaris) in brown rats worldwide, little information is available on the genetic characterization of this parasite, with no molecular data from China. Therefore, from April 2014 to March 2016, this study was carried out to understand the prevalence and genetic characters of strobilocercus fasciolaris in brown rats captured in Heilongjiang Province in northeastern China. The livers of brown rats were collected and examined for the presence of cysts. Each cyst was identified based on morphological observation: the larvae with the naked eye and the scolexes under a microscope. The results were confirmed by polymerase chain reaction (PCR) and sequencing of the cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4) genes. At the investigated sites, 11.8% (13/110) of the brown rats were infected with strobilocercus fasciolaris. Based on sequence analysis, there were 10 and six haplotypes regarding the cox1 and the nad4 loci, with 24 and 42 polymorphic sites, respectively (degree of intraspecific variation: 0.3%–4.4% and 0.6%–4.7%, respectively). Twelve nucleotide sequences (six of the 10 at the cox1 locus and all six at the nad4 locus) have not previously been described. Base differences in three of the six novel cox1 gene sequences and five of the six novel nad4 gene sequences caused amino acid changes. Phylogenetic analyses of the cox1 and nad4 gene sequences based on neighbor-joining and Bayesian inference trees indicated that all the strobilocercus fasciolaris isolates belonged to Hydatigera taeniaeformis sensu stricto (s.s.). This is the first report on the genetic characterization of strobilocercus fasciolaris in brown rats in China. The findings of novel cox1 and nad4 nucleotide and amino acid sequences may reflect the region-specific genetic characterization of the parasite. The data will be useful to explore the biological and epidemiological significance of the intraspecific variation within H. taeniaeformis s.s.

scholarly journals Characterization of the 55-kb mouse histone gene cluster on chromosome 3.

1996 ◽  
Vol 6 (8) ◽  
pp. 702-714 ◽  
Author(s):  
Z F Wang ◽  
R Tisovec ◽  
R W Debry ◽  
M R Frey ◽  
A G Matera ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Histone Gene ◽  
Chromosome 3

scholarly journals Molecular characterization of a novel mycovirus of the family Tymoviridae isolated from the plant pathogenic fungus Fusarium graminearum

Virology ◽  
2016 ◽  
Vol 489 ◽  
pp. 86-94 ◽  
Author(s):  
Pengfei Li ◽  
Yanhong Lin ◽  
Hailong Zhang ◽  
Shuangchao Wang ◽  
Dewen Qiu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Fusarium Graminearum ◽  
Pathogenic Fungus ◽  
Plant Pathogenic Fungus ◽  
The Family
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