Selective Isolation from Soil and Separation in Vitro of P and Q Strains of Trichoderma virens with Differential Media

Mycologia ◽  
1999 ◽  
Vol 91 (6) ◽  
pp. 930 ◽  
Author(s):  
Charles R. Howell
Keyword(s):  
Trichoderma Virens ◽  
Selective Isolation

scholarly journals An in vitro study of the antifungal activity of Trichoderma virens 7b and a profile of its non-polar antifungal components released against Ganoderma boninense

2016 ◽  
Vol 54 (11) ◽  
pp. 732-744 ◽  
Author(s):  
Lee Pei Lee Angel ◽  
Mohd Termizi Yusof ◽  
Intan Safinar Ismail ◽  
Bonnie Tay Yen Ping ◽  
Intan Nur Ainni Mohamed Azni ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Trichoderma Virens ◽  
Ganoderma Boninense

Selective isolation of in vitro phase II conjugates using a lipophilic anionic exchange solid phase extraction method

2008 ◽  
Vol 863 (2) ◽  
pp. 242-248 ◽  
Author(s):  
Sébastien Gagné ◽  
Julie Laterreur ◽  
Louiza Mahrouche ◽  
Dan Sørensen ◽  
Jacques Yves Gauthier ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Phase Ii ◽  
Extraction Method ◽  
Solid Phase ◽  
Phase Extraction ◽  
Selective Isolation ◽  
Anionic Exchange

scholarly journals Bio-efficacy of Trichoderma species against Pigeonpea wilt pathogen

2017 ◽  
Vol 9 (4) ◽  
pp. 2327-2331 ◽  
Author(s):  
Balkishan Chaudhary ◽  
Sanjeev Kumar ◽  
Shiva Kant Kushwaha
Keyword(s):  
Volatile Compounds ◽  
Trichoderma Harzianum ◽  
Mycelial Growth ◽  
Biocontrol Agent ◽  
Trichoderma Viride ◽  
Control Agent ◽  
Trichoderma Virens ◽  
Trichoderma Species ◽  
Fusarium Udum

Three biocontrol agent viz., Trichoderma viride, Trichoderma virens and Trichoderma harzianum were evaluated to test the antagonism against Fusarium udum under in vitro conditions. All the three biocontrol agents have the potential of parasitizing the growth of Fusarium udum in vitro. The rate of parasitism was found fastest in T. viride (61.12% over growth in 96 hrs) than T. virens and T. harzianum. The volatile compounds from Trichoderma viride suppressed the mycelial growth of Fusarium udum by 43.13% and found effective when compared to Tricho-derma virens and Trichoderma harzianum. Non-volatile compounds or culture filtrate from Trichoderma virens at 15% concentration shows complete mycelial inhibition of the test fungi. The antagonist T. virens was chosen to be the most promising bio-control agent for F. udum.

Effects of Trichoderma Species on the Growth of Fusarium Verticillioides

2021 ◽  
Vol 50 (2) ◽  
pp. 423-425
Author(s):  
Ramesh Kumar ◽  
Sanjeev Kumar ◽  
Balkishan Chaudhary
Keyword(s):  
Trichoderma Harzianum ◽  
Mycelial Growth ◽  
Fusarium Verticillioides ◽  
Trichoderma Viride ◽  
Maximum Growth ◽  
Culture Technique ◽  
Dual Culture ◽  
Trichoderma Virens ◽  
Trichoderma Species

Six biocontrol treatments viz., Trichoderma viride, Trichoderma virens, Trichoderma harzianum, T. harzianum + T. viride, T. harzianum + T. virens and T. viride + T. virens were evaluated to test the antagonism against Fusarium verticillioides under in vitro conditions. The maximum growth inhibition (90.6%) was recorded in consortium of T. harzianum + T. viride in dual culture technique . The volatile and non volatile compounds from the consortium of T. harzianum + T. viride also found best and suppressed the mycelial growth of F. verticillioides to the tune of 83.90 and 84.61 %, respectively. Bangladesh J. Bot. 50(2): 423-425, 2021 (June)

scholarly journals Fungos antagonistas a Sclerotinia sclerotiorum em pepineiro cultivado em estufa

2005 ◽  
Vol 30 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Luciana Z. Ethur ◽  
Elena Blume ◽  
Marlove Muniz ◽  
Antonio Carlos F. da Silva ◽  
Daniela R. Stefanelo ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Sclerotinia Sclerotiorum ◽  
Trichoderma Virens

O mofo branco causado por Sclerotinia sclerotiorum pode inviabilizar o cultivo de olerícolas em ambiente protegido. Para elaborar-se um programa de controle biológico desse patógeno, necessita-se de antagonistas adequados. Este trabalho objetivou selecionar antagonistas fúngicos eficazes no controle de S. sclerotiorum em pepineiro (Cucumis sativus) cultivado em estufa, bem como, analisar a interferência dos antagonistas no crescimento vegetal. Foram utilizados um isolado de S. sclerotiorum obtido de pepineiro e 112 isolados fúngicos de quatro gêneros: Trichoderma, Fusarium, Penicillium e Aspergillus. Em experimento in vitro, foi utilizada a técnica do papel celofane e selecionados oito isolados de Trichoderma virens, os quais promoveram maior inibição no crescimento do patógeno (94 a 100%). Dois experimentos in vivo foram desenvolvidos em estufa utilizando-se substrato autoclavado e não autoclavado, em copos plásticos, e substrato não autoclavado, em sacos plásticos; o substrato foi infestado com S. sclerotiorum e foram utilizados oito isolados de T. virens como antagonistas. Todos os isolados testados controlaram o tombamento de mudas, mas o efeito sobre o crescimento vegetal variou de acordo com os isolados e o tratamento do substrato.

scholarly journals Evaluation of Antifusarium and Auxin Production of Trichoderma virens InaCC F1030 Isolated from Rhizosphere of Banana

2020 ◽  
Vol 2 (1) ◽  
pp. 31-39
Author(s):  
Toga Pangihotan Napitupulu
Keyword(s):  
Culture Collection ◽  
Trichoderma Virens ◽  
Plant Host ◽  
Trichoderma Species ◽  
Auxin Production ◽  
Volatile Organic ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Banana rhizosphere harbors a unique diversity of microbes including fungi that play critical roles in the growth of the plant host as well as might be important for biologically controlling the fungal soil-borne pathogens particularly Fusarium oxysporum f.sp. cubense (Foc), the causing agent of devastating Panama wilt. Among other fungi, we have succeeded to isolate a Trichoderma species from rhizosphere of healthy banana. Molecular identification revealed the isolate as Trichoderma virens InaCC F1030 (being collection of Indonesian Culture Collection or InaCC). Therefore, the aim of this study was to investigate the biological control of our isolate against Foc as well as plant growth promoting ability through its ability to produce auxin (indole-3-acetic acid/IAA). Two approaches were employed to evaluate the antagonism of our isolate against Foc, through direct confrontation test and volatile organic compounds (VOCs) producing. We found that our isolate was considered as antagonistic to the Foc, but not highly antagonistic according to direct confrontation assay. It was also revealed that our isolate produces the VOCs that inhibited around 50% of the mycelial growth of the test pathogen after six to seven days of exposure. Our isolate was able to produce the IAA in axenic submerged fermentation condition particularly in the presence of the precursor L-tryptophan. IAA production ability as well as the mycelial biomass of fungus were increased approximately 17% and 120% respectively as the effect of supplementation of 0.1% of L-tryptophan. These in vitro bioassays lead us to conclude that somehow our isolate T. virens InaCC F1030 has potency to be utilized as biocontrol and biofertilizer agent.

scholarly journals Pengaruh Dosis dan Frekuensi Aplikasi Biofungisida Trichoderma terhadap Infeksi Rigidoporus microporus pada Benih Karet

2018 ◽  
Vol 5 (2) ◽  
pp. 49
Author(s):  
Widi Amaria ◽  
Rita Harni ◽  
Edi Wardiana
Keyword(s):  
Incubation Period ◽  
Block Design ◽  
Trichoderma Virens ◽  
Population Number ◽  
Randomized Block Design ◽  
Application Frequency ◽  
Attack Intensity ◽  
Screen House

<em>Biological agents </em>Trichoderma virens<em> and </em>T. amazonicum<em> have been developed and examined for their effectiveness through in vitro and in vivo approaches against </em>Rigidoporus microporus<em>, the cause of white root disease (WRD) in rubber. The effectiveness of these bio-agents can be determined by testing the dosage and frequency of </em>Trichoderma <em>spp. biofungicide application. The research aimed to investigate the effective dose and application frequency of </em>Trichoderma<em> spp. biofungicide on </em>R. microporus<em> infection in rubber seedling. The experiment was conducted in laboratory and screen house of Indonesian</em><em> Industrial and Beverage Crops Research Institute (IIBCRI), Sukabumi, from June to December 2014. A randomized block design was used with 14 treatments and 3 replications, i.e biofungicide combination (</em>T. virens<em> and </em>T. amazonicum<em>), dosage (25, 50, and 75 g), application frequencies (1 and 2 times application), and two controls (positive and negative). Rubber seedlings used were propellegitiem seeds of GT1 clone planted in polybags. </em>Trichoderma<em> spp. was multiplied using fermentation method in liquid medium, whereas biofungicide was formulated using talc as carrier. Observed variables including </em>Trichoderma<em> spp. population number, incubation period, attack intensity, and WRD attack suppression. The results showed that </em>T. virens<em> and </em>T. amazonicum<em> biofungicides with 50 g/plant dose at one application was the most effective and efficient in suppressing </em>R. microporus<em> development on rubber seedlings. The type, dosage, and frequencies of application increased </em>Trichoderma<em> spp. population in soil, prolonged the pathogen’s incubation period, decreased WRD attack intensity, and suppress the attack of WRD disease.</em>

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