Potential for Outcrossing in an Apparently Asexual Population of Fusarium circinatum, the Causal Agent of Pitch Canker Disease

Mycologia ◽  
2000 ◽  
Vol 92 (6) ◽  
pp. 1085 ◽  
Author(s):  
Karen Wikler ◽  
Thomas R. Gordon ◽  
Sharon L. Clark ◽  
Michael J. Wingfield ◽  
Henriette Britz
Keyword(s):  
Causal Agent ◽  
Pitch Canker ◽  
Fusarium Circinatum ◽  
Asexual Population ◽  
Canker Disease

Potential for outcrossing in an apparently asexual population ofFusarium circinatum, the causal agent of pitch canker disease

Mycologia ◽  
2000 ◽  
Vol 92 (6) ◽  
pp. 1085-1090 ◽  
Author(s):  
Karen Wikler ◽  
Thomas R. Gordon ◽  
Sharon L. Clark ◽  
Michael J. Wingfield ◽  
Henriette Britz
Keyword(s):  
Causal Agent ◽  
Pitch Canker ◽  
Asexual Population ◽  
Canker Disease

SEMIOCHEMICALS FROM THREE SPECIES OF PITYOPHTHORUS (COLEOPTERA: SCOLYTIDAE): IDENTIFICATION AND FIELD RESPONSE

2000 ◽  
Vol 132 (6) ◽  
pp. 889-906 ◽  
Author(s):  
Paul L. Dallara ◽  
Steven J. Seybold ◽  
Holger Meyer ◽  
Till Tolasch ◽  
Wittko Francke ◽  
...  
Keyword(s):  
Aggregation Pheromone ◽  
Pinus Radiata ◽  
Causal Agent ◽  
Pheromone Component ◽  
Pitch Canker ◽  
Fusarium Circinatum ◽  
Canker Disease ◽  
Porapak Q ◽  
Field Response ◽  
Opposite Sex

AbstractAnalyses of pentane extracts of frass, whole beetles, and volatiles trapped on Porapak-Q from Pityophthorus Eichhoff spp. fed on Pinus radiata D. Don demonstrated that (E)-pityol [2-(1-hydroxy-1-methylethyl)-5-methyltetrahydrofuran] was produced by male Pityophthorus carmeli Swaine, female Pityophthorus nitidulus (Mannerheim), and female Pityophthorus setosus Blackman. (E)-(−)-Conophthorin) [(5S,7S)-(−)-7-methyl-1,6-dioxaspiro[4.5]decane] was produced by male P. carmeli and male P. nitidulus. Only the (2R,5S)-(+) stereoisomer of (E)-pityol was produced by male P. carmeli and female P. setosus. In field bioassays in central coastal California, P. setosus was attracted to (E)-(+)-pityol, whereas P. carmeli responded only to a combination of (E)-(−)-conophthorin and (E)-(+)-pityol. Male P. setosus and female P. carmeli responded to these treatments with larger numbers than opposite-sex conspecifics. (E)-(−)-Conophthorin alone did not attract species of Pityophthorus but significantly reduced catches of P. setosus to (E)-(+)-pityol. Lasconotus pertenuis Casey (Coleoptera: Colydiidae) and Ips mexicanus (Hopkins) (Coleoptera: Scolytidae) were attracted to a combination of (E)-(−)-conophthorin and (E)-(+)-pityol, and showed a trend for attraction to all (E)-(−)-conophthorin-containing treatments. (E)-(−)-Pityol was neither attractive nor interruptive for any taxon. (E)-(+)-Pityol is shown to be an aggregation pheromone component for P. carmeli and P. setosus. (E)-(−)-Conophthorin functions as a pheromone component for P. carmeli and may also function as a synomone that decreases competition of P. carmeli and P. nitidulus with P. setosus and as a kairomone for L. pertenuis. These semiochemicals have been useful in studying relationships among twig insects and the pathogen Fusarium circinatum (Nirenberg and O’Donnell), causal agent of pitch canker disease in P. radiata.

scholarly journals Outbreak of Pitch Canker Caused by Fusarium circinatum on Pinus spp. in Northern Spain

Plant Disease ◽  
2005 ◽  
Vol 89 (9) ◽  
pp. 1015-1015 ◽  
Author(s):  
E. Landeras ◽  
P. García ◽  
Y. Fernández ◽  
M. Braña ◽  
O. Fernández-Alonso ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Morphological Identification ◽  
Pitch Canker ◽  
Fusarium Circinatum ◽  
Northern Spain ◽  
Centraalbureau Voor ◽  
Canker Disease ◽  
Intergenic Spacer Region ◽  
Appl Environ ◽  
Pinus Spp

During the winter of 2003-2004, dieback symptoms were observed on Pinus radiata and P. pinaster in pine nurseries in Asturias (northern Spain). Small groups of affected seedlings appeared randomly distributed throughout the nurseries. The seedlings died rapidly, showing basal needle dieback, stem lesions, resin exudations, and wilting. Isolations from infected material onto potato dextrose agar (PDA) supplemented with 0.5 mg/ml of streptomycin sulfate and Komada's medium consistently yielded Fusarium sp. cultures. The isolates were transferred to PDA and Spezieller Nährstoffarmer agar and incubated at 25°C for 10 days with a 12-h photoperiod. The cultures were identified as Fusarium circinatum Nirenberg & O'Donnell (= Fusarium subglutinans Wollenweb. & Reinking), causal agent of pitch canker disease, on basis of the presence of polyphialides and characteristic sterile, coiled, hyphae (2). To further confirm their identity, a polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) based on histone H3 gene sequences (4) and a test based on the F. circinatum-specific primers, CIRC1A-CIRC4A, which amplifies a 360-bp DNA fragment of the intergenic spacer region of the nuclear ribosomal operon (3), were used. Results obtained with both techniques confirmed the morphological identification of the cultures. A representative culture has been placed in the Centraalbureau voor Schimmelcultures (CBS 117843). The pathogen was isolated only from seedlings of P. radiata and P. pinaster. Other species such as P. nigra, P. sylvestris, and Pseudotsuga menziesii, which were also grown in these nurseries, did not show symptoms. Pathogenicity was confirmed by inoculating 6- to 9-month-old P. radiata and P. pinaster seedlings. Small strips of bark (10 × 1 mm) were cut from the stems and similar sized pieces of PDA colonized by F. circinatum were placed in contact with the open wounds and covered with parafilm. Basal needle dieback was observed 10 days after inoculation that resulted in wilting of the seedlings. F. circinatum was reisolated from the affected stems fulfilling Koch's postulates. Later in the year, symptoms of pitch canker were also observed on 20-year-old P. radiata in one forest plantation in Cantabria (northern Spain). Infected branches and shoots of the trees exudated abundant resin, resulting in resinous cankers. The needles, distal to branch tip infections, wilt, fade to yellow then red, and fall from the tree. Affected trees showed noticeable crown dieback. The isolations from the cankers also yielded F. circinatum cultures that were identified as described above. Although a nonrefereed report appeared in 1998 (1), to our knowledge, this is the first report of F. circinatum on P. radiata and P. pinaster in Spain and in Europe. References: (1) L. D. Dwinell et al. Int. Congr. Plant Pathol. 7th. 3:9, 1998. (2) H. I. Nirenberg and K. O'Donnell. Mycologia 90:434, 1998. (3) W. Schweigkofler et al. Appl. Environ. Microbiol. 70:3512, 2004. (4) E. T. Steenkamp et al. Appl. Environ. Microbiol. 65:3401, 1999.

Association levels between Pityophthorus pubescens and Fusarium circinatum in pitch canker disease affected plantations in northern Spain

2016 ◽  
Vol 36 (1) ◽  
pp. 43-54 ◽  
Author(s):  
Diana Bezos ◽  
Pablo Martínez-Álvarez ◽  
Julio J. Diez ◽  
Mercedes Fernández
Keyword(s):  
Pitch Canker ◽  
Fusarium Circinatum ◽  
Northern Spain ◽  
Canker Disease

scholarly journals First Report of Fusarium circinatum, Causal Agent of Pitch Canker Disease, from the Roots of Mature Aleppo Pines in California

2007 ◽  
Vol 8 (1) ◽  
pp. 55 ◽  
Author(s):  
M. Garbelotto ◽  
W. Schweigkofler ◽  
D. Shaw
Keyword(s):  
San Diego ◽  
Significant Source ◽  
Rapid Decline ◽  
Pitch Canker ◽  
Fusarium Circinatum ◽  
Mature Trees ◽  
Amusement Park ◽  
Canker Disease ◽  
First Report ◽  
Parking Lot

A rapid decline of Aleppo pines was observed in the parking lot of the Legoland amusement park in San Diego Co., CA. Although Aleppo pine is a known host for Fusarium circinatum, this is the first report describing resin-soaked sapwood lesions in the roots of mature trees. Previous reports on root infections regarded seedlings. The infestation in this location may have begun with infected roots in planted saplings. The persistent infestation at Legoland represents a significant source of inoculum for this pathogen exotic to California. Accepted for publication 7 June 2006. Published 19 February 2007.

An initial assessment of genetic relationships among populations of Fusarium circinatum in different parts of the world

2000 ◽  
Vol 78 (6) ◽  
pp. 709-717 ◽  
Author(s):  
Karen Wikler ◽  
Thomas R Gordon
Keyword(s):  
Genetic Relationships ◽  
Initial Assessment ◽  
Multilocus Genotype ◽  
Pitch Canker ◽  
Fusarium Circinatum ◽  
Canker Disease ◽  
Vegetative Compatibility Groups ◽  
Tree Disease ◽  
Genotype Frequencies ◽  
Pinus Spp

Fusarium circinatum Nirenberg & O'Donnell, the fungus responsible for pitch canker disease, is a destructive pathogen of Pinus spp. Pitch canker was first described in 1946 in the southeastern United States, and since 1987 has been reported in numerous other locations including California, Mexico, Japan, and South Africa. To make a preliminary assessment of relationships between populations of F. circinatum in these different locations, we compared allele and genotype frequencies based on eight polymorphic regions of DNA from 76 isolates of the fungus. Patterns of relatedness indicate that the California and Japanese populations of the fungus share lineages with the southeastern U.S.A. population. Genetic diversity is highest in Mexico, implicating it as the center of origin for the fungus. The association of multiple vegetative compatibility groups with a common multilocus genotype suggests that vegetative compatible group diversity may be generated by mutation, rather than through recombination resulting from sexual reproduction.Key words: genomic subtraction, tree disease, genetic distance.

scholarly journals First report and characterization of Fusarium circinatum, the causal agent of pitch canker in Brazil

2014 ◽  
Vol 39 (3) ◽  
pp. 210-216 ◽  
Author(s):  
Ludwig H. Pfenning ◽  
Sarah da Silva Costa ◽  
Maruzanete Pereira de Melo ◽  
Hélcio Costa ◽  
José Aires Ventura ◽  
...  
Keyword(s):  
Causal Agent ◽  
Pitch Canker ◽  
Fusarium Circinatum ◽  
First Report
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