Identification of Arbuscular Mycorrhizal Fungi from Artemisia californica Using the Polymerase Chain Reaction

Mycologia ◽  
1997 ◽  
Vol 89 (4) ◽  
pp. 547 ◽  
Author(s):  
S. K. Harney ◽  
F. S. Edwards ◽  
M. F. Allen
Keyword(s):  
Polymerase Chain Reaction ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Chain Reaction ◽  
Artemisia Californica ◽  
Polymerase Chain

scholarly journals Interactions with mycorrhizal fungi in two closely related hybridizing orchid species

2014 ◽  
Vol 73 (1) ◽  
pp. 322-333 ◽  
Author(s):  
Alessia Luca ◽  
Francesca Bellusci ◽  
Giuseppe Pellegrino
Keyword(s):  
Polymerase Chain Reaction ◽  
Mycorrhizal Fungi ◽  
Parental Species ◽  
Reaction Products ◽  
Chain Reaction ◽  
Short Term ◽  
Orchid Species ◽  
Ecological Preferences ◽  
Orchid Mycorrhizal Fungi ◽  
Polymerase Chain

Abstract The nuclear ribosomal DNAwas used to identify the orchid mycorrhizal fungi found in roots of Orchis xbivonae and its parental species Orchis anthropophora and Orchis italica. Polymerase chain reaction products were sequenced and identified using the expanded database.We determined that closely related Tulasnellaceae are mycorrhizal in the three orchid taxa, suggesting that the mycorrhizal partner does not impair hybrid survival. This study demonstrates that O. xbivonae displays few differences in comparison with its two parental species in identity of its associated mycorrhizal fungi, it is a short- -term by-product of the hybridizing behavior of common pollinators, and thus it will not easily origin descendents with potential new genetic combinations and/or ecological preferences.

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