Trichoderma spp. from Roots of Fumigated Douglas-Fir Trees Infected with Phellinus weirii

Mycologia ◽  
1995 ◽  
Vol 87 (5) ◽  
pp. 639 ◽  
Author(s):  
E. E. Nelson ◽  
W. G. Thies ◽  
M. G. McWilliams
Mycologia ◽  
1995 ◽  
Vol 87 (5) ◽  
pp. 639-642 ◽  
Author(s):  
E. E. Nelson ◽  
W. G. Thies ◽  
M. G. McWilliams

Author(s):  
KAYE RICHARDSON ◽  
GEORGE D WARD ◽  
NEELAM RAVEL ◽  
M L KUMLER ◽  
L M PIKE ◽  
...  

1989 ◽  
Vol 35 (2) ◽  
pp. 283-288 ◽  
Author(s):  
Magdalena Y. Giron ◽  
Jeffrey J. Morrell

The microfungi present in transmission poles of preservative-treated Douglas-fir remedially treated with one of four fumigants were determined by removing increment cores 5 and 15 years later and culturing them on nutrient media. The microfungi isolated from the wood were then characterized according to their ability to cause a loss in wood weight, to tolerate conventional wood preservatives, and to tolerate wood fumigants. The wood treated with fumigants 5 years earlier was sparsely colonized, while that treated 15 years earlier was colonized more heavily. In general, many of the same species of microfungi occurred in treated and untreated poles. In both, fungal populations were dominated by Scytalidium and Trichoderma spp. None of the isolates caused losses in wood weight greater than 5%, but several exhibited tolerance to short fumigant exposures. The latter trait may help explain the presence of these fungi in wood still containing measurable levels of fumigant. The presence in fumigant-treated wood of fungi previously shown to be antagonistic toward wood decay fungi may help explain the ability of the four test fumigants to provide long-term protection.Key words: fungi, Scytalidium, Trichoderma, colonization, Douglas-fir.


2004 ◽  
Vol 94 (11) ◽  
pp. 1235-1243 ◽  
Author(s):  
Arezoo Zamani ◽  
Rona N. Sturrock ◽  
Abul K. M. Ekramoddoullah ◽  
Jun Jun Liu ◽  
Xueshu Yu

In western North America, Douglas-fir (Pseudotsuga menziesii) is the most economically important conifer species susceptible to laminated root rot caused by Phellinus weirii. While attempting to internally sequence an endochitinase found to be up-regulated in P. weirii-infected Douglas-fir roots, we obtained overlapping peptide fragments showing 28% similarity with a PR-5 thaumatin-like protein (TLP) designated PmTLP (Pm for Pseudotsuga menziesi). A rabbit polyclonal antibody was reared against a synthetic peptide composed of a 29-amino-acid-long, conserved, internal sequence of PmTLP and purified by immunoaffinity. Western immunoblot analysis of infected roots of 24-year-old coastalfir showed significantly higher amounts of PmTLP (P < 0.01) closest to the point of P. weirii inoculation and infection than in uninfected regions of the same root. The antibody was also used to screen for PmTLP in roots of 25-year-old interior Douglas-firs naturally infected with a related pathogen, Armillaria ostoyae, and results showed significantly higher levels of PmTLP in bark tissues adjacent to infection (P < 0.05) than in uninfected tissue. Using polymerase chain reaction (PCR)-based cloning, the cDNA of PmTLP was shown to have a 702-bp open reading frame with a signal peptide cleavage site at 155 bp corresponding to a 29-amino-acid-long residue prior to the start of the N-terminal. Based on the deduced amino acid sequence, the molecular mass of the putative PmTLP was calculated to be 21.0 kDa with an isoelectric point of 3.71. Alignment analysis of PmTLP cDNA with a representative genomic DNA PCR sequence showed presence of one intron of variable size, within the coding region. The induction of PmTLP at the site of root infection and its presence in needle tissue suggests a general role for this protein in adaptation to stress and may be part of an integrated defense response initiated by the host to impede further pathogen spread.


1994 ◽  
Vol 9 (2) ◽  
pp. 52-56
Author(s):  
E. E. Nelson ◽  
M. G. McWilliams ◽  
W. G. Thies

Abstract Twelve plots were established in 1972 in an 11-yr-old Douglas-fir (Pseudotsuga menziesii) plantation infected with Phellinus weirii, the cause of laminated root rot. All plots were thinned and either interplanted with red alder (Alnus rubra) or fertilized at 5-10 yr intervals with urea to determine the effect of nitrogen on tree growth and mortality caused by P. weirii, or left untreated. Interplanted alder, however, failed to survive. Mortality was assessed at intervals of 2 to 3 yr. Plots were inventoried (100% cruise) in 1978 and 1990. Growth over 12 yr appeared better on fertilized than nonfertilized plots, but the difference was not significant (alpha = 0.05 ). Mortality caused by the preferential feeding of bears on the inner bark of fertilized trees reduced the overall gain. Mortality caused by laminated root rot did not differ significantly (alpha = 0.05) among treatments. Three months after the initial application of urea at 448 kg N/ha, soil sampled to a depth of 30 cm was higher in ammonium and nitrate forms of nitrogen on fertilized than nonfertilized plots, but increases were not significant (alpha = 0.05). Numbers of bacteria were directly correlated with soil ammonium content (P = 0.1092). Numbers of aerobic actinomycetes were inversely correlated with soil nitrate content (P = 0.0398).West. J. Appl. For. 9(2): 00-00.


Mycologia ◽  
1989 ◽  
Vol 81 (3) ◽  
pp. 375 ◽  
Author(s):  
Barry Goldfarb ◽  
Earl E. Nelson ◽  
Everett M. Hansen

Mycologia ◽  
1989 ◽  
Vol 81 (3) ◽  
pp. 375-381 ◽  
Author(s):  
Barry Goldfarb ◽  
Earl E. Nelson ◽  
Everett M. Hansen

Mycologia ◽  
1987 ◽  
Vol 79 (3) ◽  
pp. 370-374 ◽  
Author(s):  
Earl E. Nelson ◽  
Barry Goldfarb ◽  
Walter G. Thies

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