Suppression of Mycorrhizal Fungus Spore Germination in Non-Sterile Soil: Relationship to Mycorrhizal Growth Response in Big Bluestem

Mycologia ◽  
1989 ◽  
Vol 81 (3) ◽  
pp. 382 ◽  
Author(s):  
B. A. Daniels Hetrick ◽  
G. W. T. Wilson
Keyword(s):  
Spore Germination ◽  
Growth Response ◽  
Mycorrhizal Fungus ◽  
Big Bluestem ◽  
Sterile Soil ◽  
Fungus Spore ◽  
Mycorrhizal Growth Response

Suppression of Mycorrhizal Growth Response of Big Bluestem by Non-Sterile Soil

Mycologia ◽  
1988 ◽  
Vol 80 (3) ◽  
pp. 338-343 ◽  
Author(s):  
G. W. T. Wilson ◽  
B. A. Daniels Hetrick ◽  
D. Gerschefske Kitt
Keyword(s):  
Growth Response ◽  
Big Bluestem ◽  
Sterile Soil ◽  
Mycorrhizal Growth Response

Suppression of Mycorrhizal Growth Response of Big Bluestem by Non-Sterile Soil

Mycologia ◽  
1988 ◽  
Vol 80 (3) ◽  
pp. 338 ◽  
Author(s):  
G. W. T. Wilson ◽  
B. A. Daniels Hetrick ◽  
D. Gerschfeske Kitt
Keyword(s):  
Growth Response ◽  
Big Bluestem ◽  
Sterile Soil ◽  
Mycorrhizal Growth Response

Suppression of vesicular–arbuscular mycorrhizal fungus spore germination by nonsterile soil

1989 ◽  
Vol 67 (1) ◽  
pp. 18-23 ◽  
Author(s):  
G. W. T. Wilson ◽  
B. A. Daniels Hetrick ◽  
D. Gerschefske Kitt
Keyword(s):  
Spore Germination ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Dry Weight ◽  
Available Phosphorus ◽  
Nonsterile Soil ◽  
Surface Sterilization ◽  
Vesicular Arbuscular ◽  
Fungus Spore

When the effect of nonsterile soil on vesicular–arbuscular mycorrhizal fungus spore germination was examined, significantly fewer Glomus etunicatum (22 vs. 63 and 64%) or Glomus mosseae (23 vs. 80 and 79%) spores germinated in nonsterile soil than in autoclaved or pasteurized soil, respectively. In some cases, addition of nonsterile soil sievings to autoclaved or pasteurized soil also reduced germination, as compared with germination in unamended pasteurized or autoclaved soil. Germination was reduced by as much as 45% in autoclaved or pasteurized soil if spores were surface sterilized. However, surface sterilization of G. etunicatum spores did not affect dry weight of inoculated big bluestem plants, but mycorrhizal root colonization was reduced when spore-associated microbes were removed from spores by surface sterilization. The detrimental effect of soil microbes on spore germination and mycorrhizal growth response may reflect microbial competition for nutrients since germination of G. etunicatum and G. mosseae was reduced when pasteurized soil was amended with 15, 30, or 60 ppm phosphorus and 60 ppm phosphorus, respectively. An optimum range of available phosphorus may exist, above or below which germination is suppressed.

scholarly journals Relationship of soil fertility to suppression of the growth response of mycorrhizal big bluestem in non-sterile soil

1988 ◽  
Vol 109 (4) ◽  
pp. 473-481 ◽  
Author(s):  
D. GERSCHEFSKE KITT ◽  
B. A. DANIELS HETRICK ◽  
G. W. T. WILSON
Keyword(s):  
Soil Fertility ◽  
Growth Response ◽  
Big Bluestem ◽  
Sterile Soil ◽  
Relationship Of

In vitro culture of arbuscular mycorrhizal fungus and Frankia for inoculation of micropropagated Casuarina equisetifolia L.

1999 ◽  
Vol 77 (9) ◽  
pp. 1391-1397
Author(s):  
Genevieve Louise Mark ◽  
John E Hooker ◽  
Alexander Hahn ◽  
Chris T Wheeler
Keyword(s):  
Spore Germination ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Dichlorophenoxyacetic Acid ◽  
Casuarina Equisetifolia ◽  
Half Strength ◽  
2,4 Dichlorophenoxyacetic Acid

Micropropagated, rooted, and calli explants of Casuarina equisetifolia L. were inoculated with Frankia UGL 020605S and the arbuscular mycorrhizal fungus (AMF) Glomus mosseae, in single and dual co-culture, in vitro. Different micropropagation media formulations were evaluated for their capacity to stimulate germination of G. mosseae spores and growth of Frankia. Murashige and Skoog basal nutrient (half strength) medium, supplemented with 6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D), and pyruvate was selected for the in vitro co-culture of C. equisetifolia callus explants, G. mosseae, and Frankia. This medium (M4) supported 70% AMF spore germination with 44 and 34% of the germinating spores producing single and branched hyphal strands, respectively. Hoaglands (quarter strength, modified by Hoaglands and Arnon (1950)) nutrient medium (M5) with no supplements was selected for the in vitro co-culture of rooted C. equisetifolia explants, G. mosseae, and Frankia and supported 57% AMF spore germination with 29 and 40% of the germinating spores producing single and branched hyphal strands, respectively. Both media supported significant growth of Frankia. In both cases agar was substituted with Terragreen(r). AMF appressoria and intercellular hyphae were observed in rooted C. equisetifolia at 28 days; arbuscule formation occurred at 56 days postinoculation. Frankia infection was evident after 28 days. This was observed in both dual and single in vitro co-cultures. No specific immunofluorescent or immunogold reactions to monoclonal antibodies (mABs) anti-Frankia < 8C5 > and anti-G. mosseae < F5G5 > were evident in C. equisetifolia callus explants.Key words: arbuscular mycorrhizal fungi (AMF), Frankia, Casuarina, micropropagation, immunofluorescent labelling.

New method for arbuscular mycorrhizal fungus spore separation using a microfluidic device based on manual temporary flow diversion

Mycorrhiza ◽  
2020 ◽  
Vol 30 (6) ◽  
pp. 789-796
Author(s):  
K. Srisom ◽  
P. Tittabutr ◽  
N. Teaumroong ◽  
Y. Lapwong ◽  
R. Phatthanakun ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Flow Diversion ◽  
New Method ◽  
Fungus Spore

scholarly journals Growth of Bacillus subtilis and Spore Germination in Soil Observed by a Fluorescent-antibody Technique

Microbiology ◽  
2000 ◽  
Vol 81 (1) ◽  
pp. 191-198 ◽  
Author(s):  
A. Siala ◽  
T. R. G. Gray
Keyword(s):  
Bacillus Subtilis ◽  
Forest Soil ◽  
Spore Germination ◽  
Fluorescent Antibody ◽  
Fungal Growth ◽  
Fluorescent Antibody Technique ◽  
Alkaline Soil ◽  
Sterile Soil ◽  
Antibody Technique ◽  
Acid Forest Soil

Vegetative bacteria of Bacillus subtilis placed in contact with an acid forest soil initially declined in number but grew after the development of fungal hyphae. Growth did not occur in sterile soil, nor in alkaline forest soil unless fungal growth was stimulated. Spores of B. subtilis would not germinate in the same acid forest soil unless fungal growth took place, and hardly germinated at all in the alkaline soil. Roots of seedlings of Pinus sylvestris inhibited both vegetative growth and spore germination. These results are consistent with the observed distribution of vegetative bacteria and spores of B. subtilis in forest soils.

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