Association of G 1 /S-Phase and Late S-Phase Checkpoints with Regulation of Cyclin-Dependent Kinases in Chinese Hamster Ovary Cells

1997 ◽  
Vol 148 (3) ◽  
pp. 260 ◽  
Author(s):  
Joseph A. D' Anna ◽  
Joseph G. Valdez ◽  
Robert C. Habbersett ◽  
Harry A. Crissman
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
S Phase ◽  
Ovary Cells ◽  
Cyclin Dependent Kinases

scholarly journals Cell cycle parameters of Chinese hamster ovary cells during exponential, polyamine-limited growth.

1981 ◽  
Vol 1 (7) ◽  
pp. 594-599 ◽  
Author(s):  
J J Harada ◽  
D R Morris
Keyword(s):  
Cell Cycle ◽  
Chinese Hamster Ovary ◽  
Doubling Time ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
S Phase ◽  
Limited Growth ◽  
Ovary Cells ◽  
Index Analysis ◽  
Thymidine Autoradiography

We have previously shown that Chinese hamster ovary cells made polyamine deficient by treatment with alpha-methylornithine, an inhibitor of ornithine decarboxylase, grow exponentially in culture at low densities at one-half the rate observed in untreated (control) cultures. In this study, the cell cycle of polyamine-limited cells was examined by using thymidine autoradiography, mitotic index analysis, and fraction labeled mitoses analysis. We found that the longer doubling time of inhibitor-treated cultures was a consequence of increases in the lengths of the G1 and S phases. The expansion of the S phase was proportional to the increase in doubling time (twofold), whereas the G1 phase was lengthened by slightly more than a factor of 2. The lengths of the G2 and M phases were essentially unchanged. Putrescine stimulated the growth of inhibitor-treated cultures and restored the cell cycle parameters to those of untreated cells.

Patterns of protein synthesis during the cell cycle of Chinese hamster ovary cells

1987 ◽  
Vol 65 (3) ◽  
pp. 219-229 ◽  
Author(s):  
J. Tim Westwood ◽  
Robert B. Church ◽  
Emile B. Wagenaar
Keyword(s):  
Cell Cycle ◽  
Protein Synthesis ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
S Phase ◽  
Specific Cell ◽  
Ovary Cells ◽  
Temporal Properties ◽  
Gradient Gel Electrophoresis

The protein synthesis patterns at various stages of the cell cycle of Chinese hamster ovary cells were examined by labelling cells with [35S]methionine and then separating the proteins by isoelectric focussing and two-dimensional, nonequilibrium pH gradient gel electrophoresis. We have observed a number of proteins which display quantitative differences in synthesis at specific cell cycle stages and of these the α- and β-tubulins have been identified. A few proteins appear to be uniquely synthesized at specific times during the cell cycle. These include the histones and a modified version of them, which are synthesized only in S phase, and a pair of 21 kilodalton (kDa), pI 5.5 proteins, which appear only in late G2 and mitosis. We have also identified a 58-kDa, pI 7.5 protein which is present at all cell cycle stages except during late G2. This protein appears to have the same temporal properties as a 57-kDa protein called "cyclin" originally described in sea urchin embryos.

Cell cycle parameters of Chinese hamster ovary cells during exponential, polyamine-limited growth

1981 ◽  
Vol 1 (7) ◽  
pp. 594-599
Author(s):  
J J Harada ◽  
D R Morris
Keyword(s):  
Cell Cycle ◽  
Chinese Hamster Ovary ◽  
Doubling Time ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
S Phase ◽  
Limited Growth ◽  
Ovary Cells ◽  
Index Analysis ◽  
Thymidine Autoradiography

We have previously shown that Chinese hamster ovary cells made polyamine deficient by treatment with alpha-methylornithine, an inhibitor of ornithine decarboxylase, grow exponentially in culture at low densities at one-half the rate observed in untreated (control) cultures. In this study, the cell cycle of polyamine-limited cells was examined by using thymidine autoradiography, mitotic index analysis, and fraction labeled mitoses analysis. We found that the longer doubling time of inhibitor-treated cultures was a consequence of increases in the lengths of the G1 and S phases. The expansion of the S phase was proportional to the increase in doubling time (twofold), whereas the G1 phase was lengthened by slightly more than a factor of 2. The lengths of the G2 and M phases were essentially unchanged. Putrescine stimulated the growth of inhibitor-treated cultures and restored the cell cycle parameters to those of untreated cells.

Comparison of purine and pyrimidine metabolism in G1 and S phases of HeLa and Chinese hamster ovary cells

1982 ◽  
Vol 60 (4) ◽  
pp. 422-433 ◽  
Author(s):  
Joyce Hordern ◽  
J. Frank Henderson
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
S Phase ◽  
Pyrimidine Metabolism ◽  
Ovary Cells ◽  
Size Dependent ◽  
Quantitative Studies ◽  
Deoxyribonucleoside Triphosphate ◽  
Rna And Dna

Detailed quantitative studies of purine and pyrimidine metabolism in G1 and S phases of synchronized HeLa and Chinese hamster ovary cells have been carried out. Concentrations of ribonucleoside triphosphates increased approximately in proportion to the increase in cell size as cells moved from G1 to S phase. Deoxyribonucleoside triphosphate concentrations were low in G1 phase and increased 2.5- to 10-fold in S phase. Pathways and rates of metabolism of radioactive adenine, guanosine, deoxyadenosine, deoxyguanosine, uridine, cytidine, deoxyuridine, deoxycytidine, and thymidine were determined by measuring the incorporation of each precursor into individual acid-soluble nucleotides and RNA and DNA bases. Cell-cycle or size-dependent differences were detected in many of the parameters studied.

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